Journal of Guangxi Normal University(Natural Science Edition) ›› 2021, Vol. 39 ›› Issue (1): 156-164.doi: 10.16088/j.issn.1001-6600.2020083102

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Cloning and Bioinformatics Analysis of LAP Gene from Pseudomonas

GUO Chen1, ZHOU Fei 2,3, HAN Biao1, PAN Cui1, WU Jiemin1, YANG Ting2,3, SHANG Changhua2,3*   

  1. 1. Scientific Research Academy of Guangxi Environmental Protection, Nanning Guangxi 530022, China;
    2. College of Life Science, Guangxi Normal University, Guilin Guangxi 541006, China;
    3. Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection (Guangxi Normal University), Ministry of Education, Guilin Guangxi 541006, China
  • Received:2020-08-31 Revised:2020-09-22 Online:2021-01-25 Published:2021-03-27

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Abstract: To explore the molecular structure and functional characteristics of LAP gene encoding Leucine aminopeptidases (LAPs) and its role in the process of growth and development, the Pseudomonas Cr13 LAP gene was cloned and characterized by bioinformatics in this study. Firstly, the core fragment of LAP was cloned. Secondly, the full-length DNA of LAP gene was cloned by two Genome Walking. Lastly, LAP gene was analyzed by bioinformatics software. The result showed that the full-length DNA of LAP gene was 1 491 bp in Pseudomonas Cr13. Bioinformatics analysis indicated that the LAP gene was located in the mitochondrion. The LAP gene had a conserved cytoplasmic aminopeptidase motif NTDAEGRL. The Pseudomonas Cr13 LAP promoter (426 bp) contained the basic elements such as TATA-Box and CAAT-box and some potential cis-acting element. Cloning of full-length DNA laid a good foundation for further research on LAP gene function. Cloning and characterization of the promoter region of Pseudomonas sp. Cr13 LAP gene provided substantial basis for further research on the mechanism of regulation and expression of LAP gene.

Key words: Pseudomonas, leucine aminopeptidase, genome walking, bioinformatics

CLC Number: 

  • Q933
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