基于网络药理学和分子对接探讨瑶山甜茶治疗2型糖尿病的作用机制

doi:10.16088/j.issn.1001-6600.2021112401

Abstract

Abstract: To explore the mechanism of Rubus suavissmus S. Lee in the intervention of type 2 diabetes mellitus, type 2 diabetic rats were fed with high-fat and high-sugar diet combined with streptozotocin (STZ) to observe the changes of blood glucose and pancreatic tissue. The active ingredient targets and type 2 diabetes mellitus related genes of Rubus suavissmus S. Lee were searched and screened by systematic pharmacology method, and enrichment analysis was conducted to predict their possible signaling pathways. Molecular docking verification was conducted between the screened core ingredients and the core target, and the molecular mechanism was explored. The results showed that Rubus suavissmus S. Lee significantly reduced blood sugar in diabetic model rats (P<0.05), and at the same time improved its islet cell damage. KEGG pathway enrichment analysis showed that cancer signaling pathway, FOXO signaling pathway and HIF-1 signaling pathway were closely related to type 2 diabetes mellitus. The molecular docking results showed that baicalin, quercetin and caffeic acid may be the material basis for the anti-glycemic treatment of type 2 diabetes by Rubus suavissmus S. Lee, which was related to the regulation of MAPK1, EGFR, SRC, AKT1, PIK3R1 and PTPN11 signaling pathways.

Key words: network pharmacology, molecular docking, Rubus suavissmus S. Lee, type 2 diabetes mellitus, mechanism

CLC Number:

R285

LIANG Linpan, LING Xue, FANG Jiao, SU Zhiheng, ZHENG Hua. Mechanism of Rubus suavissmus S. Lee in Intervention of Type 2 Diabetes Mellitus Based on Network Pharmacology and Molecular Docking Method[J].Journal of Guangxi Normal University(Natural Science Edition), 2023, 41(1): 143-154.

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Mechanism of Rubus suavissmus S. Lee in Intervention of Type 2 Diabetes Mellitus Based on Network Pharmacology and Molecular Docking Method

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