广西师范大学学报（自然科学版） ›› 2023, Vol. 41 ›› Issue (3): 242-254.doi: 10.16088/j.issn.1001-6600.2022040403

• 研究论文 • 上一篇    

Cr(Ⅵ)浓度对MFC-颗粒污泥耦合体系运行效能及微生态的影响

覃容华1,2, 宿程远1,2*, 陆欣雅2, 陈政鹏2, 周一杰2, 先云川2   

  1. 1.珍稀濒危动植物生态与环境保护教育部重点实验室(广西师范大学), 广西 桂林 541006;
    2.广西师范大学 环境与资源学院, 广西 桂林 541006
  • 收稿日期:2022-04-04 修回日期:2022-05-05 出版日期:2023-05-25 发布日期:2023-06-01
  • 通讯作者: 宿程远(1981—), 男, 广西师范大学教授, 博士。E-mail: suchengyuan2008@126.com
  • 基金资助:
    国家自然科学基金(52060003); 广西师范大学交叉培育项目(2021JC009)

Effects of Cr(Ⅵ) Concentration on the Performance and Microecology of MFC-Granular Sludge Coupling System

QIN Ronghua1,2, SU Chengyuan1,2*, LU Xinya2, CHEN Zhengpeng2, ZHOU Yijie2, XIAN Yunchuan2   

  1. 1. Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection (Guangxi Normal University), Ministry of Education, Guilin Guangxi 541006, China;
    2. School of Environment and Resources, Guangxi Normal University, Guilin Guangxi 541006, China
  • Received:2022-04-04 Revised:2022-05-05 Online:2023-05-25 Published:2023-06-01

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摘要： Cr(Ⅵ)在水体环境中的污染不容忽视,探讨高效处理含Cr(Ⅵ)废水具有重要意义。本文建立了微生物燃料电池(MFC)-颗粒污泥耦合处理含Cr(Ⅵ)废水体系,考察了Cr(Ⅵ)浓度变化对该体系运行效能及微生态的影响。实验结果表明:当进水Cr(Ⅵ)浓度达到80 mg/L时,该体系对COD的去除率与Cr(Ⅵ)生物还原率分别达到92.71%和99.70%;循环伏安法(CV)显示其具有明显的还原峰,电化学阻抗谱(EIS)表明随着进水Cr(Ⅵ)浓度的增加,电荷转移内阻逐渐减小;进水Cr(Ⅵ)浓度增大到100 mg/L时,辅酶F420活性最低为0.026 2±0.000 2 mmol/g,INT-ETS活性明显下降,仅为5.20±0.23 μg/(mg·h);X射线光电子能谱特征峰结合能表明其主要由Cr2O3和Cr(OH)3组成;高通量测序分析表明,该体系中的优势菌群为Chloroflexi和Proteobacteria,进水Cr(Ⅵ)浓度增大至100 mg/L,属水平的优势菌群由以Methanothrix、Methanobacterium等产甲烷菌为主转变为以电活性细菌Geobacter和共养菌属Syntrophobacter为主,其最大相对丰度分别为21.24%、4.28%、5.60%和6.22%,表明增大Cr(Ⅵ)浓度会改变MFC-颗粒污泥耦合体系中的微生物群落结构,Cr(Ⅵ)可通过微生物菌群之间的联营机制进行还原。

关键词: 铬酸盐还原, MFC-颗粒污泥耦合体系, 电化学性能, 微生物群落

Abstract: Heavy metal pollution in water environment cannot be ignored, especially the pollution phenomenon of hexavalent chromium (Cr(Ⅵ)). In order to explore a new idea for efficient treatment of Cr(Ⅵ)-containing wastewater, a microbial fuel cell (MFC)-granular sludge coupling system was established. The effects of the Cr(Ⅵ) concentration on the performance and microecology of MFC-granular sludge coupling system were investigated. When the Cr(Ⅵ) content reached 80 mg/L in the influent, the chemical oxygen demand (COD) degradation and biological reduction efficiency of Cr(Ⅵ) by the system were 92.71% and 99.70%, respectively. In the electrochemical performance analysis, cyclic voltammetry (CV) showed an obvious reduction peak. Meanwhile, the electrochemical impedance spectroscopy (EIS) illustrated that with increasing Cr(Ⅵ) concentrations, the charge transfer resistance gradually decreased. When the influent Cr(Ⅵ) concentration increased to 100 mg/L, the lowest activity of coenzyme F420 was 0.026 2±0.000 2 mmol/g; in addition, the activity of INT-ETS sharply decreased to 5.20±0.23 μg/(mg·h). The morphology of Cr(Ⅵ) after biological reduction of chromate was determined by X-ray photoelectron spectroscopy (XPS), the binding energy of the characteristic peaks demonstrated that was mainly represented by Cr2O3 and Cr(OH)3. High-throughput sequencing analysis illustrated that the dominant microbial flora were Chloroflexi and Proteobacteria. When the concentration of Cr(Ⅵ) increased to 100 mg/L, the dominant microbial community structure were transformed from Methanothrix and Methanobacterium to Geobacter and Syntrophobacter at genus level, the maximum relative abundance of each bacteria were 21.24%, 4.28%, 5.60% and 6.22%, respectively. It was indicated that increasing the concentration of Cr(Ⅵ) would change the microbial community structure of the MFC-granular sludge coupling system, and Cr(Ⅵ) was reduced through the association mechanism between microbial communities.

Key words: chromate reduction, MFC-granular sludge coupling system, electrochemical performance, microbial communities

中图分类号:  X703.1

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