广西师范大学学报（自然科学版） ›› 2026, Vol. 44 ›› Issue (6): 163-174.doi: 10.16088/j.issn.1001-6600.2026011701

• 药用资源研究 • 上一篇    下一篇

基于网络药理学及分子对接探讨三叶青藤醇提物抗佐剂诱导性关节炎大鼠关节损伤的作用机制

赵有为1#, 胡梦凡1,2#, 阳洋1, 梁亮1,3, 肖顺丹1, 冷静1,3,4, 运晨霞1,3*   

  1. 1.广西中医药大学 基础医学院, 广西 南宁 530200;
    2.广西中医药大学 赛恩斯新医药学院, 广西 南宁 530200;
    3.广西特色实验动物模型重点实验室(广西中医药大学), 广西 南宁 530200;
    4.广西高发传染病中西医结合转化医学重点实验室(广西中医药大学), 广西 南宁 530200
  • 收稿日期:2026-01-17 修回日期:2026-04-07 出版日期:2026-11-05 发布日期:2026-11-10
  • 通讯作者: 运晨霞(1977—),女,黑龙江宁安人,广西中医药大学教授。E-mail: yunchenxia@foxmail.com
  • 作者简介:#为共同第一作者
  • 基金资助:
    广西自然科学基金面上项目(2023JJA140940);广西中医药大学高层次人才培育创新团队-医学免疫学创新团队(2022B006);研究生创新创业训练项目(YCSW2023403)

Mechanism of anti-arthritic effect of ethanol extract of Sanye Qingteng on joint injury in adjuvant-induced arthritis rats based on networkpharmacology and molecular docking

Zhao Youwei1#, Hu Mengfan1,2#, Yang Yang1, Liang Liang1,3, Xiao Shundan1, Leng Jing1,3,4, Yun Chenxia1,3*   

  1. 1. School of Basic Medicine, Guangxi University of Chinese Medicine, Nanning Guangxi 530200, China;
    2. Faculty of Chinese Medicine Science, Guangxi University of Chinese Medicine, Nanning Guangxi 530200, China;
    3. GuangxiKey Laboratory of Characteristic Experimental Animal Model,Guangxi University of Chinese Medicine, Nanning Guangxi 530200, China;
    4. Guangxi Key Laboratory ofTranslational Medicine for TreatingHigh Incidence Infectious Diseases with Integrative Medicine,Nanning Guangxi 530200, China
  • Received:2026-01-17 Revised:2026-04-07 Online:2026-11-05 Published:2026-11-10

摘要： 三叶青藤是广西特色壮药,民间常用其泡酒治疗关节肿痛。为探讨三叶青藤醇提物抗佐剂诱导性关节炎(AIA)大鼠关节损伤的作用机制,本文运用网络药理学、分子对接等方法并结合动物实验开展系统性研究。结果表明:三叶青藤醇提物主要成分的作用富集于PI3K/Akt等通路,且主要成分中的甘氨胆酸、大黄素与PI3K/Akt结合能力较强;三叶青藤醇提物干预后AIA大鼠关节肿胀及骨损伤得到改善,破骨细胞数量减少,PI3K/Akt通路蛋白表达下调,MMP-9等骨损伤相关蛋白和基因的表达降低,Ⅱ型胶原蛋白表达升高。研究表明,三叶青藤醇提物可减轻AIA大鼠关节损伤,其机制可能与抑制PI3K/Akt通路、减少破骨细胞数量、下调骨损伤相关蛋白及基因表达有关。

关键词: 三叶青藤, 醇提物, 类风湿关节炎, 骨损伤, 网络药理学, 分子对接, PI3K/Akt

Abstract: Sanye Qingteng is a characteristic Zhuang medicine in Guangxi. In folk medicine, Sanye Qingteng is often used to make wine to treat joint swelling and pain. To explore the effect and mechanism of the ethanol extract of Sanye Qingteng on joint damage in adjuvant-induced arthritis (AIA) rats, this study employed network pharmacology, molecular docking and other methods in combination with animal experiments. The results showed that the main components of the ethanol extract of Sanye Qingteng were enriched in pathways such as PI3K/Akt, and the main components, including glycocholic acid and emodin, had a strong binding ability with PI3K/Akt. After intervention with the ethanol extract of Sanye Qingteng, the joint swelling and bone damage in AIA rats were alleviated, the number of osteoclasts decreased, the expression of PI3K/Akt pathway proteins was down-regulated, the expression of bone damage-related proteins and genes such as MMP-9 was reduced, and the expression of type Ⅱ collagen was increased. The study indicated that the ethanol extract of Sanye Qingteng could alleviate joint damage in AIA rats, and the mechanism might be related to the inhibition of the PI3K/Akt pathway, the reduction of osteoclast numbers, and the down-regulation of bone damage-related proteins and genes.

Key words: Sanye Qingteng, ethanol extract, rheumatoid arthritis, bone injury, network pharmacology, molecular docking, PI3K/Akt

中图分类号:  R593.22;R285.5

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