Journal of Guangxi Normal University(Natural Science Edition) ›› 2021, Vol. 39 ›› Issue (5): 198-209.doi: 10.16088/j.issn.1001-6600.2020082201

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Analysis of Network Pharmacology and Confirmation of Mahonia fortunei (Lindl. ) Fedde and Glycyrrhiza uralensis Fisch Decoction for Hepatitis

JIANG Xianghui1*, TAN Rong1, YANG Yongping2, XIAO Qingzong1   

  1. 1. College of Health, Kaili University, Kaili Guizhou 556011, China;
    2. Guizhou Oute Pharmaceutical Co. Ltd., Kaili Guizhou 556011, China
  • Received:2020-08-22 Revised:2020-11-02 Online:2021-09-25 Published:2021-10-19

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Abstract: To explore the potential mechanism of Mahonia fortunei (Lindl.)Fedde and Glycyrrhiza uralensis Fisch decoction for hepatitis based on network pharmacology. TCMSP and TCMID databases are used to collect active ingredients and targets of Mahonia fortunei (Lindl.) Fedde and Glycyrrhiza uralensis Fisch decoction, GeneCards and OMIM databases are used to screen for genes related to hepatitis and to intersect with the target genes of active ingredients in Perilla frutescens and Bidens pilosa L. decoction, a protein mutual network (PPI network) was built by the string website, and the biological process of the intersection target was analyzed by the Reactome database and the KEGG metabolic pathway analysis on the Omicshare platform. A component-target-channel network was built by Cytoscape software, screened core targets and core pathways by conducting network topology analysis method, the core target protein crystal structure was further imported into Autodock software to confirm the molecular docking of the active ingredient and the core target protein. By measuring the biochemical indicators in the acute liver injury model, the mechanism of Mahonia fortunei (Lindl.) Fedde and Glycyrrhiza uralensis Fisch decoction for hepatitis were studied. The result showed that Mahonia fortunei (Lindl.) Fedde and Glycyrrhiza uralensis Fisch decoction contains 95 ingredients, including 872 potential targets. There are 198 targets related to hepatitis in Genecards and OMIM databases. 25 intersection targets were obtained by intersection analysis, and 12 core targets were further obtained from the drug-compound-target network graph. By molecular docking confirmation analysis, three potential lead compounds including Kaempferol, naringenin, and isorhamnetin were screened, and the potential target proteins were confirmed. The experimental results showed that the water extract of Mahonia fortunei (Lindl.) Fedde and Glycyrrhiza uralensis Fisch, kaempferol, naringenin and isorhamnetin can significantly reduce the content of ALT, AST, PNP and MDA in the serum of mice, and increase the content of GSH and SOD. The active ingredients in Mahonia fortunei (Lindl.) Fedde and Glycyrrhiza uralensis Fisch decoction may avoid liver injury by regulation of oxidative stress.

Key words: network pharmacology, Mahonia fortunei (Lindl.)Fedde, Glycyrrhiza uralensis Fisch, hepatitis, molecular confirmation

CLC Number: 

  • R285
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