基于大孔吸附树脂先交联后吸附法固定化脂肪酶

doi:10.16088/j.issn.1001-6600.2020.04.012

Abstract

Abstract: The aim of this research is to immobilize marine lipase adopting macroporous adsorbent resin HPD700 as the carrier and using neopentyl glycol diglycidyl ether as the cross-linking agent. The adsorption single factor experiment and the orthogonal experiment were carried out under the optimal cross-linking conditions of temperature 35 ℃, cross-linking pH5.5, concentration 0.5%, time 2 h. Based upon both single factor experiment and orthogonal experiment, the optimal adsorption conditions were determined as follows: temperature 40 ℃, carrier amount 1.0 g and 2 h. Under this condition, the enzyme activity reached 190.80 U/g, and the enzyme activity recovery rate was 43.04%. Compared with free enzyme, the pH stability of the immobilized enzyme was basically the same, but the optimal reaction temperature of the immobilized enzyme has increased by 15 ℃ and the immobilized enzyme showed much better thermo-stability. The immobilized enzyme also has good operation stability and storage stability, and the immobilized enzyme could maintain 57.38% enzyme activity after continuous use for 5 times; and remain 69.40% enzyme activity after being stored in at 4 ℃ for 60 days. Therefore, immobilization of marine lipase using macroporous adsorbent resin HPD700 and first cross-linking then adsorbed method has a good prospect in the application of industrial enzyme.

Key words: marine lipase, adsorption after cross-linking, macroporous adsorbent resin, immobilization

CLC Number:

Q814.2

LIN Haijiao, ZHANG Jifu, ZHANG Yun, HU Yunfeng. Immobilization of Lipase by Crosslinking and Then AdsorptionMethod Using Macroporous Adsorbent Resin[J].Journal of Guangxi Normal University(Natural Science Edition), 2020, 38(4): 100-108.

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Immobilization of Lipase by Crosslinking and Then AdsorptionMethod Using Macroporous Adsorbent Resin

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