荧光偏振技术在生化分析检测中的研究进展

doi:10.16088/j.issn.1001-6600.2022030310

Abstract

Abstract: Fluorescence polarization (FP) is an analytical method for the study of intermolecular interaction or quantitative detection of target molecules through the changes of FP signals before and after the combination of fluorescent groups and other molecules in the system. It has significant advantages such as high sensitivity, rapid analysis, easy operation and high throughput, which can be combined with other analytical technologies, and has been widely used in the fields of biochemical analysis and disease diagnosis. In recent years, with the development of modern analytical techniques and technological advances in the fields of nucleic acid and material science, many FP assays with higher analytical performance have been reported. The trend of future researches is to deeply explore the fluorescence polarization detection technology with strong stability, low cost and various functions. This review,mainly summarize the recent advances of FP and their analytical applications in detection of biological small molecules, nucleic acids, proteins, biological enzymes, metal ions, pesticides and biochemically related substances. Moreover, the prospect for future development of FP is provided.

Key words: fluorescent polarization, biochemical analysis, biomolecules, signal amplification, nucleic acid

CLC Number:

O657

ZHANG Yiwen, WEI Wenyan, ZHAO Jingjin. Review on the Applications of Fluorescence Polarization and Its Biochemical Analysis[J].Journal of Guangxi Normal University(Natural Science Edition), 2022, 40(5): 216-226.

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Review on the Applications of Fluorescence Polarization and Its Biochemical Analysis

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