广西师范大学学报（自然科学版） ›› 2026, Vol. 44 ›› Issue (6): 257-267.doi: 10.16088/j.issn.1001-6600.2026012903

• 分子生物学与生物技术 • 上一篇    

高产四甲基吡嗪菌株Bacillus sp. TTMP20的分离及其基因组分析

李雨佳1,2, 欧见梅1,2, 玉石松1,2, 尚常花1,2*   

  1. 1.广东省可再生能源重点实验室, 广东 广州 510640;
    2.广西师范大学 生命科学学院, 广西 桂林 541006
  • 收稿日期:2026-01-29 修回日期:2026-03-27 出版日期:2026-11-05 发布日期:2026-11-10
  • 通讯作者: 尚常花(1980—), 男, 山西泽州人, 广西师范大学副教授, 博士。E-mail: shangchanghua@gxnu.edu.cn
  • 基金资助:
    广东省可再生能源重点实验室开放基金(E539kf1301);广西重点研发计划(2023AB01132)

Isolation and genomic analysis of a high tetramethylpyrazine yield Bacillus sp. TTMP20

Li Yujia1,2, Ou Jianmei1,2, Yu Shisong1,2, Shang Changhua1,2*   

  1. 1. Guangdong Provincial Key Laboratory of Renewable Energy, Guangzhou Guangdong 510640, China;
    2. College of Life Sciences, Guangxi Normal University, Guilin Guangxi 541006, China
  • Received:2026-01-29 Revised:2026-03-27 Online:2026-11-05 Published:2026-11-10

摘要： 四甲基吡嗪是药用活性物质及白酒中的风味物质。本研究从丹泉白酒的大曲和酒糟中,筛选出一株高产四甲基吡嗪(3 896.36 mg/L)菌株——芽孢杆菌TTMP20 (Bacillus sp. TTMP20)。为更好地了解TTMP20生产四甲基吡嗪的机制,并进行TTMP20基因工程改造,本研究对TTMP20进行基因组测序,阐述TTMP20基因组特征,详细分析四甲基吡嗪合成的相关基因与途径,对基因进行KEGG、COG、GO、NR和CAZy注释。这些途径涵盖果糖代谢途径、淀粉和蔗糖代谢途径、糖酵解途径、磷酸戊糖途径,以及部分丁酸代谢途径与氮代谢途径。结果显示,TTMP20具备完整的乙偶姻和四甲基吡嗪代谢途径,能利用多种复杂碳源。基因组信息进一步深入揭示了基因与四甲基吡嗪生成之间的关系。

关键词: 四甲基吡嗪, 芽孢杆菌TTMP20, 基因组测序, 代谢途径, 活性物质, 风味物质

Abstract: Tetramethylpyrazine is a pharmaceutically active substance and a flavor substance in Baijiu. A strain (Bacillus sp. TTMP20) with a high tetramethylpyrazine yield (3 896.36 mg/L) was screened from Daqu and fermented grains of Danquan Baijiu in this study. To better understand the mechanism of tetramethylpyrazine production by TTMP20 and perform its genetic modification, genome sequencing of TTMP20 was conducted. This study described the genomic characteristics of TTMP20. It analyzed genes and pathways associated with tetramethylpyrazine synthesis in detail. Gene function was analyzed by KEGG, COG, GO, NR and CAZy annotation. These pathways included fructose metabolism pathway, pathways of starch and sucrose metabolism, glycolysis pathway, pentose phosphate pathway, partial butanoate metabolism pathway and nitrogen metabolism pathway. Results showed that Bacillus sp. TTMP20 had complete metabolic pathways for acetoin and tetramethylpyrazine, and TTMP20 could utilize multiple complex carbon sources. Genome information thoroughly revealed the connection between genes and tetramethylpyrazine production.

Key words: tetramethylpyrazine, Bacillus sp. TTMP20, genome sequencing, metabolic pathways, active substances, flavor compounds

中图分类号:  Q933

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