Journal of Guangxi Normal University(Natural Science Edition) ›› 2021, Vol. 39 ›› Issue (1): 128-135.doi: 10.16088/j.issn.1001-6600.2020021502

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A Study on Genetic Diversity of Camellia huana by Single-copy Nuclear Gene PAL

LI Shuang1,2,3, LIU Shangli1,2,3, PEI Siyu1,2,3, TANG Shaoqing1,2,3*   

  1. 1. Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection (Guangxi Normal University), Ministry of Education, Guilin Guangxi 541006, China;
    2. College of Life Science, Guangxi Normal University, Guilin Guangxi 541006, China;
    3. Guangxi Key Laboratory of Rare and Endangered Animal Ecology (Guangxi Normal University), Guilin Guangxi 541006, China
  • Received:2020-02-15 Revised:2020-03-16 Online:2021-01-25 Published:2021-03-27

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Abstract: Camellia huana is an endangered species which distributed in the evergreen,broad-leaved forests in the limestone hills bordering the northern Guangxi and southern Guizhou province. A single-copy nuclear gene PAL (phenylalanine ammonia-lyase,PAL) was employed to amplify and sequence 120 individuals in 12 populations of C. huana plant,and relevant softwares were used to calculate the corresponding genetic diversity and genetic differentiation coefficients. The results were as follows. The aligned nDNA (PAL) with a consensus length of 660 bp contained seven polymorphic sites. A total of eight haplotypes were identified,and genetic diversity indices of total nucleotide (Pi) and haplotype (Hd) diversity were 0.001 31 and 0.631,respectively. Analysis of molecular variance (AMOVA) further revealed that the majority of genetic variation (91.47%) was examined among populations,while only small amount (8.53%) was partioned within populations. The value of FST was 0.085 31. No significant correlation was detected between genetic and geographic distance which was supported by the result of Mantel test. Low level of genetic diversity and moderate level of genetic differential were detected for C. huana. It is suggested that while strengthening the protection of its natural populations,sampling from multiple cultivated populations for ex-situ conservation to maximize the protection of its genetic diversity.

Key words: Camellia huana, single-copy nuclear gene, genetic diversity, genetic differentiation

CLC Number: 

  • Q94
[1] MING T L,BARTHOLOMEW B.Theaceae[M] // WU Z Y, HONG D Y. Flora of China. Beijing: Science Press,2007: 370.
[2] 贺栋业,李晓宇,王丽丽,等. 金花茶化学成分及药理作用研究进展[J]. 中国实验方剂学杂志,2016,22(3): 231-234.
[3] 安明态. 贵州自然分布山茶属植物的资源现状[J]. 贵州林业科技,2005(2): 26-29.
[4] 谢代祖,牙正高,韩俊严,等. 天峨金花茶分布现状及保护策略研究[J]. 绿色科技,2014(4): 89-91.
[5] 覃海宁,杨永,董仕勇,等. 中国高等植物受威胁物种名录[J]. 生物多样性,2017,25(7): 696-744.
[6] ZHANG X, SU H L, YANG J, et al. Population genetic structure,migration,and polyploidy origin of a medicinal species Gynostemma pentaphyllum (Cucurbitaceae)[J]. Ecology and Evolution,2019,9(19): 11145-11170. DOI:10.1002/ece3.5618.
[7] RIVERS M C,BRUMMITT N A,LUGHADHA E N,et al. Genetic variation in Delonix s.l. (Leguminosae) in Madagascar revealed by AFLPs: fragmentation,conservation status and taxonomy[J]. Conservation Genetics,2011,12(5): 1333-1344. DOI:10.1007/s10592-011-0234-9.
[8] ANDIEGO K,DANGASUK O,ODEE D,et al. Genetic diversity of endangered sandalwood (Osyris lanceolata) populations in Kenya using ISSR molecular markers[J]. East African Agricultural and Forestry Journal,2019,83(2): 80-93. DOI:10.1080/00128325.2019.1605964.
[9] VARDARELI N,DOAROLU T,DOAÇ E,et al. Genetic characterization of tertiary relict endemic Phoenix theophrasti populations in Turkey and phylogenetic relations of the species with other palm species revealed by SSR markers[J]. Plant Systematics and Evolution,2019,305(6): 415-429. DOI:10.1007/s00606-019-01580-8.
[10] 张永德,曾兰,宾石玉,等. 尼罗罗非鱼选育家系的遗传多样性研究[J]. 广西师范大学学报(自然科学版),2014,32(3): 94-101.
[11] GAO Y D, GAO X F, HARRIS A. Species boundaries and parapatric speciation in the complex of alpine shrubs,Rosa sericea (Rosaceae),based on population genetics and ecological tolerances[J]. Frontiers in Plant Science,2019,10: 321. DOI:10.3389/fpls.2019.00321.
[12] HUANG J,ZENG S Y, ZHAO J H, et al. Genetic variation and phylogenetic relationships among Rehmannia (Scrophulariaceae) species as revealed by a novel set of single-copy nuclear gene markers[J]. Biochemical Systematics and Ecology,2016,66: 43-49. DOI:10.1016/j.bse.2016.03.011.
[13] XIAO S Y, JI Y H, LIU J,et al. Genetic characterization of the entire range of Cycas panzhihuaensis (Cycadaceae)[J]. Plant Diversity,2020,42(1): 7-18. DOI:10.1016/j.pld.2019.10.001.
[14] DOYLE J J,DOYLE J L. Isolation of plant DNA from fresh tissue[J]. Focus,1990,12: 13-15.
[15] MATSUMOTO S,TAKEUCHI A,HAYATSU M,et al. Molecular cloning of phenylalanine ammonia-lyase cDNA and classification of varieties and cultivars of tea plants ( Camellia sinensis ) using the tea PAL cDNA probe[J]. Theoretical and Applied Genetics,1994,89(6): 671-675. DOI:10.1007/BF00223703.
[16] WEI S J, LU Y B, YE Q Q, et al. Population genetic structure and phylogeography of Camellia flavida (Theaceae) based on chloroplast and nuclear DNA sequences[J]. Frontiers in Plant Science,2017,8: 718. DOI:10.3389/fpls.2017.00718.
[17] 张文驹,闵天禄. 山茶属古茶组植物的细胞学研究[J]. 云南植物研究,1995, 17(1): 48-54.
[18] CLARK A G. Inference of haplotypes from PCR-amplified samples of diploid populations[J]. Molecular Biology and Evolution,1990,7(2): 111-122.
[19] EDGAR R C. MUSCLE: multiple sequence alignment with high accuracy and high throughput[J]. Nucleic Acids Research,2004,32(5): 1792-1797. DOI:10.1093/nar/gkh340.
[20] TAMURA K,DUDLEY J,NEI M,et al. MEGA4: molecular evolutionary genetics analysis (MEGA) software version 4.0[J]. Molecular Biology and Evolution,2007,24(8): 1596-1599. DOI:10.1093/molbev/msm092.
[21] LIBRADO P,ROZAS J. DnaSP v5: a software for comprehensive analysis of DNA polymorphism data[J]. Bioinformatics,2009,25(11): 1451-1452. DOI:10.1093/bioinformatics/btp187.
[22] TAJIMA F. Statistical method for testing the neutral mutation hypothesis by DNA polymorphism[J]. Genetics,1989,123(3): 585-595.
[23] FU Y X, LI W H. Statistical tests of neutrality of mutations[J]. Genetics,1993,133(3): 693-709.
[24] FU Y X. Statistical tests of neutrality of mutations against population growth,hitchhiking and background selection[J]. Genetics,1997,147(2): 915-925.
[25] PONS O,PETIT R J. Measuring and testing genetic differentiation with ordered versus unordered alleles[J]. Genetics,1996,144(3): 1237-1245.
[26] EXCOFFIER L,LAVAL G,SCHNEIDER S. Arlequin (version 3.0): an integrated software package for population genetics data analysis[J]. Evolutionary Bioinformatics,2005,1: 47-50. DOI:10.1177/117693430500100003.
[27] WRIGHT S. Evolution in mendelian populations[J]. Genetics,1931,16(2): 97-159.
[28] PEAKALL R, SMOUSE P E. GenAlEx 6.5: genetic analysis in Excel. Population genetic software for teaching and research:an update[J]. Bioinformatics,2012,28(19): 2537-2539.
[29] BANDELT H J,FORSTER P,RÖHL A. Median-joining networks for inferring intraspecific phylogenies[J]. Molecular Biology and Evolution,1999,16(1): 37-48. DOI:10.1093/oxfordjournals.molbev.a026036.
[30] CHUNG M Y,LÓPEZ-PUJOL J,SON S,et al. Patterns of genetic diversity in rare and common orchids focusing on the korean peninsula: implications for conservation[J]. The Botanical Review,2018,84(1): 1-25. DOI:10.1007/s12229-017-9190-5.
[31] GUSTAFSSON S,SJÖGREN-GULVE P. Genetic diversity in the rare orchid Gymnadenia odoratissima and a comparison with the more common congener,G. conopsea[J]. Conservation Genetics,2002,3(3): 225-234. DOI:10.1023/A:1019969014333.
[32] LEVY E,BYRNE M,COATES D J,et al. Contrasting influences of geographic range and distribution of populations on patterns of genetic diversity in two sympatric pilbara acacias[J]. PLoS ONE,2016,11(10): e0163995. DOI:10.1371/journal.pone.0163995.
[33] LIU Y,YANG S X, JI P Z, et al. Phylogeography of Camellia taliensis (Theaceae) inferred from chloroplast and nuclear DNA: insights into evolutionary history and conservation[J]. BMC Evolutionary Biology,2012,12(1): 92. DOI:10.1186/1471-2148-12-92.
[34] 陈海玲. 薄叶金花茶及其近缘种遗传多样性和遗传结构研究[D]. 桂林:广西师范大学,2018.
[35] 路雪林. 金花茶的谱系地理学研究[D]. 桂林:广西师范大学,2018.
[36] 陈莉萍. 平果金花茶的保护遗传学研究[D]. 桂林:广西师范大学,2019.
[37] NYBOM H,BARTISH I V. Effects of life history traits and sampling strategies on genetic diversity estimates obtained with RAPD markers in plants[J]. Perspectives in Plant Ecology,Evolution and Systematics,2000,3(2): 93-114. DOI:10.1078/1433-8319-00006.
[38] YANG H S, LI X P, LIU D J, et al. Genetic diversity and population structure of the endangered medicinal plant Phellodendron amurense in China revealed by SSR markers[J]. Biochemical Systematics and Ecology,2016,66: 286-292. DOI:10.1016/j.bse.2016.04.018.
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