Journal of Guangxi Normal University(Natural Science Edition) ›› 2024, Vol. 42 ›› Issue (5): 163-173.doi: 10.16088/j.issn.1001-6600.2023111601

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Shechuang-Kushen Decoction Inhibit Effect and Mechanism of Compound 48/80 on Itch in ICR Mice

WU Shijian1,2,3, HUANG Guangxian4, SONG Qianqian4, HUANG Chunhua4, LIU Jiamin4, WU Guanyi1,2,3*   

  1. 1. School of Basic Medicine, Guangxi University of Chinese Medicine, Nanning Guangxi 530299, China;
    2. Guangxi Key Laboratory of Translational Medicine for Treating High-Incidence Infectious Diseases with Integrative Medicine (Guangxi University of Chinese Medicine), Nanning Guangxi 530299, China;
    3. Key Laboratory of Characteristic Experimental Animal Models of Guangxi Universities (Guangxi University of Chinese Medicine), Nanning Guangxi 530299, China;
    4. School of Pharmacy, Guangxi University of Chinese Medicine, Nanning Guangxi 530200, China
  • Received:2023-11-16 Revised:2023-12-04 Online:2024-09-25 Published:2024-10-11

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Abstract: Common Cnidium and Sophora flavescens are commonly used antipruritic herbs,but their combined effect on itch has not been fully elucidated. In this study,ICR mice were gavaged with Shechuang-Kushen Decoction,and the effect of the drug on the pruritus behavior of the mice was observed by a behavioral method. The changes in vascular permeability were observed by Evans Blue staining. The effect of the decoction on the release of cytokines from the mast cell line RBL-2H3 was evaluated by ELISA and Western Blotting,respectively. The results showed that the decoction inhibited pruritus behavior and increased vascular permeability,but did not affect the exploration ability or muscle tone of the mice. The decoction solution reduced the release of β-hexosaminidase,histamine,IL-31,IL-4,IL-13,and CXCL10,and inhibited the phosphorylation of the ERK protein in RBL-2H3 cells. This study demonstrated that Shechuang-Kushen Decoction inhibited compound 48/80-induced acute pruritus by inhibiting RBL-2H3 cell degranulation and the release of pruritus-related cytokines.

Key words: Common Cnidium, Sophora flavescens, itch, relieve itching

CLC Number:  R285.5
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