Journal of Guangxi Normal University(Natural Science Edition) ›› 2023, Vol. 41 ›› Issue (3): 221-231.doi: 10.16088/j.issn.1001-6600.2022040803

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Adsorption of Crystalline Violet by Bacillus sp. LM-24 in Deep Sea

XU Xiangwei1,2,3,4,5, ZHANG Jifu6, ZHANG Yun1,2,3,4,7, HU Yunfeng1,2,3,4*   

  1. 1. South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou Guangdong 510301, China;
    2. CAS Key Laboratory of Tropical Marine Biological Resources and Ecology (South China Sea Institute of Oceanology, Chinese Academy of Sciences), Guangzhou Guangdong 510301, China;
    3. Southern Marine Science and Engineering Guangdong Laboratory (Guangzhou), Guangzhou Guangdong 511458, China;
    4. Guangdong Key Laboratory of Marine Materia Medical (South China Sea Institute of Oceanography, Chinese Academy of Sciences), Guangzhou Guangdong 510301, China;
    5. University of Chinese Academy of Sciences, Beijing 100049, China;
    6. Guangdong Provincial Hospital of Chinese Medicine, Guangzhou Guangdong 510120, China;
    7. Equipment Public Service Center, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou Guangdong 510301, China
  • Received:2022-04-08 Revised:2022-05-26 Online:2023-05-25 Published:2023-06-01

Abstract: The crystalline violet was isolated from wastewater by using Bacillus sp. LM-24, a strain selected from deep-sea microorganisms with a dominant adsorption capacity for crystalline violet. The reaction conditions were optimized by single-factor experiments, and the inorganic carrier silica gel and organic carrier resin were selected for its immobilization. The effects of inorganic salts on the adsorption and its adsorption kinetics and adsorption isotherms were analyzed. The results showed that the adsorption of 30 mg/L crystalline violet was 86% removed by the free strain at the optimum adsorbent concentration of 1.216 g/L, optimum pH 9.0 and 30 ℃ for 10 min, the adsorption of immobilized microorganisms was more stable than that of free microorganisms at different pH and concentration. Kinetic measurements showed that all three adsorbents were physically and chemically adsorbed, and isotherm measurements showed that the free and silica immobilized strains fit the Langmuir model better, while the macroporous resin immobilized strains fit the Freundlich model better. The immobilization technique could improve its stability to the external environment, and it could be a potential strain for the decolorization of crystalline violet dye.

Key words: deep sea microorganism, bacillus, adsorption, crystal violet, immobilized microorganism

CLC Number:  X703
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