Journal of Guangxi Normal University(Natural Science Edition) ›› 2018, Vol. 36 ›› Issue (2): 141-147.doi: 10.16088/j.issn.1001-6600.2018.02.020

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Cloning and Expression Analysis of Ubiquitin-Specific Protease 7 Gene under Starvation in Mandarin Fish (Siniperca chuatsi)

ZHANG Fangliang1,DENG Xiaohong2,ZHU Xiaotong1,XU Lei1,HU Jiangli1,BIN Shiyu1*   

  1. 1. College of Life Science, Guangxi Normal University, Guilin Guangxi 541006,China;
    2.Quanzhou Fisheries Technology Station, Guilin Guangxi 541500,China
  • Received:2017-05-10 Online:2018-05-10 Published:2018-07-18

Abstract: To investigate the effect of starvation on the expression of USP7 gene in the liver of Mandarin fish, the full-length cDNA sequence of the USP7 gene was cloned from the liver tissue of Mandarin fish by 3′-RACE and 5′-RACE techniques (Accession No. MF000683) with 4 319 bp and its expression profile upon starvation was assayed by real-time quantitative PCR. Bioinformatics analysis showed that the full-length coding region of USP7 gene is 3 336 bp with 1 111 encoded amino acids. Among them, 180 and 137 ones are negatively and positively charged, respectively. The relative expression of USP7 gene in the liver at different starvation time showed a peak change, which had a significant increase and reached the highest value after a starvation duration of 4 days, then decreased gradually after a starvation duration of 7 days and finally reached the initial level (P<0.05). Our studies provided novel information about the USP7gene and its expression status upon starvation.

Key words: Mandarin fish(Siniperca chuatsi), USP7 gene, liver, expression analysis

CLC Number: 

  • Q959.483
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