Abstract: The complete mitochondrial DNA (mtDNA) of Sinipercachuatsi was cloned and sequenced.The mitochondrial genomes is 16 496 bp in length and contains 37 genes (13 protein-coding genes,2 ribosomal RNA,and 22 transfer tRNAs) and a major non-coding region.There are only 32 bp nucleotides gapand 35 bp nucleotides overlap in the mitochondrial genome.The mitochondrialgenome has a better A+T bias and the G content is relatively low.Except for cytochrome coxidase subunit 1 (CO1),which began with GTG as an initiation codon,allproteins started with an ATG codon.However,the stop codons of the mitochondrialgenomes examined differed from the initiation codons with variety.The tRNA-leund tRNA-Ser are double style genes.The three tRNA clusters-IQM,WANCY,and HSL whose base composition is very similar to those reported in bony fish.The 12SrRNAand the 16SrRNA genes,as in other bony fishes,were located between the tRNA-Pheand tRNA-Leu(UUR),and were separated by the tRNA-Val.The control region was observed to have a better A+T bias.These results were helpful for further understanding the phylogenetic classification and evolution of sinipercine fishes.Genetic structure of the three Siniperca fishes could be used as a basis for itsgenetic conversation and utilization.

Key words: Siniperca chuatsi, mitochondrial genome, codon bias, varity, tRNA clusters