Journal of Guangxi Normal University(Natural Science Edition) ›› 2011, Vol. 29 ›› Issue (4): 88-91.

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Denaturation Study of Bovine Hemoglobin Induced by Guanidine Chloride by Microcalorimetry

LI Xiang-rong1,2, YU Jie1, HAO Xiao-xiao2   

  1. 1.School of Basic Medicine,Xinxiang Medical University,Xinxiang Henan 453003,China;
    2.College of Environmental Science,Henan Normal University,Xinxiang Henan453007,China
  • Received:2011-06-16 Published:2018-11-16

Abstract: The interactions between guanidine chloride and bovine hemoglobin in aqueous solution as well as the denaturation process of bovine hemoglobin were studied by using isothermal microcalorimetry methodat 30 °C and three pHs.The simple binding model was employed to obtain the binding constants and the binding free energies.Furthermore,the linear extrapolationat the midpoint of transition was employed to determine the apparent denaturation enthalpy.The results showed that the interactions between guanidine chloride andbovine hemoglobin is divided into three stages.The apparent denaturation enthalpy of bovine hemoglobin by guanidine chloride is 200 kJ·mol-1 at pH5.21 and 540 kJ·mol-1 at pH6.70,while it is 120 kJ·mol-1 at pH8.03.BHbis most stable at its isoelectric pH.

Key words: bovine hemoglobin, isothermal microcalorimetry, guanidine chloride, denaturation

CLC Number: 

  • O642.3
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