Journal of Guangxi Normal University(Natural Science Edition) ›› 2023, Vol. 41 ›› Issue (4): 220-230.doi: 10.16088/j.issn.1001-6600.2022070602

Previous Articles     Next Articles

Effect of Dual Mixed Culture of Endophytic Bacteria from Tripterygium wilfordii on Production of Plant Growth Promoting Substances

SUN Rui1, SONG Ping1,2*, LIU Jie1, HONG Wei1,2, WU Chengzhen2,3, FENG Lei4   

  1. 1. College of Forestry, Fujian Agriculture and Forestry University, Fuzhou Fujian 350002, China;
    2. Fujian Provincial Key Laboratory of Forest Ecosystem Processing and Management (Fujian Agriculture and Forestry University), Fuzhou Fujian 350002, China;
    3. Wuyi University, Wuyishan Fujian 354300, China;
    4. College of Resources and Environment, Fujian Agriculture and Forestry University, Fuzhou Fujian 350002, China
  • Received:2022-07-06 Revised:2022-07-28 Online:2023-07-25 Published:2023-09-06

PDF (PC)

172

Abstract: In this study, 23 endophytic bacteria isolated from the medicinal plant Tripterygium wilfordii were applied as experimental strains to determine the production of IAA, ACC deaminase and siderophore and phosphate solubilization capacity in the monoculture and dual mixed culture (253 combinations). By comparison, the effects of coexistence and interactions of endophytic bacteria on growth-promoting substance production were evaluated. The results indicated that interactions between co-cultured bacteria obviously changed the production of various growth-promoting substances. Compared with the monoculture of strains, abilities of phosphate solubilization and IAA secretion in most of the mixed culture had great improvement. The strains that were co-cultured with LY4 were found to have larger increment of phosphate solubilization. The strain mixtures including LG3, LJ3 or LJ12 showed higher secretion of IAA. Higher ACC deaminase activity were tested from the strain mixtures including LJ12. The strains LJ12 and LY3 had important contribution to siderophore production of their strain mixtures. The growth-promoting index of the mixtures of most of the strains showed no significant correlation to those of the single strains. The growth-promoting properties of the mixtures of the endophytic bacteria did not depend on the abilities of the single strains.

Key words: Tripterygium wilfordii, endophytic bacteria, mixed culture, growth-promoting

CLC Number:  S567.19; Q939.96
[1] 邹文欣, 谭仁祥. 植物内生菌研究新进展[J]. 植物学报, 2001, 43(9): 881-892. DOI: 10.3321/j.issn:1672-9072.2001.09.001.
[2] 白建飞, 韩升才, 高聚林, 等. 内生菌莫拉维假单胞菌GF-55促进玉米生长和提高抗倒伏功能分析[J].微生物学通报, 2022, 49(7): 2625-2637. DOI: 10.13344/j.microbiol.china.211049.
[3] 刘文韬, 朱雪竹, 陈雪梅, 等. 功能内生菌株Enterobacter sp. PRd5定殖降低农作物芘污染技术研究[J]. 南京农业大学学报, 2020, 43(2): 274-283. DOI: 10.7685/jnau.201904048.
[4] 许进娇, 宋萍, 封磊, 等. 雷公藤内生细菌的促生作用及其对雷公藤甲素生成的影响[J]. 应用生态学报, 2014, 25(6):1681-1687. DOI: 10.13287/j.1001-9332.20140409.016.
[5] WAQAS M, KHAN A L, LEE I J. Bioactive chemical constituents produced by endophytes and effects on rice plant growth[J]. Journal of Plant Interactions, 2014, 9(1): 478-487. DOI: 10.1080/17429145.2013.860562.
[6] WANG X M, YAM T W, MENG Q W, et al. The dual inoculation of endophytic fungi and bacteria promotes seedlings growth in Dendrobium catenatum (Orchidaceae) under in vitro culture conditions[J]. Plant Cell, Tissue and Organ Culture(PCTOC), 2016, 126(3): 523-531. DOI: 10.1007/s11240-016-1021-6.
[7] DO NASCIMENTO J S, SILVA F M, MAGALLANES-NOGUERA C A, et al. Natural trypanocidal product produced by endophytic fungi through co-culturing[J]. Folia Microbiologica, 2020, 65(2): 323-328. DOI: 10.1007/s12223-019-00727-x.
[8] KARUPPIAH V, VALLIKKANNU M, LI T T, et al. Simultaneous and sequential based co-fermentations of Trichoderma asperellum GDFS1009 and Bacillus amyloliquefaciens 1841: a strategy to enhance the gene expression and metabolites to improve the bio-control and plant growth promoting activity[J]. Microbial Cell Factories, 2019, 18(1):185-185. DOI: 10.1186/s12934-019-1233-7.
[9] BRIC J M, BOSTOCK R M, SILVERSTONE S E. Rapid in situ assay for indoleacetic acid production by bacteria immobilized on a nitrocellulose membrane[J]. Applied and Environmental Microbiology, 1991, 57(2): 535-538. DOI: 10.1128/aem.57.2.535-538.1991.
[10] HONMA M, SHIMOMURA T. Metabolism of 1-aminocyclopropane-1-carboxylic acid[J]. Agricultural and Biological Chemistry, 1978, 42(10): 1825-1831. DOI: 10.1080/00021369.1978.10863261.
[11] SCHWYN B, NEILANDS J B. Universal chemical assay for the detection and determination of siderophores[J]. Analytical Biochemistry, 1987, 160(1): 47-56. DOI: 10.1016/0003-2697(87)90612-9.
[12] LUO J, ZHOU J J, ZHANG J Z. Aux/IAA gene family in plants: molecular structure, regulation, and function[J]. International Journal of Molecular Sciences, 2018, 19(1): 259. DOI: 10.3390/ijms19010259.
[13] 狄义宁, 谢林艳, 谷书杰, 等. 甘蔗及甘蔗近缘属内生菌的筛选、鉴定与功能研究[J]. 中国农业大学学报, 2021, 26(11): 70-83. DOI: 10.11841/j.issn.1007-4333.2021.11.07.
[14] 赵龙飞, 徐亚军, 邓振山, 等. 拮抗棉花枯萎病菌的地黄内生细菌筛选、鉴定和促生潜能[J]. 微生物学报, 2021, 61(8): 2338-2357. DOI: 10.13343/j.cnki.wsxb.20200504.
[15] SOFY M R, ABOSEIDAH A A, HENEIDAK S A, et al. ACC deaminase containing endophytic bacteria ameliorate salt stress in Pisum sativum through reduced oxidative damage and induction of antioxidative defense systems[J]. Environmental Science and Pollution Research Internationlal, 2021, 28(30): 40971-40991. DOI: 10.1007/s11356-021-13585-3.
[16] RADWAN T E E, MOHAMED Z K, REIS V M. Production of indole-3-acetic acid by different strains of Azospirillum and Herbaspirillum spp.[J]. Symbiosis, 2002, 32(1): 39-54. DOI: 10.1634/stemcells.20-6-585.
[17] GUPTA S, PANDEY S. ACC deaminase producing bacteria with multifarious plant growth promoting traits alleviates salinity stress in French bean (Phaseolus vulgaris) plants[J]. Frontiers in Microbiolology, 2019, 10:1506. DOI: 10.3389/fmicb.2019.01506.
[18] 黄静, 盛下放, 何琳燕. 具溶磷能力的植物内生促生细菌的分离筛选及其生物多样性[J]. 微生物学报, 2010, 50(6): 710-716. DOI: 10.13343/j.cnki.wsxb.2010.06.002.
[19] LI Z, BAI T S, DAI L T, et al. A study of organic acid production in contrasts between two phosphate solubilizing fungi: Penicillium oxalicum and Aspergillus niger[J]. Scientific Reports, 2016, 6: 25313. DOI: 10.1038/srep25313.
[20] 冯哲叶, 陈莎莎, 王文超, 等. 几株溶磷细菌的筛选和鉴定及其溶磷效果[J]. 南京农业大学学报, 2017, 40(5): 842-849. DOI: 10.7685/jnau.201612008.
[21] VANDERSTRAETEN L, VAN DER STRAETEN D. Accumulation and transport of 1-Aminocyclopropane-1-Carboxylic acid (ACC) in plants: current status, considerations for future research and agronomic applications[J]. Frontiers in Plant Science, 2017, 8: 38. DOI: 10.3389/fpls.2017.00038.
[22] ISLAM M R, MADHAIYAN M, DEKA BORUAH H P, et al. Characterization of plant growth-promoting traits of free-living diazotrophic bacteria and their inoculation effects on growth and nitrogen uptake of crop plants[J]. Journal of Microbiology and Biotechnology, 2009, 19(10): 1213-1222. DOI: 10.4014/jmb.0903.03028.
[23] RASHID S, CHARLES T C, GLICK B R. Isolation and characterization of new plant growth-promoting bacterial endophytes[J]. Applied Soil Ecology, 2012, 61: 217-224. DOI: 10.1016/j.apsoil.2011.09.011.
[24] SARAPAT S, LONGTONGLANG A, UMNAJKITIKORN K, et al. Application of rice endophytic Bradyrhizobium strain SUTN9-2 containing modified ACC deaminase to rice cultivation under water deficit conditions[J]. Journal of Plant Interactions, 2020, 15(1): 322-334. DOI: 10.1080/17429145.2020.1824028.
[25] CAO L, GAO Y, YU J, et al. Streptomyces hygroscopicus OsiSh-2-induced mitigation of Fe deficiency in rice plants[J]. Plant Physiology and Biochemistry, 2021, 158: 275-283. DOI: 10.1016/j.plaphy.2020.11.013.
[26] ZENG J R, XU T, CAO L D, et al. The role of iron competition in the antagonistic action of the rice endophyte Streptomyces sporocinereus OsiSh-2 against the pathogen Magnaporthe oryzae[J]. Microbial Ecology, 2018, 76(4): 1021-1029. DOI: 10.1007/s00248-018-1189-x.
[27] KRAMER J, ÖZKAYA Ö, KÜMMERLI R. Bacterial siderophores in community and host interactions[J]. Nature Reviews Microbiology, 2020, 18(3): 152-163. DOI: 10.1038/s41579-019-0284-4.
[28] TIWARI P, BAE H H. Horizontal gene transfer and endophytes: an implication for the acquisition of novel traits[J]. Plants (Basel, Switzerland), 2020, 9(3): 305. DOI: 10.3390/plants9030305.
[29] GU Y F, WANG Y Y, SUN Y H, et al. Genetic diversity and characterization of arsenic-resistant endophytic bacteria isolated from Pteris vittata, an arsenic hyperaccumulator[J]. BMC Microbiology, 2018, 18(1): 42. DOI: 10.1186/s12866-018-1184-x.
[30] WANG Y J, LI H, ZHAO W, et al. Induction of toluene degradation and growth promotion in corn and wheat by horizontal gene transfer within endophytic bacteria[J]. Soil Biology and Biochemistry, 2010, 42(7): 1051-1057. DOI: 10.1016/j.soilbio.2010.03.002.
No related articles found!
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
[1] XU Jiu-cheng, LI Xiao-yan, LI Shuang-qun, ZHANG Ling-jun. Feature Images Retrieval Method of Tolerance Granular-basedMulti-level Texture[J]. Journal of Guangxi Normal University(Natural Science Edition), 2011, 29(1): 186 -187 .
[2] BAI Defa, XU Xin, WANG Guochang. Review of Generalized Linear Models and Classification for Functional Data[J]. Journal of Guangxi Normal University(Natural Science Edition), 2022, 40(1): 15 -29 .
[3] ZENG Qingfan, QIN Yongsong, LI Yufang. Empirical Likelihood Inference for a Class of Spatial Panel Data Models[J]. Journal of Guangxi Normal University(Natural Science Edition), 2022, 40(1): 30 -42 .
[4] ZHANG Xilong, HAN Meng, CHEN Zhiqiang, WU Hongxin, LI Muhang. Survey of Ensemble Classification Methods for Complex Data Stream[J]. Journal of Guangxi Normal University(Natural Science Edition), 2022, 40(4): 1 -21 .
[5] TONG Lingchen, LI Qiang, YUE Pengpeng. Research Progress and Prospects of Karst Soil Organic Carbon Based on CiteSpace[J]. Journal of Guangxi Normal University(Natural Science Edition), 2022, 40(4): 22 -34 .
[6] WANG Dangshu, YI Jiaan, DONG Zhen, YANG Yaqiang, DENG Xuan. Research on Bridgeless Boost PFC Converter with Ripple Suppression Unit Based on Single Cycle Control[J]. Journal of Guangxi Normal University(Natural Science Edition), 2022, 40(4): 47 -57 .
[7] YU Siting, PENG Jingjing, PENG Zhenyun. Rank Constraint Least Square Symmetric Semidefinite Solutions and Its Optimal Approximation of the Matrix Equation[J]. Journal of Guangxi Normal University(Natural Science Edition), 2022, 40(4): 136 -144 .
[8] QIN Chengfu, MO Fenmei. Structure ofC3-and C4-Critical Graphs[J]. Journal of Guangxi Normal University(Natural Science Edition), 2022, 40(4): 145 -153 .
[9] YIN Yudong, KE Shanzhe, HUANG Jiayan, DENG Mengxiang, LIU Guanyan, CHENG Keguang. One-pot Generation of Allylated Products from Alcohols, Carboxylic Acids and Amines with 1,3-Dibromopropane by Sodium Hydride[J]. Journal of Guangxi Normal University(Natural Science Edition), 2022, 40(4): 154 -161 .
[10] DU Libo, LI Jinyu, ZHANG Xiao, LI Yonghong, PAN Weidong. Chemical Constituents and Biological Activity from the Bark of Toona ciliata var. pubescens[J]. Journal of Guangxi Normal University(Natural Science Edition), 2022, 40(4): 162 -172 .
Copyright © Journal of Guangxi Normal University(Natural Science Edition), All Rights Reserved.
Tel: 0773-5857325　E-mail: gxsdzkb@mailbox.gxnu.edu.cn
Powered by Beijing Magtech Co. Ltd