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Component Analysis and Content Determination of Characteristic Flavonoids in Ginkgo biloba Extract and Its Injections
HU Qingfeng, RAN Qingnian, LIU Jie, WANG Jinghe, HUI Ailing, ZHANG Wencheng
Journal of Guangxi Normal University(Natural Science Edition). 2025, 43 (2):
238-246.
DOI: 10.16088/j.issn.1001-6600.2024032606
To analyze the characteristic flavonoids in Ginkgo biloba extract (GBE) and its injections, the HPLC fingerprint and liquid chromatography-mass spectrometry (LC-MS) were used to determine the flavonoid components. Additionally, a HPLC quantitative detection method for determining the total flavonoids content was established. Eight flavonoids including rutin, isoquercetin, kaempferol-3-O-rutinoside, narcissus, astragalin, quercetin, kaempferol and isorhamnetin occupied the high content in GBE and injections, which served as the characteristic flavonoid components for further research. The HPLC quantitative detection was performed using a C18 column, acetonitrile-0.2% phosphoric acid as gradient elution, flow rate of 1.0 mL/min, detection wavelength of 360 nm, and column temperature of 30 ℃. Eight flavonoids demonstrated good linear relationship (r > 0.999) when their concentration ranged from 6.25 to 75.00, 1.00 to 12.00, 1.75 to 21.00, 1.75 to 21.00, 1.25 to 15.00, 1.25 to 15.00, 0.50 to 6.00 and 0.50 to 6.00 mg/L, respectively. The precision, repeatability, stability, and recovery rate RSD were less than 2%. The total flavonoid content in three GBEs were 61.37,76.23,140.55 mg/g, respectively. Also, four injections gave the results of 246.0, 305.2, 284.5, 250.8 mg/L. This study identifies eight characteristic flavonoids in GBE and its injections and establishes a quantitative detection method, which will provide an important reference for the quality monitoring of GBE preparations.
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