广西师范大学学报（自然科学版） ›› 2023, Vol. 41 ›› Issue (6): 158-168.doi: 10.16088/j.issn.1001-6600.2022123001

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环丙沙星对厌氧反应器处理含磷废水效能及微生物群落响应的影响

杨文静1, 邓钰莲1, 陈铸鑫1, 陶阿凤1, 韦力心1, 吴金燕1, 田翼豪1, 宿程远1,2*   

  1. 1.广西师范大学 环境与资源学院, 广西 桂林 541006;
    2.珍稀濒危动植物生态与环境保护教育部重点实验室(广西师范大学), 广西 桂林 541006
  • 收稿日期:2022-12-30 修回日期:2023-02-13 发布日期:2023-12-04
  • 通讯作者: 宿程远(1981—), 男, 河北晋州人, 广西师范大学教授, 博士。 E-mail: suchengyuan2008@126.com
  • 基金资助:
    国家自然科学基金(52060003); 大学生创新创业训练计划项目(202110602071)

Effects of Ciprofloxacin on Operation Efficiency and Microbial Community Response Characteristics of Anaerobic Reactor for Treatment Phosphorus-Containing Wastewater

YANG Wenjing1, DENG Yulian1, CHEN Zhuxin1, TAO Afeng1, WEI Lixin1, WU Jinyan1, TIAN Yihao1, SU Chengyuan1,2*   

  1. 1. School of Environment and Resources, Guangxi Normal University, Guilin Guangxi 541006, China;
    2. Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection (Guangxi Normal University), Ministry of Education, Guilin Guangxi 541006, China
  • Received:2022-12-30 Revised:2023-02-13 Published:2023-12-04

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摘要： 污水处理中抗生素类污染物的存在可能会对系统中微生物活性产生影响,而微生物活性直接影响系统除COD和除磷的效果。本文研究环丙沙星(CIP)对升流式厌氧污泥床(UASB)处理含磷废水的运行效能及其微生物群落响应的影响, 对COD、 总磷和磷酸盐去除率、 相关酶活性以及微生物群落结构等进行了探讨。 结果表明: 未投加CIP时,UASB反应器对含磷废水COD去除率稳定在98%左右,当CIP增大到10 mg/L时,COD平均去除率下降到63.1%,总磷去除率呈现下降趋势,对磷化氢的浓度影响较大;LB-EPS三维荧光图谱中出现了色氨酸蛋白及辅酶F420有关的特征峰,TB-EPS出现了类腐殖酸的特征峰。在CIP胁迫下,Chloroflexi是细菌中主要的优势菌门,进水CIP浓度为3、5和10 mg/L时,其相对丰度分别为43.13%、44.48%、39.96%;甲烷杆菌属Methanobacterium和甲烷丝菌属Methanothrix是古菌的优势菌属,从而保证了UASB反应器的有效运行。KEGG功能分析表明,古菌与细菌主要以碳水化合物代谢和能量代谢为主,提高进水CIP浓度后,膜运输的丰度降低较大,由10.15%下降到9.31%,且增加CIP浓度对古菌的影响明显。

关键词: 厌氧反应器, 环丙沙星, 除COD, 除磷, 污泥特性, 微生物群落

Abstract: The presence of antibiotic pollutants in wastewater treatment process may affect the microbial activity in sludge, and the microbial activity directly affects the biological phosphorus removal effect of wastewater. Therefore, the effect of ciprofloxacin (CIP) on the operation efficiency of the anaerobic reactor treating high-concentration phosphorus wastewater and the response of microbial community were explored, and the COD removal rate, total phosphorus and phosphate removal rate, related enzyme activity and microbial community structure wereinvestigated. The results showed that the COD removal rate of high-concentration phosphorus wastewater in the reactor was stable at about 98% without CIP, and the average COD removal rate decreased to 63.1% when the CIP concentration was 10 mg/L, and the total phosphorus removal rate showed a downward trend in fluctuations. The addition of 10 mg/L CIP had a great effect on the concentration of phosphine; peaks associated with tryptophan proteins and soluble microbial byproducts and coenzyme F420 appeared in the three-dimensional fluorescence spectra of LB-EPS, and humic acid-like peaks appeared in TB-EPS. Chloroflexi was the dominant phylum under CIP stress, and its relative abundance were 43.13%, 44.48% and 39.96% when the CIP concentration in the influent were 3 mg/L, 5 mg/L and 10 mg/L, respectively. Methanobacterium and Methanothrix were the dominant genera of archaea, thus ensuring the efficient operation of the UASB reactor. KEGG functional analysis showed that archaea and bacteria were mainly carbohydrate metabolism and energy metabolism. After increasing CIP concentration, the functional gene abundance of membrane transport decreased greatly, from 10.15% to 9.31%, indicating that increasing CIP concentration had a greater impact on archaea.

Key words: anaerobic, ciprofloxacin, phosphorus, sludge characteristics, microbial community

中图分类号:  X703.1

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