嗜盐真菌Cladosporium cladosporioides GXIMD 00533次级代谢产物及其生物活性研究

doi:10.16088/j.issn.1001-6600.2024082203

Abstract

Abstract: The secondary metabolites of Cladosporium cladosporioides GXIMD 00533 from Guangxi Beihai Zhulin Salt Field, were studied by using high salinity medium. The fungal extract was separated and purified by silica gel, reversed phase ODS column chromatography and semi-preparative high performance liquid chromatography (HPLC) to obtain 16 compounds. Their structures were elucidated by NMR spectral data and identified as four lactone compounds: iso-cladospolide B (1), cladospolide B (2), pandangolide 1 (3), thiocladospolide F (4), two sterol compounds: cladosporisteroid B (5) and (22E,24R)-3β,5α,9α-trihydroxyergosta-7,22-dien-6-one (6), seven diketopiperazine: brevianamide F (7), N-acetyl-L-phenylalanine (8), cyclo (L-Pro-L-Leu) (9), cyclo (L-Ala-L-Leu) (10), cyclo (L-Val-L-Ala) (11), cyclo (L-Gly-L-Ile) (12), cyclo (L-Leu-L-Gly) (13), one amino acid: L-tryptophan (14), and two fatty acids: cladosporacid B (15) and (4R,5S,11R,2E)-4,5,11-trihydroxy-2-dodecenoate (16). The antioxidant, antibacterial, α-Glucosidase inhibitory, cytotoxic activities, anti-barnala adhesion and acetylcholinesterase inhibitory activities of all compounds were evaluated. Compounds 6 and 14 had significant iron ion reduction and antioxidant capacity. Compound 6 exhibited inhibitory activities on α-glucosidase with IC₅₀ of 2.662 μmol·L^-1; Compound 4 had significant anti-barnacle adhesion activity, and the anti-adhesion rate was 100%. Some of the compounds showed certain acetylcholinesterase activity, and the inhibitory rate of compound 5 reached 54.98%.

Key words: marine salt field, halophilic fungus, Cladosporium, secondary metabolite, marine fungus

CLC Number: Q936; TQ28; P745

FU Chunqing, LIANG Chunxiang, LIANG Lifen, GAO Chenghai, LIU Yonghong, XU Xinya. Secondary Metabolites and Biological Activities of Halophilic Fungus Cladosporium cladosporioides GXIMD 00533[J].Journal of Guangxi Normal University(Natural Science Edition), 2025, 43(2): 247-257.

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Secondary Metabolites and Biological Activities of Halophilic Fungus Cladosporium cladosporioides GXIMD 00533

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