岩黄连总碱诱导肝星状细胞凋亡和自噬的电镜实验研究

doi:10.16088/j.issn.1001-6600.2018.03.011

广西师范大学学报（自然科学版） ›› 2018, Vol. 36 ›› Issue (3): 76-79.doi: 10.16088/j.issn.1001-6600.2018.03.011

岩黄连总碱诱导肝星状细胞凋亡和自噬的电镜实验研究

孟春梅¹, 陆世银², 梁永红², 莫肖敏¹, 李卫东¹, 黄远洁¹, 成晓静¹, 苏志恒², 郑华^{1, *}

1.广西医科大学生命科学研究院,广西南宁530021;
2.广西医科大学药学院,广西南宁530021

收稿日期:2017-06-16 出版日期:2018-07-17 发布日期:2018-07-17
通讯作者: 郑华(1980—),女,广西贵港人,广西医科大学讲师,博士。 E-mail:zhenghua830@aliyun.com
基金资助:
国家自然科学基金地区基金(81560626); 广西自然科学基金面上项目(2013GXNSFAA019161); 广西高校中青年教师基础能力提升项目(KY2016LX046)

Electron Microscopy Study on the Apoptosis and Autophagy of the Hepatic Stellate Cells Induced by Total Alkaloids

MENG Chunmei¹, LU Shiyin², LIANG Yonghong², MO Xiaomin¹, LI Weidong¹, HUANG Yuanjie¹, CHENG Xiaojing¹, SU Zhiheng², ZHENG Hua^{1, *}

1.Life Sciceces Institute,Guangxi Medical University,Nanning Guangxi 530021,China;
2.Pharmaceutical College,Guangxi Medical University,Nanning Guangxi 530021,China

Received:2017-06-16 Online:2018-07-17 Published:2018-07-17

摘要/Abstract

摘要： 为了研究岩黄连总碱诱导肝星状细胞凋亡和自噬的作用,本文通过体外培养HSC-T6细胞,应用MTT法检测不同浓度(0.20、0.25、0.30 g·L^-1)脱氢卡维丁组、巴马汀组和小檗碱组的细胞增殖抑制率,应用透射电子显微镜观察各组HSC-T6细胞的超微结构改变,结果发现:3组不同药物诱导的HSC-T6细胞抑制率均呈浓度依赖性;经不同浓度3组药物处理24 h后的HSC-T6细胞均呈典型的细胞凋亡超微结构改变,并出现不同程度的自噬现象。可见,脱氢卡维丁、巴马汀和小檗碱能诱导HSC-T6细胞凋亡,也能诱导细胞自噬。这3种成分可能为岩黄连总碱中抗肝纤维化的有效成分。

关键词: 凋亡, 自噬, 肝星状细胞, 超微结构

Abstract: To study the effects of total alkaloids on apoptosis and autophagy of hepatic stellate cells in vitro.Cell inhibition rates which using different concentrations (0.20、0.25、0.30 g·L^-1) of Dehydrocavidine group, Pakistan Martin group and Berberine group to the cultured HSC-T6 cells in vitro were detected.The ultrastructural changes of each group cells were observed by transmission electron microscopy. The results showed that, the cell inhibition rates of HSC-T6 cells induced by different drugs in three groups were dependent on concentrations;the HSC-T6 cells showed typical ultrastructural changes of apoptosis after 24h’s processing with different concentrations of the drugs,and there were different levels of the autophagy.Cavitin, Martin and berberine could induce apoptosis and autophagy of the HSC-T6 cells. There three components may be effective to anti liver fibrosis in total alkaloids of the Coptis chinensis Franch.

Key words: apoptosis, autophagy, hepatic stellate cell, ultrastructure

中图分类号:

R962

孟春梅, 陆世银, 梁永红, 莫肖敏, 李卫东, 黄远洁, 成晓静, 苏志恒, 郑华. 岩黄连总碱诱导肝星状细胞凋亡和自噬的电镜实验研究[J]. 广西师范大学学报（自然科学版）, 2018, 36(3): 76-79.

MENG Chunmei, LU Shiyin, LIANG Yonghong, MO Xiaomin, LI Weidong, HUANG Yuanjie, CHENG Xiaojing, SU Zhiheng, ZHENG Hua. Electron Microscopy Study on the Apoptosis and Autophagy of the Hepatic Stellate Cells Induced by Total Alkaloids[J]. Journal of Guangxi Normal University(Natural Science Edition), 2018, 36(3): 76-79.

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岩黄连总碱诱导肝星状细胞凋亡和自噬的电镜实验研究

Electron Microscopy Study on the Apoptosis and Autophagy of the Hepatic Stellate Cells Induced by Total Alkaloids

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