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Chemical Constituents and Biological Activities of Curcuma kwangsiensis
SU Yuanfeng, LI Liangbo, CHEN Jianhua, ZHANG Jinyan, LU Yuling, MENG Qingai, HE Meiying, LIAO Guangfeng, LU Rumei
Journal of Guangxi Normal University(Natural Science Edition). 2025, 43 (6):
152-161.
DOI: 10.16088/j.issn.1001-6600.2024112105
To investigate the chemical constituents and biological activities of Curcuma kwangsiensis, the traditional and modern methods such as silica gel and semi-preparative high performance liquid chromatography were used to isolate and purify the ethanol extract of Curcuma kwangsiensis. Identification of the structure of compounds by modern spectroscopic means, and the anti-tumor and anti-inflammatory activities of the compounds were detected by MTS method and Griess method, respectively. A total of eighteen compounds were isolated from this plant and identified as(3R,5S)-3,5-dihydroxy-1-(4,5-hydroxy-3-methoxy-phenyl)-7-(4-hydroxy-2-methoxy-phenyl) heptane (1), 5-((2S,4R,6S)-4-hydroxy-6-(4-hydroxy-3-methoxyphenethyl)-tetrahydro-2H-pyran-2-yl)-3-methoxybenzene-1,2-diol (2), 5-((2S,4S,6S)-4-hydroxy-6-(4-hydroxy-3-methoxyphenethyl)-tetrahydro-2H-pyran-2-yl)-3-methoxybenzene-1,2-diol (3), broussonin B (4), ellisinin A (5), nyasol (6), genkwanin (7), 2,4-di-tert-butylphenol (8), labda-8(17)-,13(14)-dien-15(16)-olide (9),(E)-labda-8(17),12-dien-15,16-olide (10), glutinol (11), limonin (12), β-sitosterol (13), stigmasterol (14), daucosterol (15), berberine (16), sucrose (17) and palmitic acid (18). Compounds 1-12, 16, 17 were isolated from this plant for the first time, and compounds 4-7, 11, 12, 16 were isolated from Zingiberaceae for the first time. The results of antitumor activity showed that the IC50 of compound 5 against leukemia HL-60, lung cancer A549, liver cancer HepG2, breast cancer MDA-MB-231 and colon cancer SW480 cell lines were 5.11, 44.82, 12.27, 28.82 and 15.78 μmol/L, respectively. The IC50 of compound 6 on leukemia HL-60 and liver cancer HepG2 cells were 20.30 and 35.74 μmol/L, respectively. The IC50 of compound 9 on leukemia HL-60 cells was 34.24 μmol/L, respectively. The IC50 of compound 16 on leukemia HL-60, breast cancer MDA-MB-231 and colon cancer SW480 cells were 6.31, 30.57 and 31.39 μmol/L, respectively. The results of anti-inflammatory activity showed that the IC50 values of compounds 9 and 16 against LPS-induced NO production were 45.76 and 44.67 μmol/L, respectively. The results of this study further enrich the chemical composition library of Curcuma kwangsiensis, and lay a foundation for its further research and development.
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