Journal of Guangxi Normal University(Natural Science Edition) ›› 2015, Vol. 33 ›› Issue (1): 99-103.doi: 10.16088/j.issn.1001-6600.2015.01.016

Previous Articles     Next Articles

Determination of Three Kinds of Bergenin Derivatives inCissus pterolada Hayata by HPLC

CHEN Ru-yi1,2, LUO Pei1,2, LI Yi-jie1,2, ZHENG Ting3, QIN Jiang-ke1,2   

  1. 1. College of Chemistry and Pharmaceutical Sciences, Guangxi Normal University, Guilin Guangxi 541004, China;
    2. Key Laboratory for the Chemistry and Molecular Engineering of Medicinal Resources of State Education Ministry,Guangxi Normal University, Guilin Guangxi 541004, China;
    3. Guilin Institute of Intelligence Science and Technology,Guilin Guangxi 541002, China
  • Received:2014-12-14 Online:2015-03-15 Published:2018-09-17

PDF (PC)

281

Abstract: To develop a quantitative method for determining bergenin,11-O-galloyl bergenin and 11-O- (4-hydroxyl benzoyl) bergenin in Cissus pteroclada Hayata by HPLC,the determination is performed in gradient separations with a column SinoChrom ODS-BP C18 (5 μm, 4.6 mm × 200 mm) and solvent consisting of MeCN-0.1% formic acid. The flow rate is 0.8 mL/min and the wavelength at 275 nm.The results showed that the bergenin, 11-O-galloyl bergenin and 11-O-(4-hydroxyl benzoyl) bergenin were linear in the range of 0.066 7~0.343 7 μg. And their average recovery were 96.11% (RSD=1.92%), 97.02% (RSD=0.9%), 94.43% (RSD=1.77%), respectively. The method is simple,accurate and could be used to determine the content of this three kinds of bergenin derivatives in Cissus pterolada Hayata.

Key words: Cissus pterolada Hayata, bergenin, 11-O-galloyl bergenin, 11-O-(4-hydroxyl benzoyl) bergenin, HPLC

CLC Number: 

  • R284.1
[1] 中华人民共和国卫生部. 中华人民共和国药典(一部) [S]. 北京: 人民卫生出版社, 1977:158-159.
[2] 卫生部药典委员会. 中国药典(一部) [M]. 北京: 人民卫生出版社, 2010:158.
[3] 广西壮族自治区卫生厅 . 广西中药志[M]. 第二辑. 南宁: 广西人民出版社, 1963: 138.
[4] 陈德昭. 中国植物志 [M]. 北京: 科学出版社, 1994: 80.
[5] NAZIR N, KOUL S, QURISHI M A, et al. Immunomodulatory effect of bergenin and norbergenin against adjuvant-induced arthritis:A flow cytometric study[J]. Journal of Ethnopharmacology, 2007, 112(2):401-405.
[6] ARFAN M, AMIN H, KHAN N, et al. Analgesic and anti-inflammatory activities of 11-O-galloylbergenin[J]. Journal of Ethnopharmacology, 2010, 131(2):502-504.
[7] PRITHIVIRAJ B, SINGH U P, MANICKAM M, et al. Antifungal activity of bergenin, a constituent of Flueggea microcarpa[J]. Plant Pathology, 1997, 46(2):224-228.
[8] SRINIVASAN R, CHANDRASEKAR M J N, NANJAN M J, et al. Antioxidant activity of Caesalpinia digyna root[J]. Journal of Ethnopharmacology, 2007, 113(2): 284-291.
[9] 潘光玉. 四方藤乙酸乙酯部位化学成分的分离与生物活性的初步研究[D],桂林:广西师范大学,2008.
[10] 池翠云,王峰,雷婷,等. 瑶药四方藤化学成分研究[J]. 中药材,2010,33(10):1566-1568.
[1] GUAN Shuiqing, ZHOU Gailian, DONG Jingjing, LIN Qinghua, HUANG Pan, WANG Qian. Simultaneous Determination of Five Nucleosides in Pheretima aspergillum (E. Perrier) by HPLC [J]. Journal of Guangxi Normal University(Natural Science Edition), 2020, 38(3): 85-91.
[2] CHEN Xu, LIANG Chengqin. Spectral Features, α-glucosidase Inhibitory Activities, Antioxidant Activities and Quantitative Determination of Mogroside ⅡA of Siraitia grosvenorii [J]. Journal of Guangxi Normal University(Natural Science Edition), 2019, 37(3): 132-141.
[3] HUANG Honghong , QIU Qin,ZHEN Dandan,ZHEN Hanshen,FAN Xiuchun, YU Lifeng. HPLC Fingerprint of Leaf of Sauropus rostratus Miq. from Different Places [J]. Journal of Guangxi Normal University(Natural Science Edition), 2019, 37(3): 142-147.
[4] JIANG Xianghui, JIANG Tianzhi, WANG Xiang, YUAN Jing. Monosaccharide Constituents of the Polysaccharides from the Leaf of Cyclocarya paliurus [J]. Journal of Guangxi Normal University(Natural Science Edition), 2018, 36(2): 87-93.
[5] QIU Qin,YU Lifeng,CHEN Mingwei,ZHEN Dandan,HUANG Lijing,ZHEN Hanshen,XU Yuhua. Determination of Quercetin, Kaempferol and Rhamnolipid in Stem and Leaf of Sedum emarginatum Migo by HPLC [J]. Journal of Guangxi Normal University(Natural Science Edition), 2018, 36(2): 98-104.
[6] QIU Qin,CHEN Mingwei, ZHEN Hanshen, JIANG Lanlan,FAN Xiuchun. HPLC Fingerprint of Stem of Tetrastigma planicaule(Hook. f.) Gagnep. [J]. Journal of Guangxi Normal University(Natural Science Edition), 2017, 35(2): 73-79.
[7] WU Zhu,TIAN Manman,TAN Qiming, CHEN Zhen, WANG Qiuping, HE Jun. Determination of Ginkgolides in Yinlan Tongluo Oral Liquid by HPLC-RID [J]. Journal of Guangxi Normal University(Natural Science Edition), 2017, 35(2): 87-92.
[8] QIU Qin , ZHEN Hanshen , CHEN Mingwei , HUANG Peiqian , FAN Xiuchun. HPLC Fingerprint of Stem of Sauropus rostratus Miq. [J]. Journal of Guangxi Normal University(Natural Science Edition), 2016, 34(2): 116-121.
[9] ZHAO Ying, LIU Xiaocui, LIAO Zhiming, LU Yongchang. Simultaneously Determination of Three Effective Componentsin Swertia tetraptera Maxim. [J]. Journal of Guangxi Normal University(Natural Science Edition), 2016, 34(1): 118-121.
[10] LAN Yun-hua, JIANG Shao-feng, GONG You-li, LI Yun-fei, XIA Ying-hua, LU Zu-jun. Pollution and Fixed/Transformed Ratio of Paraquat in Water of Huixian Wetland [J]. Journal of Guangxi Normal University(Natural Science Edition), 2014, 32(4): 142-151.
[11] LI Jian-jun, JIA Guo-lun, WANG Jun, LIANG Jian-qiang, YANG Li. Morphology and Quality Components Analysis Comparison of Different Lonicera japonica Germplasms [J]. Journal of Guangxi Normal University(Natural Science Edition), 2013, 31(4): 103-108.
[12] LI Jian-jun, LI Jun-fang, LIANG Jian-qiang, LI Jing-yuan, ZHOU Yan-qing. Measurement and Analysis Benchmark Composition of Lonicera japonica Thunb.Producing in Henan by HPLC [J]. Journal of Guangxi Normal University(Natural Science Edition), 2012, 30(2): 94-98.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
Copyright © Journal of Guangxi Normal University(Natural Science Edition), All Rights Reserved.
Tel: 0773-5857325　E-mail: gxsdzkb@mailbox.gxnu.edu.cn
Powered by Beijing Magtech Co. Ltd