Journal of Guangxi Normal University(Natural Science Edition) ›› 2021, Vol. 39 ›› Issue (1): 128-135.doi: 10.16088/j.issn.1001-6600.2020021502

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A Study on Genetic Diversity of Camellia huana by Single-copy Nuclear Gene PAL

LI Shuang1,2,3, LIU Shangli1,2,3, PEI Siyu1,2,3, TANG Shaoqing1,2,3*   

  1. 1. Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection (Guangxi Normal University), Ministry of Education, Guilin Guangxi 541006, China;
    2. College of Life Science, Guangxi Normal University, Guilin Guangxi 541006, China;
    3. Guangxi Key Laboratory of Rare and Endangered Animal Ecology (Guangxi Normal University), Guilin Guangxi 541006, China
  • Received:2020-02-15 Revised:2020-03-16 Online:2021-01-25 Published:2021-03-27

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Abstract: Camellia huana is an endangered species which distributed in the evergreen,broad-leaved forests in the limestone hills bordering the northern Guangxi and southern Guizhou province. A single-copy nuclear gene PAL (phenylalanine ammonia-lyase,PAL) was employed to amplify and sequence 120 individuals in 12 populations of C. huana plant,and relevant softwares were used to calculate the corresponding genetic diversity and genetic differentiation coefficients. The results were as follows. The aligned nDNA (PAL) with a consensus length of 660 bp contained seven polymorphic sites. A total of eight haplotypes were identified,and genetic diversity indices of total nucleotide (Pi) and haplotype (Hd) diversity were 0.001 31 and 0.631,respectively. Analysis of molecular variance (AMOVA) further revealed that the majority of genetic variation (91.47%) was examined among populations,while only small amount (8.53%) was partioned within populations. The value of FST was 0.085 31. No significant correlation was detected between genetic and geographic distance which was supported by the result of Mantel test. Low level of genetic diversity and moderate level of genetic differential were detected for C. huana. It is suggested that while strengthening the protection of its natural populations,sampling from multiple cultivated populations for ex-situ conservation to maximize the protection of its genetic diversity.

Key words: Camellia huana, single-copy nuclear gene, genetic diversity, genetic differentiation

CLC Number: 

  • Q94
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