酶法制备福寿螺内脏团抗菌肽

doi:10.16088/j.issn.1001-6600.2020111001

广西师范大学学报（自然科学版） ›› 2021, Vol. 39 ›› Issue (6): 154-161.doi: 10.16088/j.issn.1001-6600.2020111001

酶法制备福寿螺内脏团抗菌肽

周子豪^1,2,3, 刘禹含^1,2,3, 谭艳红^1,2,3, 蒙玉清^1,2,3, 巫虹颖^1,2,3, 黄锦龙^1,2,3*, 武正军^1,2,3*

1.广西师范大学可持续发展创新研究院, 广西桂林 541006;
2.珍稀濒危动植物生态与环境保护教育部重点实验室(广西师范大学), 广西桂林 541006;
3.广西珍稀濒危动物生态重点实验室(广西师范大学), 广西桂林 541006

收稿日期:2020-11-10 修回日期:2021-02-27 出版日期:2021-11-25 发布日期:2021-12-08
通讯作者: 黄锦龙(1986—), 男, 广西东兴人, 广西师范大学讲师, 博士。E-mail: jl_huang@163.com;武正军(1970—), 男(侗族), 湖南靖州人, 广西师范大学教授, 博士。E-mail: wu_zhengjun@aliyun.com
基金资助:
广西科技基地和人才专项(桂科AD20159042); 广西师范大学重点科学研究计划(2017ZD009); 桂林市科学研究与技术开发计划项目(20190205);国家自然科学基金(31960280);广西自然科学基金(2018GXNSFAA294024)

Enzymatic Preparation of Antimicrobial Peptides from the Viscera of Pomacea canaliculata

ZHOU Zihao^1,2,3, LIU Yuhan^1,2,3, TAN Yanhong^1,2,3, MENG Yuqing^1,2,3, WU Hongying^1,2,3, HUANG Jinlong^1,2,3*, WU Zhengjun^1,2,3*

1. Institute for Sustainable Development and Innovation (Guangxi Normal University) , Guilin Guangxi 541006, China;
2. Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection (Guangxi Normal University), Ministry of Education, Guilin Guangxi 541006, China;
3. Guangxi Key Laboratory of Rare and Endangered Animal Ecology (Guangxi Normal University), Guilin Guangxi 541006, China

Received:2020-11-10 Revised:2021-02-27 Online:2021-11-25 Published:2021-12-08

摘要/Abstract

摘要： 福寿螺Pomacea canaliculata作为外来入侵物种之一,对我国多地的农业及生态环境造成严重危害。为探究福寿螺入侵过程中对环境微生物的抵抗力,本文采用生物酶解法制备福寿螺内脏团中的抗菌肽,并用滤纸片扩散法对大肠杆菌BL21(Escherichia coli BL21)、大肠杆菌DH5α(E.coli DH5α)、副溶血性弧菌Vibrio parahemolyticus、嗜水气单胞菌Aeromonas hydrophila的抑制作用进行测定。结果显示:胰蛋白酶和胃蛋白酶制备的福寿螺内脏团抗菌肽对4株菌均有显著抑制作用。优化酶解时间发现:胰蛋白酶酶解液对大肠杆菌BL21、大肠杆菌DH5α、副溶血性弧菌和嗜水气单胞菌的最佳酶解时间均为4 h;胃蛋白酶酶解液对大肠杆菌DH5α的最佳酶解时间为4 h,对大肠杆菌BL21、副溶血性弧菌和嗜水气单胞菌的最佳酶解时间为6 h。本研究为进一步分离纯化福寿螺内脏团中具有抗菌活性的粗提取物提供实验基础。

关键词: 福寿螺, 抗菌肽, 酶解法, 胰蛋白酶, 胃蛋白酶, 入侵物种

Abstract: Pomaea canaliculata, as one of the invasive species in China, has caused serious harm to the agriculture and ecological environment of many provinces in China. In this paper, the antibacterial peptides from the viscera of P. canaliculata were prepared by enzyme method to investigate the resistance of P. canaliculata to environmental microorganisms during invasion. The inhibition of antimicrobial peptides from the viscera of P. canaliculata on Escherichia coli BL21, E.coli DH5α, Vibrio parahaemolyticus and Aeromonas hydrophila were determined by filter paper diffusion method. The results showed that the antibacterial peptides from the visceral of P. canaliculata prepared by trypsin and pepsin had significant inhibitory effects on the four strains. In this study, the result showed that the optimal enzymolysis time of trypsin for E. coli BL21, E. coli DH5α, V. parahaemolyticus and A. hydrophila was all determined to be 4 h. The optimal enzymolysis time of pepsin was determinded to be 4 h for E. coli DH5α and 6 h for E. coli BL21, V. parahaemolyticus and A. hydrophila. This study lays the foundation for further isolation, purification of the crude extract with antibacterial activity from the viscera of P. canaliculata.

Key words: Pomacea canaliculata, antimicrobial peptide, enzymolysis, trypsin, pepsin, invasive species

中图分类号:

TQ936.16

周子豪, 刘禹含, 谭艳红, 蒙玉清, 巫虹颖, 黄锦龙, 武正军. 酶法制备福寿螺内脏团抗菌肽[J]. 广西师范大学学报（自然科学版）, 2021, 39(6): 154-161.

ZHOU Zihao, LIU Yuhan, TAN Yanhong, MENG Yuqing, WU Hongying, HUANG Jinlong, WU Zhengjun. Enzymatic Preparation of Antimicrobial Peptides from the Viscera of Pomacea canaliculata[J]. Journal of Guangxi Normal University(Natural Science Edition), 2021, 39(6): 154-161.

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酶法制备福寿螺内脏团抗菌肽

Enzymatic Preparation of Antimicrobial Peptides from the Viscera of Pomacea canaliculata

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[1]	梁碧霞,黄锦龙,韩丽霞,武正军. 桂林地区福寿螺冬季繁殖力研究[J]. 广西师范大学学报（自然科学版）, 2019, 37(3): 166-173.
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