2025年04月13日 星期日

广西师范大学学报（自然科学版） ›› 2025, Vol. 43 ›› Issue (1): 75-84.doi: 10.16088/j.issn.1001-6600.2024042903

• “生态保护与资源可持续利用”专辑 • 上一篇    下一篇

桂花精油在延缓衰老中的作用探究

卢小倩1,2, 董秋伶1,2, 李安琪1,2, 吴琼1,2*   

  1. 1.广西高校重点实验室干细胞与医药生物技术(广西师范大学),广西 桂林 541004;
    2.广西师范大学 生命科学学院,广西 桂林 541006
  • 收稿日期:2024-04-29 修回日期:2024-06-23 出版日期:2025-01-05 发布日期:2025-02-07
  • 通讯作者: 吴琼(1984—),女,甘肃平凉人,广西师范大学教授,博士。E-mail:wubinbin02@163.com
  • 基金资助:
    广西重点研发计划项目(挂桂AB21196023)

Study on Role of Osmanthus Essential Oil in Delaying Aging

LU Xiaoqian1,2, DO Qiuling1,2, LI Anqi1,2, WU Qiong1,2*   

  1. 1. Key Laboratory of Stem Cells and Biotechnology in Higher Education of Guangxi (Guangxi Normal University), Guilin Guangxi 541004, China;
    2. College of Life Sciences, Guangxi Normal University, Guilin Guangxi 541006, China
  • Received:2024-04-29 Revised:2024-06-23 Online:2025-01-05 Published:2025-02-07

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摘要： 本研究探讨桂花精油对人神经母细胞瘤SH-SY5Y细胞和急性衰老小鼠模型的抗衰老作用。在体外实验中,本文通过测定桂花精油对SH-SY5Y细胞中衰老标志物的表达及细胞内活性氧(ROS)水平的影响来评估其抗衰老效果,结果表明,2~100 μg/mL的桂花精油对SH-SY5Y细胞无毒性,并能显著降低H2O2诱导的ROS水平及改善衰老细胞特征,包括降低衰老分泌相关表型(SASP)基因和衰老相关基因(P16、P53和P21)的表达,及减少SA-β-半乳糖苷酶(SA-β-gal)的产生。在体内实验中,不同剂量的桂花精油(0.012 5、0.025及0.05 μL/g)能显著提升急性衰老小鼠血清中的抗氧化酶(SOD、CAT)活性,同时降低丙二醛(MDA)和ROS水平。综合体外与体内实验结果,桂花精油通过其强大的抗氧化作用显著延缓了细胞和动物模型的衰老进程,为桂花精油在抗衰老治疗中的应用提供科学依据。

关键词: 桂花, 精油, 抗衰老, 抗氧化活性, 动物模型

Abstract: This study explores the anti-aging effects of osmanthus essential oil on human neuroblastoma SH-SY5Y cells and an acute aging mouse model. In vitro experiments, the anti-aging effects of osmanthus essential oil was assessed by measuring its impact on the expression of aging markers and the levels of reactive oxygen species (ROS) in SH-SY5Y cells. The results indicate that osmanthus essential oil at concentrations of 2-100 μg/mL is non-toxic to SH-SY5Y cells and significantly reduces H2O2-induced ROS levels and improved aging cell characteristics, including reducing the expression of senescence-associated secretory phenotype (SASP) genes and aging-related genes (P16, P53, and P21), and decreasing SA-β-gal production. The vivo experiments showed that different doses of osmanthus essential oil (0.012 5, 0.025, and 0.05 μL/g) significantly increased antioxidant enzyme (SOD, CAT) activities in the serum of acutely aged mice, while reducing MDA and ROS levels. Combining the results in vitro and vivo experiments, osmanthus essential oil significantly delays the aging process in cellular and animal models through its potent antioxidant effects, providing strong scientific evidence for its application in anti-aging treatments.

Key words: Osmanthus fragrans, essential oil, anti-aging, antioxidant activity, animal models

中图分类号:  TQ654.2

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