Journal of Guangxi Normal University(Natural Science Edition) ›› 2020, Vol. 38 ›› Issue (3): 104-109.doi: 10.16088/j.issn.1001-6600.2020.03.013

Previous Articles     Next Articles

Growth and Effects of High-fat Diet Feed on Blood Physiological and Biochemical Indicators of Sdr9c7+/- Mice

ZENG Zhipeng1,2, ZHOU Jie1,2, LU Weimin3, CHEN Qiaoyuan1,2, HE Sinuo1,2, LIN Wanhua1,2*   

  1. 1. Guangxi Universities Key Laboratory of Stem Cell and Biopharmaceutical Technology (Guangxi Normal University), Guilin Guangxi 541006, China;
    2. College of Life Sciences, Guangxi Normal University, Guilin Guangxi 541006, China;
    3. Affiliated Hospital, Guangxi Normal University, Guilin Guangxi 541006, China
  • Received:2019-03-26 Online:2020-05-25 Published:2020-06-11

Abstract: Wild type (Sdr9c7+/+) and heterozygote type (Sdr9c7+/-) male mice were used to analyze the effects of Sdr9c7 gene on the growth and development under regular chow diets and high-fat diets, and the physiological and biochemical indicators of blood and liver steatosis of mice under high-fat diets were also measured. Results: (1) Compared with Sdr9c7+/+ mice, Sdr9c7+/- mice developed normally, and there was no significant difference in their body weight curves. (2) When the mice fed with high-fat diet to 15 weeks of age, there was no difference in the level of blood glucose (GLU), serum total cholesterol (TCHO), triglyceride (TG), low-density cholesterol (LDL), alanine aminotransferase (ALT), alanine aminotransferase (AST), urea (BUN) and creatinine (CREA) of the mice between the two groups. At the age of 28 weeks under high-fat diets, the AST and ALT levels in the heterozygote group were significantly increased (P<0.05), suggesting that Sdr9c7 might be associated with hepatitis, but there was no significant difference in liver coefficient and cell vacuole area of paraffin section, indicating that Sdr9c7 gene had no effect on hepatic steatosis.

Key words: Sdr9c7 gene, growth and development, high-fat feed, blood physiological and biochemical indicators, fatty liver

CLC Number: 

  • Q592.1
[1] PERSSON B, KALLBERG Y. Classification and nomenclature of the superfamily of short-chain dehydrogenases/reductases (SDRs)[J]. Chemico-biological Interactions, 2013,202(1/2/3):111-115.
[2] OPPERMANN U C, SALIM S, TJERNBERG L O, et al. Binding of amyloid beta-peptide to mitochondrial hydroxyacyl-CoA dehydrogenase (ERAB): regulation of an SDR enzyme activity with implications for apoptosis in Alzheimer's disease[J]. FEBS Letters, 1999,451(3):238-242.
[3] CHANG N S, SCHULTZ L, HSU L J, et al. 17β-Estradiol upregulates and activates WOX1/WWOXv1 and WOX2/WWOXv2 in vitro: potential role in cancerous progression of breast and prostate to a premetastatic state in vivo[J]. Oncogene, 2005,24(4):714-723.
[4] STEWART P M, BOULTON A, KUMAR S, et al. Cortisol metabolism in human obesity: impaired cortisone→ cortisol conversion in subjects with central adiposity[J]. The Journal of Clinical Endocrinology and Metabolism, 1999,84(3):1022-1027.
[5] 汤善宏. 基于表达谱芯片技术的食管癌转移相关基因筛选及功能鉴定[D]. 西安: 第四军医大学, 2013.
[6] CHEN W, SONG M S, NAPOLI J L. SDR-O: an orphan short-chain dehydrogenase/reductase localized at mouse chromosome 10/human chromosome 12[J]. Gene, 2002,294(1/2):141-146.
[7] TAKEICHI T, NOMURA T, TAKAMA H, et al. Deficient stratum corneum intercellular lipid in a Japanese patient with lamellar ichthyosis with a homozygous deletion mutation in SDR9C7[J]. The British Journal of Dermatology, 2017,177(3):e62-e64.
[8] LINDHOLM-PERRY A K, CUNNINGHAM H C, KUEHN L A, et al. Relationships between the genes expressed in the mesenteric adipose tissue of beef cattle and feed intake and gain[J]. Animal Genetics, 2017,48(4):386-394.
[9] MARCHESINI G, BUGIANESI E, FORLANI G, et al. Nonalcoholic fatty liver, steatohepatitis, and the metabolic syndrome[J]. Hepatology (Baltimore, Md), 2003,37(4):917-923.
[10]李卫娟. 多种生化指标检验用于脂肪肝临床诊断的价值评定[J]. 中国实用医药, 2018,13(21):64-65.
[11]SHIGEHARA Y, OKUDA S, NEMER G, et al. Mutations in SDR9C7 gene encoding an enzyme for vitamin A metabolism underlie autosomal recessive congenital ichthyosis[J]. Human Molecular Genetics, 2016,25(20):4484-4493.
[12]HOTZ A, BOURRAT E, KÜSEL J, et al. Mutation update for CYP4F22 variants associated with autosomal recessive congenital ichthyosis[J]. Human Mutation, 2018,39(10):1305-1313.
[1] TUO Wan-liang, PENG Feng-lin. Effects on Lipid Metabolism,Antioxidant Capacity and Non-Alcoholic Fatty Liver Disease Formation in Rats with High Fat Diet [J]. Journal of Guangxi Normal University(Natural Science Edition), 2015, 33(4): 144-151.
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed   
[1] XU Lun-hui, LIU Jing-ning, ZHU Qun-qiang, WANG Qing, XIE Yan, SUO Sheng-chao. Path Deviation Control of Automatic Guided Vehicle[J]. Journal of Guangxi Normal University(Natural Science Edition), 2015, 33(1): 1 -6 .
[2] KUANG Xian-yan, WU Yun, CAO Wei-hua, WU Yin-feng. Cellular Automata Simulation Model for Urban MixedNon-motor Vehicle Flow[J]. Journal of Guangxi Normal University(Natural Science Edition), 2015, 33(1): 7 -14 .
[3] XIAO Rui-jie, LIU Ye, XIU Xiao-ming, KONG Ling-jiang. State Transfer of Two Mechanical Oscillators in Coupled CavityOptomechanical System[J]. Journal of Guangxi Normal University(Natural Science Edition), 2015, 33(1): 15 -19 .
[4] HUANG Hui-qiong, QIN Yun-mei. Overtaking Model Based on Drivers’ Characteristics[J]. Journal of Guangxi Normal University(Natural Science Edition), 2015, 33(1): 20 -26 .
[5] YUAN Le-ping, SUN Rui-shan. Probabilistic Safety Assessment of Air Traffic Conflict Resolution[J]. Journal of Guangxi Normal University(Natural Science Edition), 2015, 33(1): 27 -31 .
[6] YANG Pan-pan, ZHU Long-ji, CAO Meng-jie. TSC Type of Reactive Power Compensation Control SystemBased on STM32[J]. Journal of Guangxi Normal University(Natural Science Edition), 2015, 33(1): 32 -37 .
[7] ZHANG Mei-yue. Some New Results for the Electron Beams Focusing System Model[J]. Journal of Guangxi Normal University(Natural Science Edition), 2015, 33(1): 38 -44 .
[8] HOU Xiao-dong, CAI Bin-bin, JIN Wei-dong, DUAN Wang-wang. A New Weighted Evidence Fusion Algorithm Based on Evidence Distanceand Fuzzy Entropy Theory[J]. Journal of Guangxi Normal University(Natural Science Edition), 2015, 33(1): 45 -51 .
[9] YUE Cai-jie, CHEN Yuan-yan, ZHU Xin-hua. An Effective Area Query Algorithm in Sensor Network[J]. Journal of Guangxi Normal University(Natural Science Edition), 2015, 33(1): 52 -58 .
[10] XU Shang-Jin, LI Ping-shan, HUANG Hai-hua, LI Jing-jian. 8-Valent 1-Regular Cayley Graphs WhoseVertex Stabilizer is Z4×Z2[J]. Journal of Guangxi Normal University(Natural Science Edition), 2015, 33(1): 59 -66 .