Journal of Guangxi Normal University(Natural Science Edition) ›› 2017, Vol. 35 ›› Issue (2): 87-92.doi: 10.16088/j.issn.1001-6600.2017.02.013

Previous Articles     Next Articles

Determination of Ginkgolides in Yinlan Tongluo Oral Liquid by HPLC-RID

WU Zhu1,2,TIAN Manman1,2,TAN Qiming3, CHEN Zhen2, WANG Qiuping1,2, HE Jun2*   

  1. 1. College of Pharmacy, Guizhou University, Guiyang Guizhou 550025, China;
    2.Research Center of BiochemistryEngineering of Guizhou Province, Guizhou University, Guiyang Guizhou 550025, China;
    3.Tongren PolytechnicCollege, Tongren Guizhou 554300, China
  • Online:2017-07-25 Published:2018-07-25

Abstract: To establish a HPLC-RID method for the determination of bilobide, ginkgolide A, ginkgolide B and ginkgolide C in the Yinlan Tongluo oral liquid.The chromatographic conditions included Hypersil ODS2 column(150 mm×4.6 mm,5 μm), methanol-water(28∶72)at the flow rate 0.8 mL/min of the mobile phase, and at 35 ℃, RID. Respectively, the linear ranges of bilobide, ginkgolide A, ginkgolide B and ginkgolide C were 21.46~107.30, 17.94~89.70, 8.00~40.00 and 11.02~55.10 mg/L. The linear regression equations of bilobide, ginkgolide A, ginkgolide B and ginkgolide C were Y=183.84X-841.32(R2=0.997 1), Y=171.98X-689.03(R2=0.998 1), Y=142.97X-55.797(R2=0.997 1) and Y=178.93X-305.41(R2=0.997 9), respecitively. The average recoveries of bilobide, ginkgolide A, ginkgolide B and ginkgolide C were 103.43%(RSD=1.49%),100.86%(RSD=1.74%),101.78%(RSD=1.39%) and 102.49%(RSD=1.17%), respectively. The HPLC-RID method was precise, reproducible and stable, which can be applied in the quality control of Yinlan Tongluo oral liquid.

Key words: bilobide, ginkgolide A, ginkgolide B, ginkgolide C, Yinlan Tongluo oral liquid, HPLC-RID

CLC Number: 

  • R286
[1] 沈宏伟,肖彦春,车仁国,等. 绞股蓝化学成分研究的现状[J].时珍国医国药,2008,19(7):1561-1564.
[2] 李群峰. 绞股蓝的化学成分与药理作用研究进展[J].光明中医,2009,24(12):2396-2398.
[3] 金亭亭,孙兆林,江蔚新. 绞股蓝化学成分及药理作用研究进展[J].亚太传统医药,2014,10(16):30-32.
[4] 李杰,杨舟,詹琤琤. 绞股蓝化学成分与药理作用研究概况[J].湖北中医杂志,2012,34(10):74-76.
[5] 夏晓晖,张宇,郗砚彬,等. 银杏叶化学成分研究进展[J].中国实验方剂学杂志,2009,15(9):100-104.
[6] 陈西娟,王成章,叶建中.银杏叶化学成分及其应用研究进展[J].生物化学工程,2008,42(4):57-62.
[7] 卢定强,陈钧. 银杏内酯的药理作用[J].江苏理工大学学报(自然科学版),2001,22(2):5-9.
[8] 李砥晖,张秀荣,林天慕. 银杏内酯的药理作用研究[J].第四军医大学吉林军医学院学报,2000,22(2):112-114.
[9] 蒋文潇,王保和. 银杏内酯对缺血性脑血管疾病的药理研究进展[J].辽宁中医杂志,2015,42(2):441-444.
[10] 袁传勋,潘见,胡学桥,等. HPLC-RI法测定银杏叶提取物中4种内酯的含量[J]. 药物分析杂志,2008,28(5):739-741.
[11] 肖飞,谭永霞,王永平,等. 高效液相色谱-蒸发光散射检测法测定银杏叶片中4种萜类内酯含量[J].医药导报,2010,29(5):675-677.
[12] 国家药典委员会. 中华人民共和国药典[S].一部. 北京:中国医药科技出版社,2015:316-317.
No related articles found!
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed   
No Suggested Reading articles found!