mSdr9c7基因在C57BL/6J小鼠不同组织中表达水平的研究

doi:10.16088/j.issn.1001-6600.2020070901

Abstract

Abstract: The expression level of mSdr9c7 gene in the tissues of C57BL/6J male adult mice was detected for further research on its function. The suitable forward primer sequence as GCCTGACGTCAAGTTCT and the backward primer sequence as TTCTAGAGGCCCCAGCAGAT for real-time fluorescence quantitative PCR were screened to detect the expression level of mSdr9c7 gene. Five 18-week-old C57BL/6J male mice were used as experimental animals. 16 kinds of tissues or cells such as skin,liver,testis,bone marrow,blood vessels,cerebellum,cerebellum,kidney,blood,lung,duodenum,spleen,stomach,fat,muscle and heart were collected,and red blood cells and lymphocyte were isolated from peripheral blood. The real-time fluorescent quantitative PCR method was used to detect the expression of mSdr9c7 gene mRNA in the above-mentioned mouse tissues or cells. The gene expression the corresponding liver tissues of each mouse was used as a reference and statistically analyzed. The results showed that mSdr9c7 mRNA was highly expressed in skin,liver,testis and bone marrow,which was 1.069±0.098 9,1.010±0.050 8,0.987±0.081 0 and 0.822±0.083 8 times as that in liver tissue respectively. It expressed at a moderate level in blood vessels,lymphocyte,brain,blood,kidney,lung,duodenum,stomach,red blood cells and spleen,and they were 0.697±0.053 0,0.628±0.057 0,0.577±0.099 6,0.522±0.062 2,0.538±0.053 5,0.455±0.071 5,0.439±0.086 9,0.375±0.101 9,0.391±0.032 0,0.377±0.052 0 times as that in liver tissue respectively. Its expression was at a low level in fat,muscle,cerebellum and heart,which was 0.189±0.025 6,0.129±0.034 3,0.118±0.063 9 and 0.052±0.014 0 times as that in liver tissue respectively.

Key words: mSdr9c7 gene, C57BL/6J mice, tissue expression

CLC Number:

LI Yinling, ZHOU Jie, ZHOU Jing, CHEN Qiaoyuan, ZENG Zhipeng, LIN Wanhua. Study on the Expression Level of mSdr9c7 Gene in Different Tissues of C57BL/6J Mice[J].Journal of Guangxi Normal University(Natural Science Edition), 2021, 39(1): 148-155.

References

[1] KALLBERG Y,OPPERMANN U,JÖRNVALL H,et al. Short-chain dehydr nase/reductase (SDR) relationships: a large family with eight clusters common to human,animal,and plant genomes[J]. Protein Science: a Publication of the Society,2002,11(3): 636-641. DOI:10.1110/ps.26902.
[2] PERSSON B,KALLBERG Y. Classification and nomenclature of the superfamily of short-chain dehydrogenases/reductases (SDRs)[J]. Chemico-Biological Interactions,2013,202(1/2/3): 111-115. DOI:10.1016/j.cbi.2012.11.009.
[3] KOWALIK D,HALLER F,ADAMSKI J,et al. In search for function of two human orphan SDR enzymes: hydroxysteroid dehydrogenase like 2 (HSDL2) and short-chain dehydrogenase/reductase-orphan (SDRO)[J]. The Journal of Steroid Biochemistry and Molecular Biology,2009,117(4/5): 117-124. DOI:10.1016/j.jsbmb.2009.08.001.
[4] CHEN W G,SONG M S,NAPOLI J L. SDR-O: an orphan short-chain dehydrogenase/reductase localized at mouse chromosome 10/human chromosome 12[J]. Gene,2002,294 (1/2):141-146. DOI:10.1016/s0378-1119(02)00757-6.
[5] SHIGEHARA Y,OKUDA S,NEMER G,et al. Mutations in SDR9C7 gene encoding an enzyme for vitamin a metabolism underlie autosomal recessive congenital ichthyosis[J]. Human Molecular Genetics,2016,25(20): 4484-4493. DOI:10.1093/hmg/ddw277.
[6] TAKEICHI T,NOMURA T,TAKAMA H,et al. Deficient stratum corneum intercellular lipid in a Japanese patient with lamellar ichthyosis with a homozygous deletion mutation in SDR9C7[J]. British Journal of Dermatology,2017,177 (3): e62-e64. DOI:10.1111/bjd.15315.
[7] HOTZ A,FAGERBERG C,VAHLQUIST A,et al. Identification of mutations in SDR9C7 in six families with autosomal recessive congenital ichthyosis[J]. British Journal of Dermatology,2018,178 (3): e207-e209. DOI:10.1111/bjd.15994.
[8] KARIM N,MURTAZA G,NAEEM M. Whole-exome sequencing identified a novel frameshift mutation in SDR9C7 underlying autosomal recessive congenital ichthyosis in a Pakistani family[J]. British Journal of Dermatology,2017,177 (5): e191-e192. DOI:10.1111/bjd.15535.
[9] YOUSSEFIAN L,VAHIDNEZHAD H,SAEIDIAN A H,et al. Autosomal recessive congenital ichthyosis: Genomic landscape and phenotypic spectrum in a cohort of 125 consanguineous families[J]. Human Mutation,2019,40(3):288-298. DOI:10.1002/humu.23695.
[10] SEIDL-PHILIPP M,SCHOSSIG A S,MOOBRUGGER-MARTINZ V,et al. Impaired epidermal barrier in autosomal recessive congenital ichthyosis (ARCI) caused by missense mutations in SDR9C7 in two Austrian sisters[J]. Journal of the German Society of Dermatology: JDDG,2019,17(7):742-745. DOI:10.1111/ddg.13843.
[11] TAKEICHI T,HIRABAYASHI T,MIYASAHA Y,et al. SDR9C7 catalyzes critical dehydrogenation of acylceramides for skin barrier formation[J]. The Journal of Clinical Investigation,2020,130(2): 890-903. DOI:10.1172/JCI130675.
[12] 周洁,曾志鹏,李金月,等. HepG2细胞中SDR9C7蛋白的亚细胞定位研究[J]. 广西师范大学学报(自然科学版),2020,38(1):102-106. DOI:10.16088/j.issn.1001-6600.2020.01.013.
[13] 曾志棚,周洁,卢伟敏,等. Sdr9c7^+/-小鼠的生长及高脂饲喂条件下血液生理生化指标分析[J].广西师范大学学报(自然科学版),2020,38(3):104-109. DOI:10.16088/j.issn.1001-6600.2020.03.013.
[14] KAVANAGH KL,JORNVALL H,PERSSON B,et al. The SDR superfamily: functional and structural diversity within a family of metabolic and regulatory enzymes[J]. Cellular and Molecular Life Sciences,2008,65(24): 3895-3906. DOI:10.1007/s00018-008-8588-y.
[15] STEWART P M, BOULTONA, KUMAR S, et al.Cortisol metabolismin human obesity: impaired cortisone→cortisol conversion in subjects with central adiposity[J]. The Journal of Clinical Endocrinology and Metabolism,1999,84(3): 1022-1027. DOI:10.1210/jcem.84.3.5538.
[16] OPPERMANN U C,SALIM S,TJERNBERG L O,et al. Binding of amyloid beta-peptide to mitochondrial hydroxyacyl-CoA dehydrogenase (ERAB): regulation of an SDR enzyme activity with implications for apoptosis in Alzheimer's disease[J]. FEBS Letters,1999,451(3): 238-242. DOI:10.1016/s0014-5793(99)00586-4.
[17] CHANG N S,SCHULTZ L,HSU L J,et al. 17β-Estradiol upregulates and activates WOX1/WWOXv1 and WOX2/WWOXv2 in vitro: potential role in cancerous progression of breast and prostate to a premetastatic state in vivo[J]. Oncogene,2005,24(4): 714-723. DOI:10.1038/sj.onc.1208124.
[18] TANG S H,GAO L C,BI Q,et al. SDR9C7 Promotes lymph node metastases in patients with esophageal squamous cell carcinoma[J]. PLoS One,2013,8(1): e52184-e52192. DOI:10.1371/journal.pone.0052184.
[19] 汤善宏. 基于表达谱芯片技术的食管癌转移相关基因筛选及功能鉴定[D]. 西安: 第四军医大学,2013.
[20] TAKEICHI T. SDR9C7 plays an essential role in skin barrier function by dehydrogenating acylceramide for covalent attachment to proteins[J]. Journal of Dermatological Science,2020,98(2): 82-87. DOI:10.1016/j.jdermsci.2020.03.005.

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Study on the Expression Level of mSdr9c7 Gene in Different Tissues of C57BL/6J Mice

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