Abstract: To establish a HPLC-RID method for the determination of bilobide, ginkgolide A, ginkgolide B and ginkgolide C in the Yinlan Tongluo oral liquid.The chromatographic conditions included Hypersil ODS2 column(150 mm×4.6 mm,5 μm), methanol-water(28∶72)at the flow rate 0.8 mL/min of the mobile phase, and at 35 ℃, RID. Respectively, the linear ranges of bilobide, ginkgolide A, ginkgolide B and ginkgolide C were 21.46~107.30, 17.94~89.70, 8.00~40.00 and 11.02~55.10 mg/L. The linear regression equations of bilobide, ginkgolide A, ginkgolide B and ginkgolide C were Y=183.84X-841.32(R²=0.997 1), Y=171.98X-689.03(R²=0.998 1), Y=142.97X-55.797(R²=0.997 1) and Y=178.93X-305.41(R²=0.997 9), respecitively. The average recoveries of bilobide, ginkgolide A, ginkgolide B and ginkgolide C were 103.43%(RSD=1.49%),100.86%(RSD=1.74%),101.78%(RSD=1.39%) and 102.49%(RSD=1.17%), respectively. The HPLC-RID method was precise, reproducible and stable, which can be applied in the quality control of Yinlan Tongluo oral liquid.

Key words: bilobide, ginkgolide A, ginkgolide B, ginkgolide C, Yinlan Tongluo oral liquid, HPLC-RID