广西师范大学学报（自然科学版） ›› 2026, Vol. 44 ›› Issue (3): 225-237.doi: 10.16088/j.issn.1001-6600.2025070401

• • 上一篇    

锰离子对硫自养反硝化去除地下水硝态氮的影响机制

农小芳, 江赵洁, 黄雪娇*   

  1. 广西大学 农学院,广西 南宁 530004
  • 收稿日期:2025-07-04 修回日期:2025-09-19 出版日期:2026-05-05 发布日期:2026-05-13
  • 通讯作者: 黄雪娇(1991—),女,广西贵港人,广西大学副教授,博士。E-mail: hxuejiao0412@sina.com
  • 基金资助:
    国家自然科学基金(42107333);中国博士后科学基金(2022M710850);广西科技基地和人才专项(桂科AD23026330)

Mechanism of Divalent Manganese Effect on the Removal of Groundwater Nitrate Nitrogen by Sulfur Autotrophic Denitrification

NONG Xiaofang, JIANG Zhaojie, HUANG Xuejiao*   

  1. College of Agriculture, Guangxi University, Nanning Guangxi 530004, China
  • Received:2025-07-04 Revised:2025-09-19 Online:2026-05-05 Published:2026-05-13

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1

摘要： 水体中的硝态氮污染问题日益严重,威胁人类健康。硫自养反硝化(SADN)技术因其高效、低碳的优点被广泛应用于地下水硝态氮的去除。然而,地下水中常常存在高浓度二价锰离子(Mn2+),其对SADN的影响尚不明确。本研究通过批量实验,采用硫磺(S0)作为电子供体,分析不同Mn2+浓度对SADN性能的影响,并探讨体系中微生物群落结构及氮循环和硫氧化功能基因对Mn2+的响应规律。结果显示,低浓度Mn2+(≤1 mmol/L)显著促进SADN过程,硝态氮去除速率最高达0.735 mmol/(L·d),主导SADN过程的微生物Thiobacillus占比由9.41%最高上升至14.92%,反硝化基因nirS、nosZ和硫氧化基因dsrA、soxB的相对表达量也显著上升。而高浓度Mn2+(>4 mmol/L)则抑制SADN过程,硝态氮去除速率降低至0.674 mmol/(L·d),体系中SADN微生物Thiobacillus和Longilinea占比由23.11%最低降至15.60%,反硝化基因narG、nirS、nirK、norB和硫氧化基因dsrA的表达也受到抑制。综上,Mn2+通过调控SADN体系中功能基因和微生物群落结构进而影响体系的脱氮性能,其中亚硝态氮还原以及S0和硫代硫酸根氧化是不同Mn2+浓度下SADN重要的限速过程。本文从群落结构与功能基因层面揭示Mn2+对SADN过程的响应机制,可为SADN技术在含Mn2+地下水的硝态氮去除应用提供理论依据。

关键词: 二价锰, 硫自养反硝化, 硝态氮, 微生物群落, 功能基因

Abstract: Nitrate nitrogen pollution in water bodies is becoming increasingly severe, posing a threat to human health. Sulphur-autotrophic denitrification (SADN) technology, with its high efficiency and low carbon footprint, has been widely applied for the removal of nitrate nitrogen from groundwater. Groundwater often contains high concentrations of divalent manganese (Mn2+), and the impact of Mn2+ on SADN remains unclear. This study conducted batch experiments using sulfur (S0) as an electron donor to analyse the effects of different Mn2+ concentrations on SADN performance and to investigate the response patterns of microbial community structure, nitrogen cycling, and sulfur oxidation functional genes to Mn2+ in the system. The results showed that low concentrations of Mn2+ (≤1 mmol/L) significantly promoted the SADN process, with the highest nitrate nitrogen removal rate reaching 0.735 mmol/(L·d). The proportion of Thiobacillus, the dominant microorganism in the SADN process, increased from 9.41% to 14.92%, and the relative expression levels of denitrification genes nirS and nosZ, as well as sulfur oxidation genes dsrA and soxB, also significantly increased. However, high concentrations of Mn2+ (>4 mmol/L) inhibited the SADN process, reducing the nitrate nitrogen removal rate to 0.674 mmol/(L·d). The proportion of SADN microorganisms Thiobacillus and Longilinea in the system decreased from 23.11% to 15.60%, and the expression of denitrification genes narG, nirS, nirK, norB, and sulfur oxidation genes dsrA were also inhibited. In summary, Mn2+ affects the denitrification performance of the SADN system by regulating functional genes and microbial community structure in the SADN system, in which nitrite nitrogen reduction as well as S0 and thiosulfate oxidation were important rate-limiting processes in SADN at different Mn2+ concentrations. In this paper, the response mechanism of Mn2+ to the SADN process was revealed at the level of community structure and functional genes, which can provide a theoretical basis for the application of SADN technology in the removal of nitrate nitrogen from Mn2+-containing groundwater.

Key words: divalent manganese, sulfur autotrophic denitrification, nitrate nitrogen, microbial community, functional genes

中图分类号:  X523

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