广西师范大学学报（自然科学版） ›› 2026, Vol. 44 ›› Issue (6): 146-155.doi: 10.16088/j.issn.1001-6600.2025112003

• 药用资源研究 • 上一篇    下一篇

一株虎舌红来源内生真菌Talaromyces sp.次级代谢产物及抗菌活性研究

吴茜1,2,3,4, 唐海媚1,2,3,4, 植卓坚1,2,3,4, 陈幸1,2,3,4, 王文丽1,2,3,4, 曹越智1,2,3,4, 任佳慧1,2,3,4, 徐伟锋1,2,3,4, 杨瑞云1,2,3,4*   

  1. 1.药用资源化学与药物分子工程教育部重点实验室(广西师范大学), 广西 桂林 541004;
    2.广西药用资源化学与药物分子工程重点实验室(广西师范大学), 广西 桂林 541004;
    3.特色药用资源化学广西高校工程研究中心(广西师范大学), 广西 桂林 541004;
    4.广西师范大学 化学与药学学院, 广西 桂林 541004
  • 收稿日期:2025-11-20 修回日期:2026-01-22 出版日期:2026-11-05 发布日期:2026-11-10
  • 通讯作者: 杨瑞云(1978—),女,广西桂林人,广西师范大学研究员,博士。E-mail: yang_rui_yun@163.com
  • 基金资助:
    广西科技计划(桂科AD25069038);广西自然科学基金(2023GXNSFAA026228, 2025GXNSFAA069913)

Secondary metabolites of an endophytic fungus Talaromyces sp. from Ardisia mamillata Hance and their antibacterial and antifungal activities

Wu Qian1,2,3,4, Tang Haimei1,2,3,4, Zhi Zhuojian1,2,3,4, Chen Xing1,2,3,4, Wang Wenli1,2,3,4, Cao Yuezhi1,2,3,4, RenJiahui1,2,3,4, Xu Weifeng1,2,3,4, Yang Ruiyun1,2,3,4*   

  1. 1. Key Laboratory for Chemistry and Molecular Engineering of Medicinal Resources, Ministry of Education of China (Guangxi Normal University), Guilin Guangxi 541004, China;
    2. Guangxi Key Laboratory of Chemistry and Molecular Engineering of Medicinal Resources (Guangxi Normal University), Guilin Guangxi 541004, China;
    3. Guangxi University Engineering Research Center for Chemistry of Characteristic Medicinal Resources (Guangxi Normal University), Guilin Guangxi 541004, China;
    4. School of Chemistry and Pharmaceutical Sciences, Guangxi Normal University, Guilin Guangxi 541004, China
  • Received:2025-11-20 Revised:2026-01-22 Online:2026-11-05 Published:2026-11-10

摘要： 本文研究一株虎舌红来源内生真菌Talaromyces sp. HSHL-F40的次级代谢产物及抗菌活性。采用柱色谱及半制备高效液相色谱等技术对该菌的大米发酵产物进行分离纯化,通过NMR、HR-ESI-MS等波谱技术对化合物进行结构鉴定,从中共分离鉴定出17个化合物,分别为:(2E,5E)-4-oxo-2-propylidene-5,7-octadienoic acid(1)、peniciisocoumarin H (2)、penicimarin C (3)、penicilloxalone B (4)、(R)-3-((3S,4S)-3,4-dihydroxypentyl)-8-hydroxyisochroman-1-one (5)、(R)-3-((R)-4,5-dihydroxypentyl)-8-hydroxyisochroman-1-one (6)、talaroisocoumarin A(7)、peniciisocoumarin F (8)、aspergillumarins B (9)、aspergillumarin A (10)、(3S,4S)-sclerotinin A (11)、3-(hydroxymethyl)-6,8-dimethoxy-2H-1-benzopyran-2-one (12)、talarohydrazone B (13)、sequoiamonascin C (14)、vermistatin (15)、4-hydroxy-2-methoxyacetanilide (16)、(E)-3-(2,5-dioxo-3-(propan-2-ylidene)pyrrolidin-1-yl)acrylic acid (17),其中,化合物1为新化合物,其余化合物均为首次从虎舌红来源的内生真菌中分离得到。抗菌活性测试结果显示,化合物13对金黄色葡萄球菌、枯草芽孢杆菌、巨大芽孢杆菌均表现出较好的抑制作用,最低抑制浓度(minimal inhibitory concentration,MIC)值均为8 mg/L;化合物1对荔枝炭疽病菌具有中等抗菌活性,MIC值为32 mg/L。

关键词: 虎舌红, 内生真菌, 蓝状菌, 次级代谢产物, 抗菌活性

Abstract: This study investigated the secondary metabolites of the endophyticfungus Talaromyces sp. HSHL-F40 isolated from Ardisia mamillata Hance and their antibacterial and antifungal activities. Seventeen compounds were isolated from the rice fermentation of the fungus by using column chromatography and semi-preparative HPLC. Their structures were identified as(2E,5E)-4-oxo-2-propylidene-5,7-octadienoic acid(1), peniciisocoumarin H (2), penicimarin C (3), penicilloxalone B (4), (R)-3-((3S, 4S)-3, 4-dihydroxypentyl)-8-hydroxyisochroman-1-one (5), (R)-3-((R)-4, 5-dihydroxypentyl)-8-hydroxyisochroman-1-one (6), talaroisocoumarin A (7), peniciisocoumarin F (8), aspergillumarins B (9), aspergillumarin A (10), (3S, 4S)-sclerotinin A (11), 3-(hydroxymethyl)-6, 8-dimethoxy-2H-1-benzopyran-2-one (12), talarohydrazone B(13), sequoiamonascin C (14), vermistatin (15), 4-hydroxy-2-methoxyacetanilide (16), and (E)-3-(2, 5-dioxo-3-(propan-2-ylidene)pyrrolidin-1-yl)acrylic acid (17), by using NMR and HR-ESI-MS. Among these, compound 1 is a new compound, and the other compounds were isolated from the endophytic fungi of Ardisia mamillata Hance for the first time. Compound13 exhibited antibacterial activity against Staphylococcus aureus, Bacillus subtilis and Bacillus megaterium, with MIC values of 8 mg/L. Antifungal assays revealed that compound 1 showed moderate inhibitory activity against Colletotrichum fructicola, with an MIC value of 32 mg/L.

Key words: Ardisia mamillata Hance, endophytic fungi, Talaromyces sp., secondary metabolites, antibacterial and antifungal activity

中图分类号:  O629.9

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