Journal of Guangxi Normal University(Natural Science Edition) ›› 2011, Vol. 29 ›› Issue (4): 104-110.

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Cloning Glucose-flavonoid 3-o-glucosyltransferase (UFGT) from Litchi and Expression in Escherichia coli

ZHAO Zhi-chang1,2, HU Fu-chu1,2, HU Gui-bing1,2, WANG Hui-cong1,3, YANG Zhuan-ying1,3, SU Chun-lan1,2, LI Jia-qiang1,2   

  1. 1.Laboratory for Fruit Resource and Cultivar Improvement,Guangzhou Guangdong 510642,China;
    2.Institute of Biotechnology for Horticultural Plants,Guangzhou Guangdong510642,China;
    3.Physiological Laboratory for South China Fruits,College of Horticulture,South China Agricultural University,Guangzhou Guangdong 510642,China
  • Received:2011-06-10 Published:2018-11-16

Abstract: Litchi is native to South China's tropical fruit trees,the fruit color is various types.Function of Glucose-flavonoid 3-o-glucosyltransferase (UFGT) is catalyzed the unstable anthocyanidin into anthocyanin.Itis the last enzyme in anthocyanin biosynthetic pathway.Within a certain range,UFGT activity is positively related to anthocyanin synthesis,and enzyme activity of UFGT inhibited has more influence on anthocyanin synthesis than other enzymes.Many research results show that UFGT is a key enzyme gene in anthocyanin synthesis.This study is based on the known UFGT gene fragments in the Litchi,the full-length cDNA of UFGT was cloned using the 3′RACE and 5′RACE methods.It was found an intron in the genomic DNA sequence compared to cDNA sequence.The cDNA fragment of UFGT directionally cloned into the vector pET32a,the UFGTfusion protein about 60 KDa was expressed by induction 1 mmol/L IPTG in E.coli.

Key words: Litchi chinensis Sonn., anthocyanin, UFGT, gene cloning, prokaryotic expression

CLC Number: 

  • S667.1
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