Journal of Guangxi Normal University(Natural Science Edition) ›› 2018, Vol. 36 ›› Issue (1): 99-104.doi: 10.16088/j.issn.1001-6600.2018.01.014

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Determination of Oleanic Acid and Uosolic Acid in Gentiana straminea Maxim. by MEKC

ZHANG Junwen1,2,3,LI Chengsi1,2,3,LU Yongchang1,2,3*,SHI Shenghui1,2,3   

  1. 1.College of Pharmacy,Qinghai Nationalities University,Xining Qinghai 810007,China;
    2.Key Laboratory of Plant Resources Chemical Research of Qinghai-Tibet Plateau in Qinghai Province, Qinghai Nationalities University,Xining Qinghai 810007,China;
    3.Key Laboratory of Pharmaceutical Analysis in Qinghai Province,Qinghai Nationalities University,Xining Qinghai 810007,China
  • Online:2018-01-20 Published:2018-07-17

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Abstract: A micellar electrokinetic chromatography (MEKC) method was established for determining the contents of oleanic acid and ursolic acid in Gentiana straminea Maxim.. The separation was performed on an uncoated fused silica capillary (64.5 cm×50 μm,56 cm of effective length) with a voltage of 15 kV. A 20% acetonitrile solution containing 70 mmol/L borax and 35 mmol/L SDS(pH=9.85) was used as the buffer solution. The sample was injected at a pressure of 5 kPa×5 s and detected at a wavelength of 210 nm.The results showed a good linearity between the concentration and peak area (r>0.999 2). The RSD of two active ingredients were 4.21% and 3.87%. This MEKC method has the advantages of simple,accurate and reproducible. It could be used for the quality evaluation and control for Gentiana straminea Maxim..

Key words: micellar electrokinetic chromatography(MEKC), Gentiana straminea Maxim., oleanic acid, uosolic acid, content

CLC Number: 

  • O657.8
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