Journal of Guangxi Normal University(Natural Science Edition) ›› 2025, Vol. 43 ›› Issue (6): 152-161.doi: 10.16088/j.issn.1001-6600.2024112105

• Ecology and Environmental Science Research • Previous Articles     Next Articles

Chemical Constituents and Biological Activities of Curcuma kwangsiensis

SU Yuanfeng, LI Liangbo, CHEN Jianhua, ZHANG Jinyan, LU Yuling, MENG Qingai, HE Meiying, LIAO Guangfeng*, LU Rumei*   

  1. College of Pharmacy, Guangxi University of Chinese Medicine, Nanning Guangxi 530200, China
  • Received:2024-11-21 Revised:2025-01-26 Published:2025-11-19

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Abstract: To investigate the chemical constituents and biological activities of Curcuma kwangsiensis, the traditional and modern methods such as silica gel and semi-preparative high performance liquid chromatography were used to isolate and purify the ethanol extract of Curcuma kwangsiensis. Identification of the structure of compounds by modern spectroscopic means, and the anti-tumor and anti-inflammatory activities of the compounds were detected by MTS method and Griess method, respectively. A total of eighteen compounds were isolated from this plant and identified as(3R,5S)-3,5-dihydroxy-1-(4,5-hydroxy-3-methoxy-phenyl)-7-(4-hydroxy-2-methoxy-phenyl) heptane (1), 5-((2S,4R,6S)-4-hydroxy-6-(4-hydroxy-3-methoxyphenethyl)-tetrahydro-2H-pyran-2-yl)-3-methoxybenzene-1,2-diol (2), 5-((2S,4S,6S)-4-hydroxy-6-(4-hydroxy-3-methoxyphenethyl)-tetrahydro-2H-pyran-2-yl)-3-methoxybenzene-1,2-diol (3), broussonin B (4), ellisinin A (5), nyasol (6), genkwanin (7), 2,4-di-tert-butylphenol (8), labda-8(17)-,13(14)-dien-15(16)-olide (9),(E)-labda-8(17),12-dien-15,16-olide (10), glutinol (11), limonin (12), β-sitosterol (13), stigmasterol (14), daucosterol (15), berberine (16), sucrose (17) and palmitic acid (18). Compounds 1-12, 16, 17 were isolated from this plant for the first time, and compounds 4-7, 11, 12, 16 were isolated from Zingiberaceae for the first time. The results of antitumor activity showed that the IC50 of compound 5 against leukemia HL-60, lung cancer A549, liver cancer HepG2, breast cancer MDA-MB-231 and colon cancer SW480 cell lines were 5.11, 44.82, 12.27, 28.82 and 15.78 μmol/L, respectively. The IC50 of compound 6 on leukemia HL-60 and liver cancer HepG2 cells were 20.30 and 35.74 μmol/L, respectively. The IC50 of compound 9 on leukemia HL-60 cells was 34.24 μmol/L, respectively. The IC50 of compound 16 on leukemia HL-60, breast cancer MDA-MB-231 and colon cancer SW480 cells were 6.31, 30.57 and 31.39 μmol/L, respectively. The results of anti-inflammatory activity showed that the IC50 values of compounds 9 and 16 against LPS-induced NO production were 45.76 and 44.67 μmol/L, respectively. The results of this study further enrich the chemical composition library of Curcuma kwangsiensis, and lay a foundation for its further research and development.

Key words: Curcuma kwangsiensis, chemical constituents, anti-tumo, biological activity, anti-inflammatory

CLC Number:  R284
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