Journal of Guangxi Normal University(Natural Science Edition) ›› 2023, Vol. 41 ›› Issue (4): 220-230.doi: 10.16088/j.issn.1001-6600.2022070602

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Effect of Dual Mixed Culture of Endophytic Bacteria from Tripterygium wilfordii on Production of Plant Growth Promoting Substances

SUN Rui1, SONG Ping1,2*, LIU Jie1, HONG Wei1,2, WU Chengzhen2,3, FENG Lei4   

  1. 1. College of Forestry, Fujian Agriculture and Forestry University, Fuzhou Fujian 350002, China;
    2. Fujian Provincial Key Laboratory of Forest Ecosystem Processing and Management (Fujian Agriculture and Forestry University), Fuzhou Fujian 350002, China;
    3. Wuyi University, Wuyishan Fujian 354300, China;
    4. College of Resources and Environment, Fujian Agriculture and Forestry University, Fuzhou Fujian 350002, China
  • Received:2022-07-06 Revised:2022-07-28 Online:2023-07-25 Published:2023-09-06

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Abstract: In this study, 23 endophytic bacteria isolated from the medicinal plant Tripterygium wilfordii were applied as experimental strains to determine the production of IAA, ACC deaminase and siderophore and phosphate solubilization capacity in the monoculture and dual mixed culture (253 combinations). By comparison, the effects of coexistence and interactions of endophytic bacteria on growth-promoting substance production were evaluated. The results indicated that interactions between co-cultured bacteria obviously changed the production of various growth-promoting substances. Compared with the monoculture of strains, abilities of phosphate solubilization and IAA secretion in most of the mixed culture had great improvement. The strains that were co-cultured with LY4 were found to have larger increment of phosphate solubilization. The strain mixtures including LG3, LJ3 or LJ12 showed higher secretion of IAA. Higher ACC deaminase activity were tested from the strain mixtures including LJ12. The strains LJ12 and LY3 had important contribution to siderophore production of their strain mixtures. The growth-promoting index of the mixtures of most of the strains showed no significant correlation to those of the single strains. The growth-promoting properties of the mixtures of the endophytic bacteria did not depend on the abilities of the single strains.

Key words: Tripterygium wilfordii, endophytic bacteria, mixed culture, growth-promoting

CLC Number:  S567.19; Q939.96
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