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Characterization of the Complete Mitochondrial Genome Sequence in Siniperca chuatsi
CHEN Dun-xue, LI Yu-long, BIN Shi-yu, NONG Xiao-xian, LIU Xi-liang, CHENG Jia, ZHANG Jian-she, CHU Wu-ying
Journal of Guangxi Normal University(Natural Science Edition). 2011, 29 (4):
111-116.
The complete mitochondrial DNA (mtDNA) of Sinipercachuatsi was cloned and sequenced.The mitochondrial genomes is 16 496 bp in length and contains 37 genes (13 protein-coding genes,2 ribosomal RNA,and 22 transfer tRNAs) and a major non-coding region.There are only 32 bp nucleotides gapand 35 bp nucleotides overlap in the mitochondrial genome.The mitochondrialgenome has a better A+T bias and the G content is relatively low.Except for cytochrome coxidase subunit 1 (CO1),which began with GTG as an initiation codon,allproteins started with an ATG codon.However,the stop codons of the mitochondrialgenomes examined differed from the initiation codons with variety.The tRNA-leund tRNA-Ser are double style genes.The three tRNA clusters-IQM,WANCY,and HSL whose base composition is very similar to those reported in bony fish.The 12SrRNAand the 16SrRNA genes,as in other bony fishes,were located between the tRNA-Pheand tRNA-Leu(UUR),and were separated by the tRNA-Val.The control region was observed to have a better A+T bias.These results were helpful for further understanding the phylogenetic classification and evolution of sinipercine fishes.Genetic structure of the three Siniperca fishes could be used as a basis for itsgenetic conversation and utilization.
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