Journal of Guangxi Normal University(Natural Science Edition) ›› 2022, Vol. 40 ›› Issue (6): 237-246.doi: 10.16088/j.issn.1001-6600.2021072302

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Comparison of Microbial Functional Diversity Between Rhizosphere and Non-rhizosphere of Heteroplexis microcephala

WANG Bo1, QIN Fang2, SHI Yancai3*, QIN Huizhen3, DENG Lili3, WEI Jiqing3   

  1. 1. College of Tourism and Landscape Architecture, Guilin University of Technology, Guilin Guangxi 541006;
    2. College of Science, Guangxi Normal University, Guilin Guangxi 541006, China;
    3. Guangxi Institute of Botany, Guangxi Zhuangzu Autonomous Region and the Chinese Academy of Sciences, Guilin Guangxi 541006, China
  • Received:2021-07-23 Revised:2021-09-17 Online:2022-11-25 Published:2023-01-17

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Abstract: To clarify the diversity and difference of microbial metabolic function between rhizosphere and non-rhizosphere, and be helpful for understanding the ecological adaptation of Heteroplexis microcephala and other endemic plants in karst area, five types of representative samples from Yangshuo County, Guangxi Zhuangzu Autonomous Region, were collected to analyze the microbial diversity and metabolic function by Biolog plate methods. The results showed that the values of pH and available K, total N, total P, total K, alkaline hydrolysis nitrogen, exchange calcium, and exchange magnesium content in the rhizosphere were higher than those in the non-rhizosphere. The average color change rate of microorganism in rhizosphere soil was higher than that in non-rhizosphere soil. The diversity index and dominance index of rhizosphere microorganisms in the two distribution sites were basically the same as those in the non-rhizosphere, while those in the other three distribution sites were significantly higher than those in the non-rhizosphere. The carbon sources used in rhizosphere and non-rhizosphere microbes were mainly sugars and carboxylic acids, and ability of utilize carbon source of four rhizosphere microorganisms were higher than those of non-rhizosphere. PCA results showed that sugars were the main carbon source driving the difference between those in rhizosphere and in non-rhizosphere. RAD results showed that differences in soil nutrient concentration did significantly impact the carbon source utilization of microorganisms in rhizosphere. Total potassium, exchange calcium and magnesiumand had a great impact on the carbon source utilization of microorganisms in rhizosphere. In general, the activity of the rhizosphere microorganisms and soil nutrient cycling were improved by secreting compounds such as carboxylic acid and sugar, which become the important way of beneficial micro ecological environment to adapting karst adversity.

Key words: Heteroplexis microcephala, rhizosphere soil, soil microorganism, diversity, Biolog-ECO

CLC Number: 

  • S793.9
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