米槁根际土壤可培养真菌的促生及拮抗作用研究

doi:10.16088/j.issn.1001-6600.2024041001

广西师范大学学报（自然科学版） ›› 2025, Vol. 43 ›› Issue (1): 20-30.doi: 10.16088/j.issn.1001-6600.2024041001

• “生态保护与资源可持续利用”专辑 • 上一篇下一篇

米槁根际土壤可培养真菌的促生及拮抗作用研究

陈敬忠^1,2, 彭靓³, 廖小锋⁴, 刘济明⁵, 童炳丽^6*

1.贵州中医药大学药学院, 贵州贵阳 550025;
2.贵州省中药生药学重点实验室(贵州中医药大学), 贵州贵阳 550025;
3.蓬安县杨家镇农业服务中心, 四川南充 637800;
4.贵州科学院贵州省山地资源研究所, 贵州贵阳 550025;
5.贵州大学林学院, 贵州贵阳 550025;
6.贵州师范大学生命科学学院, 贵州贵阳 550025

收稿日期:2024-04-10 修回日期:2024-05-24 出版日期:2025-01-05 发布日期:2025-02-07
通讯作者: 童炳丽(1994—),女,贵州播州人,贵州师范大学讲师。E-mail:2976244664@163.com
基金资助:
贵州省基础研究项目(黔科合基础-ZK〔2022〕一般314); 贵州省科技支撑项目(黔科合支撑〔2023〕一般049); 贵州中医药大学博士启动基金(贵中医启动〔2023〕31号); 国家自然科学基金联合基金(U1812403-2)

Scalp and Antagonistic Effects of Cultivatable Growth Promoting Fungi in Rhizosphere Soil of Cinnamomum migao H. W. Li

CHEN Jingzhong^1,2, PENG Liang³, LIAO Xiaofeng⁴, LIU Jiming⁵, TONG Bingli^6*

1. College of Pharmacy, Guizhou University of Traditional Chinese Medicine, Guiyang Guizhou 550025, China;
2. Guizhou Key Laboratory for Raw Material of Traditional Chinese Medicine (Guizhou University of Traditional Chinese Medicine), Guiyang Guizhou 550025, China;
3. Agricultural Service Center in Yangjia Town, Peng’an County, Nanchong Sichuan 637800, China;
4. Institute of Mountain Resources, Guizhou Academy of Sciences, Guiyang Guizhou 550025, China;
5. College of Forestry, Guizhou University, Guiyang Guizhou 550025, China;
6. College of Life Sciences, Guizhou Normal University, Guiyang Guizhou 550025, China

Received:2024-04-10 Revised:2024-05-24 Online:2025-01-05 Published:2025-02-07

摘要/Abstract

摘要： 为提高珍稀药用植物米槁人工栽培成功率,本研究对从米槁根际分离得到109种真菌的溶磷、解钾、产IAA(吲哚-3-乙酸)、产嗜铁素等潜在促生能力进行筛选评价,寻找高效的米槁幼苗促生方法。采用平板培养法对真菌溶磷、解钾、产IAA及产嗜铁素能力进行评估,对筛选得到同时具有4种促生作用的菌株进行两两对峙,并评估其拮抗作用及酶活性差异。结果表明,仅有44种真菌具有溶磷、解钾、产IAA、产嗜铁素中的一种或多种促生能力,其中Neiria polythalama、Thanatephorus cucumeris、Brunneochlamydosporium cucumeris、Scedosporium boydii以及Gongronella butleri 5种真菌同时具有4种促生能力。5种真菌两两对峙结果显示,仅B. cibotii LD19与S. boydii XL1的对峙没有出现明显拮抗带以及色素带,二者对峙时酶活性有明显的提高;B. ciboti LD19与S. boydii XL1之间不存在明显的拮抗作用且具有较好的促生能力,可成为理想的促生菌株复配方案。

关键词: 米槁, 根际土壤, 真菌, 促生作用, 拮抗作用

Abstract: To promote and assess the feasibility of artificial cultivation of endangered medicinal plant Cinnamomum migao H. W. Li, this study filtered and evaluated the potential growth-promoting abilities of 109 fungi isolated from the rhizosphere of C. migao. The fungi were filter for their abilities to solubilize phosphate, release potassium, produce indole-3-acetic acid (IAA), and produce siderophores. Plate culture method was employed to evaluate the fungal strains’ abilities in phosphate solubilization, potassium release, IAA production, and siderophore production. The fungal strains that simultaneously exhibited all four growth-promoting activities were subjected to pairwise confrontation and evaluated for antagonistic effects and enzymatic activity. The results showed that 44 fungi strains exhibited abilities in phosphate solubilization, potassium release, IAA production, and siderophore production. N. polythalama, T. cucumeris, B. cucumeris, S. boydii, and G. butleri possessed all growth-promoting abilities. In the pairwise confrontation experiments, only the confrontation between B. cibotii LD19 and S. boydii XL1 did not have obvious antagonistic zones or pigment zones, with improved enzymatic activity during their confrontation. There was no significant antagonistic effect between B. cibotii LD19 and S. boydii XL1, and they demonstrated good growth-promoting abilities, making them an ideal combination for a growth-promoting strain formula.

Key words: Cinnamomum migao, rhizosphere soil, fungi, growth-promoting effect, antagonistic effect

中图分类号: S154.3;S567.19

陈敬忠, 彭靓, 廖小锋, 刘济明, 童炳丽. 米槁根际土壤可培养真菌的促生及拮抗作用研究[J]. 广西师范大学学报（自然科学版）, 2025, 43(1): 20-30.

CHEN Jingzhong, PENG Liang, LIAO Xiaofeng, LIU Jiming, TONG Bingli. Scalp and Antagonistic Effects of Cultivatable Growth Promoting Fungi in Rhizosphere Soil of Cinnamomum migao H. W. Li[J]. Journal of Guangxi Normal University(Natural Science Edition), 2025, 43(1): 20-30.

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米槁根际土壤可培养真菌的促生及拮抗作用研究

Scalp and Antagonistic Effects of Cultivatable Growth Promoting Fungi in Rhizosphere Soil of Cinnamomum migao H. W. Li

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