Journal of Guangxi Normal University(Natural Science Edition) ›› 2023, Vol. 41 ›› Issue (1): 143-154.doi: 10.16088/j.issn.1001-6600.2021112401

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Mechanism of Rubus suavissmus S. Lee in Intervention of Type 2 Diabetes Mellitus Based on Network Pharmacology and Molecular Docking Method

LIANG Linpan1,2, LING Xue3, FANG Jiao1, SU Zhiheng3, ZHENG Hua1*   

  1. 1. Life Sciences Institute, Guangxi Medical University, Nanning Guangxi 530021, China;
    2. Liuzhou People’s Hospital, Liuzhou Guangxi 545006, China;
    3. College of Pharmacy, Guangxi Medical University, Nanning Guangxi 530021, China
  • Received:2021-11-24 Revised:2022-01-15 Online:2023-01-25 Published:2023-03-07

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Abstract: To explore the mechanism of Rubus suavissmus S. Lee in the intervention of type 2 diabetes mellitus, type 2 diabetic rats were fed with high-fat and high-sugar diet combined with streptozotocin (STZ) to observe the changes of blood glucose and pancreatic tissue. The active ingredient targets and type 2 diabetes mellitus related genes of Rubus suavissmus S. Lee were searched and screened by systematic pharmacology method, and enrichment analysis was conducted to predict their possible signaling pathways. Molecular docking verification was conducted between the screened core ingredients and the core target, and the molecular mechanism was explored. The results showed that Rubus suavissmus S. Lee significantly reduced blood sugar in diabetic model rats (P<0.05), and at the same time improved its islet cell damage. KEGG pathway enrichment analysis showed that cancer signaling pathway, FOXO signaling pathway and HIF-1 signaling pathway were closely related to type 2 diabetes mellitus. The molecular docking results showed that baicalin, quercetin and caffeic acid may be the material basis for the anti-glycemic treatment of type 2 diabetes by Rubus suavissmus S. Lee, which was related to the regulation of MAPK1, EGFR, SRC, AKT1, PIK3R1 and PTPN11 signaling pathways.

Key words: network pharmacology, molecular docking, Rubus suavissmus S. Lee, type 2 diabetes mellitus, mechanism

CLC Number: 

  • R285
[1] SAEEDI P,PETERSOHN I,SALPEA P,et al. Global and regional diabetes prevalence estimates for 2019 and projections for 2030 and 2045:results from the International Diabetes Federation Diabetes Atlas,9th edition[J]. Diabetes Research and Clinical Practice,2019,157:107843.
[2]YIN B,BI Y M,FAN G J,et al. Molecular mechanism of the effect of Huanglian Jiedu decoction on type 2 diabetes mellitus based on network pharmacology and molecular docking[J]. Journal of Diabetes Research,2020,2020:5273914.
[3]MERCADO A A,COBO-VUILLEUMIER N,MARTIN E S. Emerging therapeutic targets in regenerative medicine for the treatment of diabetes mellitus: a patent literature review[J]. Recent Patents on Regenerative Medicine,2013,3(1):56-62.
[4]李树刚. 甜茶,悬钩子属一新种[J]. 广西植物,1981(4):17-19.
[5]闫志刚,蒙淑洁,韦荣昌,等. 广西甜茶研究与应用现状[J]. 中草药,2017,48(12):2572-2578,2588.
[6]柏芸,薛淑静,周明,等. 蔷薇科甜茶加工应用研究进展[J]. 农产品加工(创新版),2010(11):52-55.
[7]郑华,苏志恒. 广西甜茶的药理学研究进展[J]. 广西医科大学学报,2015,32(1):149-151.
[8]SU Z H, LING X, JI K W,et al. 1H NMR-based urinary metabonomic study of the antidiabetic effects of Rubus suavissimus S. Lee in STZ-induced T1DM rats[J]. Journal of Chromatography B,2020,1158:122347.
[9]梁小庆,石涛,谢培,等. 甜茶素对四氧嘧啶糖尿病大鼠血糖的影响[J]. 中外医疗,2008,27(35):7-8.
[10]HOPKINS A L. Network pharmacology[J]. Nature Biotechnology,2007,25(10):1110-1111.
[11]王伟,刘星雨,尚云龙,等. 基于血清药物化学与网络药理学探究麝香通心滴丸治冠心病的机制[J]. 中成药,2020,42(10):2768-2777.
[12]毕艺鸣,殷贝,夏亚情,等. 基于网络药理学探讨四逆散治疗2型糖尿病的作用机制[J].中国实验方剂学杂志,2020,26(24):169-177.
[13]郑华,计可为,周莲清,等.甜茶素对棕榈酸诱导INS-1细胞超微结构及Cyt C易位表达的保护作用[J].中国民族民间医药,2015,24(24):16-18,26.
[14]ZHENG H,WU J X,HUANG H,et al. Metabolomics analysis of the protective effect of rubusoside on palmitic acid-induced lipotoxicity in INS-1 cells using UPLC-Q/TOF MS[J]. Molecular Omics,2019,15(3):222-232.
[15]ZHANG R X,XING B H,ZHAO J Y,et al. Astragaloside IV relieves gestational diabetes mellitus in genetic mice through reducing hepatic gluconeogenesis[J]. Canadian Journal of Physiology and Pharmacology,2020,98(7):466-472.
[16]ORSLIC'N,SIROVINA D,ODEH D,et al. Efficacy of caffeic acid on diabetes and its complications in the mouse[J]. Molecules,2021,26(11):3262.
[17]SALEM A M,RAGHEB A S,HEGAZY M G A,et al. Caffeic acid modulates miR-636 expression in diabetic nephropathy rats[J]. Indian Journal of Clinical Biochemistry,2019,34(3):296-303.
[18]EBRAHIMPOUR S,ZAKERI M,ESMAEILI A. Crosstalk between obesity,diabetes,and Alzheimer’s disease:introducing quercetin as an effective triple herbal medicine[J]. Ageing Research Reviews,2020,62:101095.
[19]LI D,JIANG C J,MEI G B,et al. Quercetin alleviates ferroptosis of pancreatic β cells in type 2 diabetes[J]. Nutrients,2020,12(10):2954.
[20]YANG H,YANG T T,HENG C,et al. Quercetin improves nonalcoholic fatty liver by ameliorating inflammation,oxidative stress,and lipid metabolism in db/db mice[J]. Phytotherapy Research,2019,33(12):3140-3152.
[21]HU T,SHI J J,FANG J S,et al. Quercetin ameliorates diabetic encephalopathy through SIRT1/ER stress pathway in db/db mice[J]. Aging (Albany NY),2020,12(8):7015-7029.
[22]徐勇,黄颂敏. MAPK家族与糖尿病并发症[J]. 国外医学:内分泌学分册,2001,21(1):8-10.
[23]李芳,曾欧,罗健,等. 硫化氢对糖尿病大鼠心肌纤维化及MAPK1/3和MMP-8表达的影响[J]. 南方医科大学学报,2015,35(4):549-552.
[24]PENG J,LI Q D,LI K Y,et al. Quercetin improves glucose and lipid metabolism of diabetic rats:involvement of Akt signaling and SIRT1[J]. Journal of Diabetes Research,2017,2017:3417306.
[25]刘桠,张翕宇,晁俊,等. 参芪复方对2型糖尿病GK大鼠胰岛β细胞功能的影响[J]. 中国实验方剂学杂志,2020,26(22):34-39.
[26]LI Y,PAN Y,CAO S R,et al. Podocyte EGFR inhibits autophagy through upregulation of rubicon in type 2 diabetic nephropathy[J]. Diabetes,2021,70(2):562-576.
[27]MYERS M G,MENDEZ R,SHI P,et al. The COOH-terminal tyrosine phosphorylation sites on IRS-1 bind SHP-2 and negatively regulate insulin signaling[J]. Journal of Biological Chemistry,1998,273(41):26908-26914.
[28]YOON S Y,KANG H J,AHN D,et al. Identification of chebulinic acid as a dual targeting inhibitor of protein tyrosine phosphatases relevant to insulin resistance[J]. Bioorganic Chemistry,2019,90:103087.
[29]SEPEHRI Z,KIANI Z,NASIRI A A,et al. Toll-like receptor 2 and type 2 diabetes[J]. Cellular and Molecular Biology Letters,2016,21:2.
[30]LAMPROPOULOU I T,STANGOU Μ,SARAFIDIS P,et al. TNF-α pathway and T-cell immunity are activated early during the development of diabetic nephropathy in type II diabetes mellitus[J]. Clinical Immunology,2020,215:108423.
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