HPLC法测定冬虫夏草菌粉及含片中腺苷的含量

Abstract

Abstract: The HPLC method was carried on Kromasil-C₁₈(4.6 mm×250 mm,5 μm),using 0.05 mol·L^-1 potassiun dihydrogen phosphatesolution-methanol (90∶10) as a mobile phase,flow rate 1.0 mL·min^-1,dector 260 nm and the column temperature 35 °C,to determine the content of adenosine in Cordyceps sinensics (Berk) Sacc.and tablets.Adensine showed a good linear relationship in the range of 0.005~0.2 μg(r=0.999 3).The average recovery was 101.19%,RSD=1.45%.The established method is accurate,reliable and specific.The results are stable with good reproducibility and the quality of the tablets can be controlled by the method.

Key words: Cordyceps sinensic(Berk.)Sacc., tablets, adenosine, HPLC

CLC Number:

O652

YU Qing-fen. Determination of Adenosine in Cordyceps sinensis(Berk)Sacc.and Tablets by HPLC[J].Journal of Guangxi Normal University(Natural Science Edition), 2010, 28(2): 65-68.

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Determination of Adenosine in Cordyceps sinensis(Berk)Sacc.and Tablets by HPLC

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