Journal of Guangxi Normal University(Natural Science Edition) ›› 2023, Vol. 41 ›› Issue (5): 96-104.doi: 10.16088/j.issn.1001-6600.2022092502

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A New C21 Steroidal Glycoside from Gymnema sylvestre and Its Hypoglycemic Activity

LIU Meiyu1, ZHANG Jinyan1, ZHOU Tongxi2, LIAO Guangfeng1, YANG Xinzhou2*, LU Rumei1*   

  1. 1. College of Pharmacy, Guangxi University of Chinese Medicine, Nanning Guangxi 530000, China;
    2. School of Pharmaceutical Sciences, South-Central University for Nationalities, Wuhan Hubei 430074, China
  • Received:2022-09-25 Revised:2022-11-18 Published:2023-10-09

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Abstract: The chemical constituents of Gymnema sylvestre (Retz.) Schult. were isolated and purified by macroporous adsorption resin, silica gel, Sephadex LH-20 and semipreparative high performance liquid chromatography. Their structures were identified by physicochemical properties and spectral data. Sixteen compounds were isolated and identified as sylvepregoside E (1), gymnepregoside H (2), apigenin (3), luteolin (4), phydroxybenzaldehyde (5), phydroxybenzoic acid (6), cinnamic acid (7), caffeic acid (8), 3, 4-dimethoxycinnamic acid (9), transphydroxycinnamic acid ethyl ester (10), 4-O-β-D-glucopyranosylvanillic acid (11), ochuscin C (12), pinoresinol (13), 4-hydroxy-2-nonenoic acid (14), linolenic acid (15), and linoleic acid (16). Among them, compound 1 was a new compound, compounds 2-16 were isolated from this plant for the first time. The results of biological activity showed that compounds 1 and 2 could significantly promote the glucose uptake of L6 cells, which were 1.79 and 1.95 times higher than that of the blank group, respectively.

Key words: gymnema sylvestre, C21 steroidal glycosides, sylvepregoside E, gymnepregoside H, hypoglycemic

CLC Number:  R284; R285
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