广豆根内生真菌GDG-178代谢产物研究

doi:10.16088/j.issn.1001-6600.2020060902

广西师范大学学报（自然科学版） ›› 2021, Vol. 39 ›› Issue (2): 139-143.doi: 10.16088/j.issn.1001-6600.2020060902

广豆根内生真菌GDG-178代谢产物研究

李本超^1,2, 梁艳^1,2, 覃小芽^1,2, 莫土香^1,2, 徐照隆^1,2, 李俊^1,2, 杨瑞云^1,2*

1.广西师范大学化学与药学学院,广西桂林541004;
2.省部共建药用资源化学与药物分子工程国家重点实验室/广西民族药省部共建协同创新中心广西师范大学,广西桂林541004

收稿日期:2020-06-09 修回日期:2020-09-10 出版日期:2021-03-25 发布日期:2021-04-15
通讯作者: 杨瑞云(1978—),女,广西桂林人,广西师范大学研究员,博士。E-mail:yang_rui_yun@163.com
基金资助:
国家自然科学基金(21762007);广西自然科学基金(2018GXNSFAA281169);药用资源化学与药物分子工程国家重点实验室资助项目(CMEMR2018-C29)

Secondary Metabolites of Endophytic Fungus GDG-178 from Sophora tonkinensis

LI Benchao^1,2, LIANG Yan^1,2, QIN Xiaoya^1,2, MO Tuxiang^1,2, XU Zhaolong^1,2, LI Jun^1,2, YANG Ruiyun^1,2*

1. College of Chemistry and Pharmaceutical Sciences, Guangxi Normal University, Guilin Guangxi 541004, China;
2. State Key Laboratory for the Chemistry and Molecular Engineering of Medicinal Resources, Collaborative Innovation Center for Guangxi Ethnic Medicine Guangxi Normal University, Guilin Guangxi 541004, China

Received:2020-06-09 Revised:2020-09-10 Online:2021-03-25 Published:2021-04-15

摘要/Abstract

摘要： 采用硅胶柱层析、 ODS柱层析、 Sephadex LH-20以及半制备HPLC等方法,从广豆根内生真菌GDG-178(Fusarium equiseti)代谢产物中分离得到10个化合物,通过波谱分析,鉴定化合物的结构分别为:白僵菌素 (1)、fusaproliferin (2)、11-epiterpestacin (3)、吲哚-3-乙酸 (4)、indole-3-methylethanoate (5)、4-methyl-5,6-dihydro-2H-pyran-2-one (6)、柄曲霉素 (7)、2′-hydroxydecanylpentadec-5,8,10,12-tetraneoate (8)、19-norergosta-5,7,9,22-tetraene-3β-ol (9)、对羟基苯甲醛 (10)。其中,化合物7~10为首次从镰刀菌属Fusarium sp.中分离得到,化合物5和6为首次从木贼镰刀菌Fusarium equiseti中分离得到。

关键词: 广豆根, 内生真菌, 镰刀菌属, 木贼镰刀菌, 代谢产物

Abstract: Ten compounds were isolated from the metabolites of endophytic fungus GDG-178(Fusarium equiseti)by using silica gel column chromatography, ODS chromatography, SephadexLH-20 and semi-prepared HPLC. The structures of these compounds, beauvericin (1), fusaproliferin (2), 11-epiterpestacin (3), 3-indoleacetic acid (4), indole-3-methylethanoate (5), 4-methyl-5,6-dihydro-2H-pyran-2-one (6), sterigmatocystin (7), 2′-hydroxydecanylpentadec-5,8,10,12-tetraneoate (8), 19-norergosta-5,7,9,22-tetraene-3β-ol (9) and p-hydroxy benzaldehyde (10), were identified by spectral analysis. Compounds 7-10 were obtained from Fusarium sp. for the first time. And compounds 5 and 6 were firstly obtained from Fusarium equiseti.

Key words: Sophora tonkinensis, endophytic fungi, Fusarium sp., Fusarium equiseti, metabolites

中图分类号:

O629.9

李本超, 梁艳, 覃小芽, 莫土香, 徐照隆, 李俊, 杨瑞云. 广豆根内生真菌GDG-178代谢产物研究[J]. 广西师范大学学报（自然科学版）, 2021, 39(2): 139-143.

LI Benchao, LIANG Yan, QIN Xiaoya, MO Tuxiang, XU Zhaolong, LI Jun, YANG Ruiyun. Secondary Metabolites of Endophytic Fungus GDG-178 from Sophora tonkinensis[J]. Journal of Guangxi Normal University(Natural Science Edition), 2021, 39(2): 139-143.

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广豆根内生真菌GDG-178代谢产物研究

Secondary Metabolites of Endophytic Fungus GDG-178 from Sophora tonkinensis

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