广西师范大学学报（自然科学版） ›› 2023, Vol. 41 ›› Issue (3): 221-231.doi: 10.16088/j.issn.1001-6600.2022040803

• 研究论文 • 上一篇    下一篇

深海芽孢杆菌Bacillus sp. LM-24对结晶紫的吸附研究

徐湘薇1,2,3,4,5, 张继福6, 张云1,2,3,4,7, 胡云峰1,2,3,4*   

  1. 1.中国科学院 南海海洋研究所, 广东 广州 510301;
    2.中国科学院 热带海洋生物资源与生态重点实验室(中国科学院 南海海洋研究所), 广东 广州 510301;
    3.南方海洋科学与工程广东省实验室(广州), 广东 广州 511458;
    4.广东省海洋药物重点实验室(中国科学院 南海海洋研究所), 广东 广州 510301;
    5.中国科学院大学, 北京 100049;
    6.广东省中医院, 广东 广州 510120;
    7.中国科学院 南海海洋研究所 仪器设备公共服务中心, 广东 广州 510301
  • 收稿日期:2022-04-08 修回日期:2022-05-26 出版日期:2023-05-25 发布日期:2023-06-01
  • 通讯作者: 胡云峰(1890—), 男, 山东日照人, 中国科学院南海海洋研究所研究员, 博士。E-mail: yunfeng.hu@scsio.ac.cn
  • 基金资助:
    国家自然科学基金(21302199); 南方海洋科学与工程广东省实验室(广州)人才团队引进重大专项(GML2019ZD0406)

Adsorption of Crystalline Violet by Bacillus sp. LM-24 in Deep Sea

XU Xiangwei1,2,3,4,5, ZHANG Jifu6, ZHANG Yun1,2,3,4,7, HU Yunfeng1,2,3,4*   

  1. 1. South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou Guangdong 510301, China;
    2. CAS Key Laboratory of Tropical Marine Biological Resources and Ecology (South China Sea Institute of Oceanology, Chinese Academy of Sciences), Guangzhou Guangdong 510301, China;
    3. Southern Marine Science and Engineering Guangdong Laboratory (Guangzhou), Guangzhou Guangdong 511458, China;
    4. Guangdong Key Laboratory of Marine Materia Medical (South China Sea Institute of Oceanography, Chinese Academy of Sciences), Guangzhou Guangdong 510301, China;
    5. University of Chinese Academy of Sciences, Beijing 100049, China;
    6. Guangdong Provincial Hospital of Chinese Medicine, Guangzhou Guangdong 510120, China;
    7. Equipment Public Service Center, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou Guangdong 510301, China
  • Received:2022-04-08 Revised:2022-05-26 Online:2023-05-25 Published:2023-06-01

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摘要： 利用深海微生物中筛选出的对结晶紫具有优势吸附能力的菌株Bacillus sp. LM-24从废水中分离结晶紫。采用单因素实验优化其反应条件,选用无机载体硅胶和有机载体树脂对其进行固定,分析无机盐对吸附作用的影响及其吸附动力学与吸附等温线。结果表明游离菌株在最适吸附剂浓度1.216 g/L、pH=9.0、温度30 ℃时,反应10 min对30 mg/L结晶紫去除率可达86%;在不同pH和浓度时,固定化微生物吸附作用较游离微生物更加稳定。动力学测定显示3种吸附剂均存在物理和化学吸附,等温线测定显示游离菌株和硅胶固定化菌株与Langmuir模型拟合度更高,而大孔树脂固定化菌株与Freundlich拟合度更高。Bacillus sp. LM-24对结晶紫具有快速且高效的吸附作用,且固定化技术能有效提高其对外界环境的稳定性,可作为结晶紫染料脱色的潜力开发菌株。

关键词: 深海微生物, 芽孢杆菌, 吸附, 结晶紫, 固定化微生物

Abstract: The crystalline violet was isolated from wastewater by using Bacillus sp. LM-24, a strain selected from deep-sea microorganisms with a dominant adsorption capacity for crystalline violet. The reaction conditions were optimized by single-factor experiments, and the inorganic carrier silica gel and organic carrier resin were selected for its immobilization. The effects of inorganic salts on the adsorption and its adsorption kinetics and adsorption isotherms were analyzed. The results showed that the adsorption of 30 mg/L crystalline violet was 86% removed by the free strain at the optimum adsorbent concentration of 1.216 g/L, optimum pH 9.0 and 30 ℃ for 10 min, the adsorption of immobilized microorganisms was more stable than that of free microorganisms at different pH and concentration. Kinetic measurements showed that all three adsorbents were physically and chemically adsorbed, and isotherm measurements showed that the free and silica immobilized strains fit the Langmuir model better, while the macroporous resin immobilized strains fit the Freundlich model better. The immobilization technique could improve its stability to the external environment, and it could be a potential strain for the decolorization of crystalline violet dye.

Key words: deep sea microorganism, bacillus, adsorption, crystal violet, immobilized microorganism

中图分类号:  X703

[1] WEKOYE J N, WANYONYI W C, WANGILA P T, et al. Kinetic and equilibrium studies of Congo red dye adsorption on cabbage waste powder[J]. Environmental Chemistry and Ecotoxicology, 2020, 2: 24-31. DOI: 10.1016/j.enceco.2020.01.004.
[2] LELLIS B, FÁVARO-POLONIO C Z, PAMPHILE J A, et al. Effects of textile dyes on health and the environment and bioremediation potential of living organisms[J]. Biotechnology Research and Innovation, 2019, 3(2): 275-290. DOI: 10.1016/j.biori.2019.09.001.
[3] BHATIA S C, DEVRAJ S. Pollution control in textile industry[M]. New York: WPI Publishing, 2017: 310-320. DOI: 10.1201/9781315148588.
[4] KHAN S, MALIK A. Toxicity evaluation of textile effluents and role of native soil bacterium in biodegradation of a textile dye[J]. Environmental Science and Pollution Research, 2018, 25(5): 4446-4458. DOI: 10.1007/s11356-017-0783-7.
[5] JIKU M A S, SINGHA A, FARUQUEE M, et al. Toxic wastewater status for irrigation usage at Gazipur and Savar industrial vicinity of Bangladesh[J]. Acta Ecologica Sinica, 2021, 41(4): 358-364. DOI: 10.1016/j.chnaes.2021.07.001.
[6] SOJOBI A O, ZAYED T. Impact of sewer overflow on public health: a comprehensive scientometric analysis and systematic review[J]. Environmental Research, 2022, 203: 111609. DOI: 10.1016/j.envres.2021.111609.
[7] ELGARAHY A M, ELWAKEEL K Z, MOHAMMAD S H, et al. A critical review of biosorption of dyes, heavy metals and metalloids from wastewater as an efficient and green process[J]. Cleaner Engineering and Technology, 2021, 4: 100209. DOI: 10.1016/j.clet.2021.100209.
[8] CARDOSO N F, LIMA E C, ROYER B, et al. Comparison of Spirulina platensis microalgae and commercial activated carbon as adsorbents for the removal of reactive red 120 dye from aqueous effluents[J]. Journal of Hazardous Materials, 2012, 241/242: 146-153. DOI: 10.1016/j.jhazmat.2012.09.026.
[9] RADWAN N R E, HAGAR M, CHAIEB K. Adsorption of crystal violet dye on modified bentonites[J]. Asian Journal of Chemistry, 2020, 28(8): 1643-1647. DOI: 10.14233/ajchem.2016.19725.
[10] GHOREISHIS S M, HAGHIGHI R. Chemical catalytic reaction and biological oxidation for treatment of non-biodegradable textile effluent[J]. Chemical Engineering Journal, 2003, 95(1/3): 163-169. DOI: 10.1016/S1385-8947(03)00100-1.
[11] LU H, WANG X L, ZANG M M, et al. Degradation pathways and kinetics of anthraquinone compounds along with nitrate removal by a newly isolated Rhodococcus pyridinivorans GF3 under aerobic conditions[J]. Bioresource Technology, 2019, 285: 121336. DOI: 10.1016/j.biortech.2019.121336.
[12] GUPTA V K, SUHA S. Application of low-cost adsorbents for dye removal: a review[J]. Journal of Environmental Management, 2009, 90(8): 2313-2342. DOI: 10.1016/j.jenvman.2008.11.017.
[13] CRINI G, LICHTFOUSE E. Advantages and disadvantages of techniques used for wastewater treatment[J]. Environmental Chemistry Letters, 2019, 17(1): 145-155. DOI: 10.1007/s10311-018-0785-9.
[14] 邓华,李秋燕,周瑞爽,等.短毛蓼粉末对Cd(Ⅱ)和Cu(Ⅱ)的吸附研究[J].广西师范大学学报(自然科学版),2021,39(3):102-112.DOI:10.16088/j.issn.1001-6600.2020061103.
[15] KANT R. Adsorption of dye eosin from an aqueous solution on two different samples of activated carbon by static batch method[J]. Journal of Water Resource and Protection, 2012, 4(2): 93-98. DOI: 10.4236/jwarp.2012.42011.
[16] 唐豪,陈荣清,刘虹杉,等.结晶紫脱色菌ZTS-1的分离鉴定及脱色性能研究[J].中南民族大学学报(自然科学版),2020,39(4):355-361.DOI:10.12130/znmdzk.202005.
[17] 荚荣,谢萍,秦易.固定化白腐真菌对多种染料脱色的研究[J].菌物系统,2003,22(2):308-313.DOI:10.3969/j.issn.1672-6472.2003.02.024.
[18] 徐湘薇,张继福,张云,等.一株海洋芽孢杆菌对亚甲基蓝的吸附作用研究[J].青岛科技大学学报(自然科学版),2022(期缺失):页码范围缺失.
[19] LAGERGREN S. Zur theorie der sogenannten adsorption gelster stoffe[J]. Handlingar, 1989, 24: 1-39. DOI: 10.1007/BF01501332.
[20] HO Y S, MCKAY G. Pseudo-second order model for sorption processes[J]. Process Biochemistry, 1999, 34(5): 451-465. DOI: 10.1016/S0032-9592(98)00112-5.
[21] LANGMUIR I. The adsorption of gases on plane surfaces of glass, mica and platinum[J]. Journal of the American Chemical Society, 1918, 40(9): 1361-1403. DOI: 10.1021/ja02242a004.
[22] FREUNDLICH H M. Over the adsorption in solution[J]. The Journal of Physical Chemistry A, 1906, 57: 385-470.
[23] ALENCAR W S, LIMA E C, ROYER B, et al. Application of aqai stalks as biosorbents for the removal of the dye Procion Blue MX-R from aqueous solution[J]. Separation Science and Technology, 2012, 47(3): 513-526. DOI: 10.1080/01496395.2011.616568.
[24] 房宏艳,张润林,吕培颖,等.香蕉皮粉对结晶紫废水的吸附动力学与吸附热力学研究[J].化工新型材料,2014,42(10):146-148,154.
[25] 康彩艳,莫蔚明,蒋治良,等.漓江底泥对活性艳蓝X-BR的吸附行为研究[J].广西师范大学学报(自然科学版),2004,22(4):65-68.DOI:10.3969/j.issn.1001-6600.2004.04.016.
[26] ROYER B, CARDOSO N F, LIMA E C, et al. A useful organofunctionalized layered silicate for textile dye removal[J]. Journal of Hazardous Materials, 2010, 181(1/3): 366-374. DOI: 10.1016/j.jhazmat.2010.05.019.
[27] NANDI B K, GOSWAMI A, PURKAIT M K. Removal of cationic dyes from aqueous solutions by kaolin: kinetic and equilibrium studies[J]. Applied Clay Science, 2009, 42(3/4): 583-590. DOI: 10.1016/j.clay.2008.03.015.
[28] LIU W J, YAO C, WANG M H, et al. Kinetics and thermodynamics characteristics of cationic yellow X-GL adsorption on attapulgite/rice hull-based activated carbon nanocomposites[J]. Environmental Progress & Sustainable Energy, 2013, 32(3): 655-662. DOI: 10.1002/ep.11680.
[29] ROYER B, CARDOSO N F, LIMA E C, et al. A useful organofunctionalized layered silicate for textile dye removal[J]. Hazard Mater, 2010, 181: 366-374. DOI: 10.1016/j.jhazmat.2010.05.019.
[30] 黄徐,黑亮,陈文龙,等.固定化微生物处理氨氮废水的研究进展[J].生态科学,2022,41(1):230-236.DOI:10.14108/j.cnki.1008-8873.2022.01.026.
[31] ARGUN M E, DURSUN S, KARATAS M, et al. Activation of pine cone using Fenton oxidation for Cd(II) and Pb(II) removal[J]. Bioresource Technology, 2008, 99(18): 8691-8698. DOI: 10.1016/j.biortech.2008.04.014.
[32] BULUT Y, AYDIN H. A kinetics and thermodynamics study of methylene blue adsorption on wheat shells[J]. Desalination, 2006, 194(1/3): 259-267. DOI: 10.1016/j.desal.2005.10.032.
[33] YAGUB M T, SEN T K, AFROZE S, et al. Dye and its removal from aqueous solution by adsorption: a review[J]. Advances in Colloid and Interface Science, 2014, 209: 172-184. DOI: 10.1016/j.cis.2014.04.002.
[34] AL-TOHAMY R, ALI S S, LI F H, et al. A critical review on the treatment of dye-containing wastewater: ecotoxicological and health concerns of textile dyes and possible remediation approaches for environmental safety[J]. Ecotoxicology and Environmental Safety, 2022, 231: 113160. DOI: 10.1016/j.ecoenv.2021.113160.
[35] SANTOS S C R, BOAVENTURA R A R. Adsorption of cationic and anionic azo dyes on sepiolite clay: equilibrium and kinetic studies in batch mode[J]. Journal of Environmental Chemical Engineering, 2016, 4(2): 1473-1483. DOI: 10.1016/j.jece.2016.02.009.
[36] 郭辰,韩彪,潘翠,等.假单胞菌去除Cr(Ⅵ)的条件优化[J].广西师范大学学报(自然科学版),2021,39(3):113-121.DOI:10.16088/j.issn.1001-6600.2020090705.
[37] SHINDE A V, PATIL B N. Dye removal from aqueous solution into alternative low cost adsorbent: a review[J]. Journal of Emerging Technologies and Innovative Research, 2021, 8(3): 2313-2321.
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