广西师范大学学报（自然科学版） ›› 2017, Vol. 35 ›› Issue (1): 104-112.doi: 10.16088/j.issn.1001-6600.2017.01.017

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AMF对金橘苗根围土壤酶活性及植株生长的影响

康福丽1,2,朱国政1,2,林钰1,2,胡振兴1,2,邓荫伟1,2,冯玉能3,陈胜华3,陈付林3,刘灵1,2   

  1. 1.广西师范大学珍稀濒危动植物生态与环境保护教育部重点实验室,广西桂林541006;
    2.广西师范大学生命科学学院,广西桂林541006;
    3.广西师范大学校园管理中心,广西桂林541004
  • 出版日期:2017-01-20 发布日期:2018-07-17
  • 通讯作者: 刘灵(1968—),女,江西吉安人,广西师范大学副教授,博士。E-mail:liuling@mailbox.gxnu.edu.cn
  • 基金资助:
    广西自然科学基金(2013GXNSFAA019094);广西高校科学技术研究重点项目(ZD2014015);桂林市科学研究与技术开发计划项目(20120119-4)

Effects on Rhizospheric Soil Enzyme Activities and Growth of Fortunella margarita Seedling Inoculated by Two Arbuscular Mycorrhizal Fungi in Two Types of Soil

KANG Fuli1,2, ZHU Guozheng1,2, LIN Yu1,2,HU Zhenxing1,2, DENG Yingwei1,2, FENG Yuneng3, CHEN Shenghua3,CHEN Fulin3, LIU Ling1,2   

  1. 1.Key Laboratory of Ecology of Rare and Endangered Species and Environmental Conservation of Education Ministry, Guangxi Normal University,Guilin Guangxi 541006,China;
    2.College of Life Science, Guangxi Normal University, Guilin Guangxi 541006,China;
    3. Campus Management Center, Guangxi Normal University, Guilin Guangxi 541004,China
  • Online:2017-01-20 Published:2018-07-17

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摘要： 为合理利用丛枝菌根真菌(arbuscular mycorrhizal fungi,AMF)资源,揭示AM真菌对金橘根围土壤酶活性和植株生长影响的机制,以喀斯特非岩溶区酸性土(AS)和岩溶区石灰土(LS)为基质进行盆栽实验,研究分别接种丛枝菌根真菌AMF的摩西球囊霉(Glomus mosseae, G.m)和幼套球囊霉(Glomus etunicatum,G.e)对金橘幼苗根围土壤酶活性的影响。结果表明:相同类型土壤中分别接种G.m和G.e后,无论何种土壤,G.e处理金橘根围菌根侵染率各指标F、m、v均显著高于G.m处理(P≤0.05),其中酸性土中接种G.e处理的最高;此外,分别接种G.e和G.m后,金橘根围土壤蛋白酶、脲酶、磷酸酶和蔗糖酶活性与土壤有机碳、有机质含量及植株生物量均比其相应的不接种对照显著增高。蛋白酶和脲酶活性变化趋势为:(LS+G.e)>(LS+G.m)>(AS+G.e)>(AS+G.m),蔗糖酶和磷酸酶活性变化则为:(AS+G.e)>(AS+G.m)>(LS+G.e)>(LS+G.m),差异均达显著水平 (P≤0.05)。G.e处理金橘根系土壤蛋白酶活性增幅最大,脲酶第二,磷酸酶最小。土壤有机质和有机碳含量变化均有(AS+G.e)>(LS+G.e)>(LS+G.m)>(AS+G.m)的变化趋势,G.e处理均显著高于G.m处理(P≤0.05)。不论酸性土或石灰土,金橘干物质积累表现为接种G.e处理显著高于G.m处理,表现出与AM菌根侵染率相似的变化趋势。可见,AMF可通过增强金橘根围土壤酶活性,影响土壤中有效养分的转化和土壤肥力,最终促进金橘生长。G.e为筛选出的能与喀斯特地区石灰土和酸性土中生长的金橘共生良好的优势菌种,具有较好的运用潜力以克服喀斯特地区土壤肥力低而导致的作物收成欠佳的难题。

关键词: 喀斯特地区;金橘;丛枝菌根真菌;土壤酶活性;植株生长

Abstract: Arbuscular mycorrhizal fungi(AMF) are ubiquitous fungi distributed widely in soil ecosystems. It has been showed that AMF play an important role in improving soil nutrition and enhancing stress resistance. In order to reasonably use AMF resources, two types of soil such as lime soil (LS) and acid soil (AS) sampling from Karst area were used as potted matrix, and two AMF including Glomus mosseae (G.m) and Glomus etunicatum (G.e) were employed to be investigated respectively. The influence mechanism of AMF on plant biomass and soil enzyme activities in rhizosphere of Fortunella margarita Seedlings of Karst area were also discussed. The results show that, comparsion to the no AMF treatment (-AM),the colonization rate of root system of Fortunella margarita seedlings inoculated with either G.e and G.m increased significantly. No matter in either type soil, the colonization frequency of root system (F), colonization rate of root segment (m) and vesicle abundance of root segment (v) of Fortunella margarita inoculated with G.e are significant higher than those with G.m. The F, m, v of Fortunella margarita seedlings with G.e in AS had the maximum level,and those with G.m in LS were second. In addition, compared with the (-AM) treatment, rhizospheric soil enzyme activities of Fortunella margarita seedlings including protease, urease,invertase and phosphatase, along with soil organic carbon (SOC) content, soil organic matter (SOM) fraction and plant biomass rose significantly. Among them, the trends of activities of protease and urease were listed: (LS+G.e)>(LS+G.m)>(AS+G.e)>(AS+G.m), those of invertase and phosphatase were: (AS+G.e)>(AS+G.m)>(LS+G.e)>(LS+G.m), with a very significant level of all (P≤0.05). Moreover, the increasement of protease and urease activity received the first and second place, respectively, the third was that of invertase, and that of phosphatase was at a minimum level. As to rhizospheric SOC content and SOM fraction of Fortunella margarita seedlings, the change trends were shown below:(AS+G.e)>(LS+G.e)>(LS+G.m)>(AS+G.m). Regardless of what type of soil, contents of SOC and SOM of Fortunella margarita inoculated with G.e treatment were significant higher than those with G.m, similar to the changing trend of the colonization rate. The dry matter accumulation (biomass)of Fortunella margarita seedlings also showed a tendency to promote growth corresponding to the AM fungi inoculation. It is concluded that AM fungal symbiont can stimulate the activities of rhizospheric soil enzyme, facilitate the conversion of available nutrients such as N, P, K and fertility in AS and LS, and promoted plant growth in the end. G.e is the screened potential dominant species which possesses a better efficiency symbiosis with Fortunella margarita seedlings no matter growing in AS and LS and have great application potential in overcoming soil infertility and poor crop output problems of Karst area.

Key words: karst area, Fortunella margarita, arbuscular mycorrhizal fungi, soil enzyme activities, growth

中图分类号: 

  • O629.9
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