酶法制备福寿螺内脏团抗菌肽

doi:10.16088/j.issn.1001-6600.2020111001

Abstract

Abstract: Pomaea canaliculata, as one of the invasive species in China, has caused serious harm to the agriculture and ecological environment of many provinces in China. In this paper, the antibacterial peptides from the viscera of P. canaliculata were prepared by enzyme method to investigate the resistance of P. canaliculata to environmental microorganisms during invasion. The inhibition of antimicrobial peptides from the viscera of P. canaliculata on Escherichia coli BL21, E.coli DH5α, Vibrio parahaemolyticus and Aeromonas hydrophila were determined by filter paper diffusion method. The results showed that the antibacterial peptides from the visceral of P. canaliculata prepared by trypsin and pepsin had significant inhibitory effects on the four strains. In this study, the result showed that the optimal enzymolysis time of trypsin for E. coli BL21, E. coli DH5α, V. parahaemolyticus and A. hydrophila was all determined to be 4 h. The optimal enzymolysis time of pepsin was determinded to be 4 h for E. coli DH5α and 6 h for E. coli BL21, V. parahaemolyticus and A. hydrophila. This study lays the foundation for further isolation, purification of the crude extract with antibacterial activity from the viscera of P. canaliculata.

Key words: Pomacea canaliculata, antimicrobial peptide, enzymolysis, trypsin, pepsin, invasive species

CLC Number:

TQ936.16

ZHOU Zihao, LIU Yuhan, TAN Yanhong, MENG Yuqing, WU Hongying, HUANG Jinlong, WU Zhengjun. Enzymatic Preparation of Antimicrobial Peptides from the Viscera of Pomacea canaliculata[J].Journal of Guangxi Normal University(Natural Science Edition), 2021, 39(6): 154-161.

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Enzymatic Preparation of Antimicrobial Peptides from the Viscera of Pomacea canaliculata

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