2025年04月05日 星期六

广西师范大学学报（自然科学版） ›› 2025, Vol. 43 ›› Issue (2): 247-257.doi: 10.16088/j.issn.1001-6600.2024082203

• 药用资源研究 • 上一篇    下一篇

嗜盐真菌Cladosporium cladosporioides GXIMD 00533次级代谢产物及其生物活性研究

傅春青1,2, 梁春祥1,2, 梁莉芬1,2, 高程海1,2, 刘永宏1,2, 徐新亚1,2*   

  1. 1.广西中医药大学 海洋药物研究院, 广西 南宁 530200;
    2.广西海洋药物重点实验室(广西中医药大学), 广西 南宁 530200
  • 收稿日期:2024-08-22 出版日期:2025-03-05 发布日期:2025-04-02
  • 通讯作者: 徐新亚(1981—), 男, 河南项城人, 广西中医药大学研究员, 博士。E-mail: xuxy@gxtcmu.edu.cn
  • 基金资助:
    国家自然科学基金(42066006); 广西自然科学基金(2024GXNSFAA010403,2021GXNSFDA075010); 广西中医药大学桂派杏林拔尖人才资助项目(2022C011); 广西中医药大学“岐黄工程”团队培育项目(202407); 中药学广西一流学科(桂教科研〔2022〕1号)

Secondary Metabolites and Biological Activities of Halophilic Fungus Cladosporium cladosporioides GXIMD 00533

FU Chunqing1,2, LIANG Chunxiang1,2, LIANG Lifen1,2, GAO Chenghai1,2, LIU Yonghong1,2, XU Xinya1,2*   

  1. 1. Institute of Marine Drugs, Guangxi University of Chinese Medicine, Nanning Guangxi 530200, China;
    2. Guangxi Key Laboratory of Marine Drugs (Guangxi University of Chinese Medicine), Nanning Guangxi 530200, China
  • Received:2024-08-22 Online:2025-03-05 Published:2025-04-02

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摘要： 本文采用高盐度真菌培养基,对广西北海竹林盐场来源枝孢属嗜盐真菌Cladosporium cladosporioides GXIMD 00533的次级代谢产物进行研究。采用硅胶、反相ODS柱色谱和半制备HPLC等分离手段对该菌株发酵产物进行分离纯化,得到16个单体化合物,根据核磁共振波谱数据对分离得到的化合物进行结构鉴定,鉴定为4个内酯类化合物iso-cladospolide B(1)、cladospolide B(2)、pandangolide 1(3)和thiocladospolide F(4),2个甾醇类化合物cladosporisteroid B(5)和(22E,24R)-3β,5α, 9α-三羟基-7,22-麦角甾二烯-6-酮(6),7个二酮哌嗪brevianamide F(7)、N-乙酰-L-苯丙氨酸(8)、环(L-脯氨酸-L-亮氨酸)(9)、环(L-丙氨酸-L-亮氨酸)(10)、环(L-缬氨酸-L-丙氨酸)(11)、环(L-甘氨酸-L-异亮氨酸)(12)和环(L-亮氨酸-L-甘氨酸)(13),1个氨基酸L-色氨酸(14),2个脂肪酸cladosporacid B(15)和(4R,5S,11R,2E)-4,5,11-三羟基-2-十二烯酸甲酯(16)。对所有化合物进行抗氧化、抗菌、α-葡萄糖苷酶抑制活性、细胞毒活性、抗藤壶附着活性和乙酰胆碱酯酶抑制活性评价。结果显示,化合物6和14具有显著的铁离子还原和抗氧化能力,强于阳性对照抗坏血酸(vitamin C, Vc);化合物6对α-葡萄糖苷酶具有一定程度的抑制活性,IC50为2.662 μmol/L;化合物4具有显著的抗藤壶附着活性,抗附着率为100%;部分化合物表现出一定的乙酰胆碱酯酶活性,其中化合物5抑制率达到54.98%。

关键词: 海水盐场, 嗜盐真菌, 枝孢属, 次级代谢产物, 海洋真菌

Abstract: The secondary metabolites of Cladosporium cladosporioides GXIMD 00533 from Guangxi Beihai Zhulin Salt Field, were studied by using high salinity medium. The fungal extract was separated and purified by silica gel, reversed phase ODS column chromatography and semi-preparative high performance liquid chromatography (HPLC) to obtain 16 compounds. Their structures were elucidated by NMR spectral data and identified as four lactone compounds: iso-cladospolide B (1), cladospolide B (2), pandangolide 1 (3), thiocladospolide F (4), two sterol compounds: cladosporisteroid B (5) and (22E,24R)-3β,5α,9α-trihydroxyergosta-7,22-dien-6-one (6), seven diketopiperazine: brevianamide F (7), N-acetyl-L-phenylalanine (8), cyclo (L-Pro-L-Leu) (9), cyclo (L-Ala-L-Leu) (10), cyclo (L-Val-L-Ala) (11), cyclo (L-Gly-L-Ile) (12), cyclo (L-Leu-L-Gly) (13), one amino acid: L-tryptophan (14), and two fatty acids: cladosporacid B (15) and (4R,5S,11R,2E)-4,5,11-trihydroxy-2-dodecenoate (16). The antioxidant, antibacterial, α-Glucosidase inhibitory, cytotoxic activities, anti-barnala adhesion and acetylcholinesterase inhibitory activities of all compounds were evaluated. Compounds 6 and 14 had significant iron ion reduction and antioxidant capacity. Compound 6 exhibited inhibitory activities on α-glucosidase with IC50 of 2.662 μmol·L-1; Compound 4 had significant anti-barnacle adhesion activity, and the anti-adhesion rate was 100%. Some of the compounds showed certain acetylcholinesterase activity, and the inhibitory rate of compound 5 reached 54.98%.

Key words: marine salt field, halophilic fungus, Cladosporium, secondary metabolite, marine fungus

中图分类号:  Q936; TQ28; P745

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