Journal of Guangxi Normal University(Natural Science Edition) ›› 2023, Vol. 41 ›› Issue (4): 208-219.doi: 10.16088/j.issn.1001-6600.2022062701

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A Method to Rapidly Construct a Mouse Obesity Model

HUANG Li1,2, LIU Zebiao1,2, ZHU Yu1,2, WANG Tao1,2, WU Qiong1,2*   

  1. 1. Guangxi Universities Key Laboratory of Stem Cell and Biopharmaceutical Technology (Guangxi Normal University), Guilin Guangxi 541004, China;
    2. College of Life Sciences, Guangxi Normal University, Guilin Guangxi 541006, China
  • Received:2022-06-27 Revised:2022-10-07 Online:2023-07-25 Published:2023-09-06

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Abstract: High-fat diet was used to construct a mouse obesity model, and it was found that the traditional high-fat diet had problems such as poor palatability, long modeling time, and low molding rate. In response to these problems, the obesity model of C57BL/6J mice was established by improving the traditional high-fat diet formula and using the method of interval feeding. After modeling, serum biochemical indexes, fat weight, and histopathological changes of liver, fat and pancreas were detected. The results showed that: 1) feeding the improved high-fat diet could shorten the modeling time, and the mouse obesity model could be established in only 6 weeks under the interval feeding method; 2) the serum liver function and renal function of the high-fat diet group related to Biochemical indicators were normal, blood lipids was elevated (P<0.01), and histopathological tissue sections showed mild to moderate edema in liver tissue, increased adipocyte volume, and no abnormality in pancreatic tissue. The improved high-fat feed in this experiment had high palatability and could increase the body weight of mice in a short time, which was shorter than the same type of high-fat feed on the market and had food safety.

Key words: high-fat diet feeding, diet-induces obesity, obesity model, high-fat diet, C57BL/6J mice

CLC Number:  R589.2; R-332
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