鸡屎藤内生真菌活性菌株筛选及其抗菌活性物质研究

doi:10.16088/j.issn.1001-6600.2024042502

摘要/Abstract

摘要： 通过菌丝生长速率法测定5株鸡屎藤内生真菌发酵产物不同溶剂萃取物的抑菌活性,筛选出最佳活性菌株及其抑菌活性萃取层,进一步测定其毒力,并利用硅胶柱层析和活性追踪法分离其活性成分,采用核磁共振(NMR)和气相色谱-质谱联用(GC-MS)技术鉴定化合物结构,同时评价活性成分的抑菌效果。研究结果表明,5株内生真菌中菌株PsJ3的乙酸乙酯萃取层抑菌活性最佳,对供试的7种植物病原真菌的有效中浓度(EC₅₀)在0.086 9~1.104 3 g/L;其中,对甘蔗凤梨病菌的抑制效果最为显著,EC₅₀值为0.086 9 g/L;其次是甘蓝黑斑病菌和茶轮斑病菌,EC₅₀值为0.208 2和0.239 7 g/L;对烟草黑胫病菌抑菌效果最差,EC₅₀值为1.104 3 g/L。从不规则毛霉乙酸乙酯层中分离得到化合物镰刀菌酸(fusaric acid),该化合物对以上7种植物病原真菌均具有显著抑菌活性,EC₅₀值为0.018 0~0.390 5 g/L。相似地,其对甘蔗凤梨病菌的抑菌效果最佳,EC₅₀值为0.018 0 g/L;甘蓝黑斑病菌和茶轮斑病菌次之,EC₅₀值为0.030 3和0.038 2 g/L;对烟草黑胫病菌抑菌效果最差,EC₅₀值为0.390 5 g/L。本研究结果表明,鸡屎藤内生真菌含有广谱的抗植物病原真菌活性次生代谢产物,为鸡屎藤内生真菌在农业抗菌活性方面的应用提供了参考。

关键词: 鸡屎藤, 内生真菌, 次生代谢产物, 抑菌活性, 镰刀菌酸

Abstract: The antifungal activities of different solvent extracts from fermentation products of 5 endophytic fungi isolated from Paederia scandens (Lour.) Merr. were determined by a mycelium growth rate method. The optimal active strain and its extraction layers were screened out, and the virulence was further determined. Based on the bioactivity-guiding, the active compound was separated by silica gel column chromatography, and the structure of the compounds was identified by nuclear magnetic resonance (NMR) and gas chromatography-mass spectrometry (GC-MS). The antifungal effect of active compound was also evaluated. The results showed that the ethyl acetate extraction layer of strain PsJ3 had the best antifungal activity against the 7 tested plant pathogenic fungi, with the effective medium concentration (EC₅₀) ranging from 0.086 9 to 1.104 3 g/L. Among them, there was the most significantantifungal effect on C. paradoxa with the EC₅₀ value at 0.086 9 g/L, Followed by Alternaria oleracea and Pestalotiopsis theae with the EC₅₀ values at 0.208 2 and 0.239 7 g/L. It had the lowest antifugal effect on Phytophthora parasitica var with the EC₅₀ value at 1.104 3 g/L. The active compound fusaric acid was obtained from the ethyl acetate layer of the strain PsJ3. It showed significant antifungal activity against all the above seven plant pathogenic fungi, with EC₅₀ values ranging from 0.018 0 to 0.390 5 g/L. Similarly, it had the best antifungal effect against C. paradoxa, with EC₅₀ value of 0.0180 g/L, followed by A. oleracea and P. theae with the EC₅₀ values of 0.030 3 and 0.038 2 g/L. The Antifungal effect on P. parasitica var was the worst, with the EC₅₀ value at 0.390 5 g/L. The results showed that the endophytic fungus of Paederia scandens (Lour.) Merr. contained a broad spectrum antifungal activity of secondary metabolites against plant pathogenic fungi, and provided a reference for the application of endophytic fungi associated with Paederia scandens (Lour.) Merr. in agricultural antifungal activity.

Key words: Paederia scandens (Lour.) Merr., endophytic fungi, secondary metabolites, antifungal activity, fusaric acid

中图分类号: S476

徐紫薇, 邓业成, 骆海玉, 邓志勇, 韦坚芬, 黄玲玉, 甘萍贵. 鸡屎藤内生真菌活性菌株筛选及其抗菌活性物质研究[J]. 广西师范大学学报（自然科学版）, 2025, 43(1): 31-38.

XU Ziwei, DENG Yecheng, LUO Haiyu, DENG Zhiyong, WEI Jianfen, HUANG Lingyu, GAN Pinggui. Study on the Antifungal Activity of Secondary Metabolites from Active Endophytic Fungi Isolated from Paederia scandens (Lour.) Merr.[J]. Journal of Guangxi Normal University(Natural Science Edition), 2025, 43(1): 31-38.

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