整合UPLC-QTRAP-MS/MS、网络药理学与细胞实验探讨调气柔肝散结丸抗肝癌血管生成及转移的分子机制

doi:10.16088/j.issn.1001-6600.2026011302

Abstract

Abstract: This study aimed to investigate the active components and molecular mechanism of Tiaoqi Rougan Sanjie Pill (TRSP) in inhibiting angiogenesis, migration and invasion of hepatocellular carcinoma in vitro. A total of 42 chemical components were identified by UPLC-QTRAP-MS/MS; furthermore, based on network pharmacology, 128 potential targets related to HCC metastasis were screened, and four core targets (HIF-1α, TNF, CASP3, and SRC) were identified by topological analysis, with HIF-1α occupying a central position in the network and being identified as the core target. Molecular docking showed that multiple components in TRSP exhibited good binding activity with HIF-1α, with binding energies all below -20.9 kJ/mol. In vitro experiments confirmed that TRSP dose-dependently inhibited the proliferation, migration and invasion of hepatoma cells (HepG2 and MHCC-97H), and significantly suppressed the angiogenic ability of human umbilical vein endothelial cells (HUVECs). Mechanistic studies revealed that TRSP downregulated the protein expression of HIF-1α and its downstream factor VEGFA in hepatoma cells. In conclusion, TRSP may exert anti-angiogenic and anti-metastatic effects against HCC by inhibiting the HIF-1α/VEGF signaling pathway, providing experimental evidence for its clinical application.

Key words: Tiaoqi Rougan Sanjie Pills, liver cancer, angiogenesis, migration, invasion, HIF-1α/VEGF signaling pathway

CLC Number: R285

Wu Lichuan, Sun Yilin, Xie Yuxin, Qin Jianfeng, Xu Liba, LongFuli, Wei Jinrui. Integrating UPLC-QTRAP-MS/MS, network pharmacology, and cellular assays to investigate the molecular mechanism of Tiaoqi Rougan Sanjie Pill in inhibiting angiogenesis and metastasis of hepatocellular carcinoma[J].Journal of Guangxi Normal University(Natural Science Edition), 2026, 44(6): 195-210.

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Integrating UPLC-QTRAP-MS/MS, network pharmacology, and cellular assays to investigate the molecular mechanism of Tiaoqi Rougan Sanjie Pill in inhibiting angiogenesis and metastasis of hepatocellular carcinoma

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