广西师范大学学报（自然科学版） ›› 2026, Vol. 44 ›› Issue (3): 180-191.doi: 10.16088/j.issn.1001-6600.2025040706

• 分子生物学与生物技术 • 上一篇    下一篇

全球霍乱弧菌耐药基因的分布、移动性及扩散格局分析

邹忠爱1,2, 丘国铮2, 何思月2, 黄诗晴2, 钱弈秋2, 刘宗保2,3*   

  1. 1.厦门华厦学院 环境与公共健康学院, 福建 厦门 361024;
    2.珍稀濒危动植物生态与环境保护教育部重点实验室(广西师范大学), 广西 桂林 541006;
    3.广西漓江流域景观资源保育与可持续利用重点实验室(广西师范大学), 广西 桂林 541006
  • 收稿日期:2025-04-07 修回日期:2025-05-16 出版日期:2026-05-05 发布日期:2026-05-13
  • 通讯作者: 刘宗保(1985—), 男, 山东临沂人, 广西师范大学教授, 博士。E-mail: zongbaoliu@gxnu.edu.cn
  • 基金资助:
    厦门市自然科学基金(3502Z20227231);广西科技基地和人才专项(2022AC21335)

Global Distribution, Mobility, and Dissemination Patterns of Antimicrobial Resistance Genes in Vibrio cholerae

ZOU Zhongai1,2, QIU Guozheng2, HE Siyue2, HUANG Shiqing2, QIAN Yiqiu2, LIU Zongbao2,3*   

  1. 1. College of Environment and Public Health, Xiamen Huaxia University, Xiamen Fujian 361024, China;
    2. Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection (Guangxi Normal University), Guilin Guangxi 541006, China;
    3. Guangxi Key Laboratory of Landscape Resources Conservation and Sustainable Utilization in Lijiang River Basin (Guangxi Normal University), Guilin Guangxi 541006, China
  • Received:2025-04-07 Revised:2025-05-16 Online:2026-05-05 Published:2026-05-13

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摘要： 细菌耐药性已成为全球公共卫生的重大挑战,霍乱弧菌Vibrio cholerae作为一种主要的致病菌,其耐药性问题日益严重。本文基于全球霍乱弧菌基因组序列数据,利用生物信息学方法对其耐药基因(ARGs)的分布特征、丰度、多样性以及可移动性进行系统分析。通过对81 132条霍乱弧菌基因组序列的筛选与比对,共鉴定出16 570条ARG序列,涵盖83个ARG亚型,涉及19种抗生素类别。其中,多重耐药、四环素类、大环内酯类和磺胺类等ARGs丰度较高,占总体ARG丰度的69.23%。ARGs的多样性与丰度呈现显著地理异质性,中国、印度、海地等中低收入国家的水平显著高于高收入国家。高达91.57%的ARGs亚型与可移动遗传元件(MGEs)相关,凸显了MGEs在ARGs积累与传播中的关键作用。进一步通过聚类分析识别出120个可移动ARGs操作分类单元,其中26.67%在至少3个国家中检出,氨基糖苷类、氯霉素类、磺胺类等ARGs在全球范围内呈现出广泛的地理分布。代表性基因dfrA1、APH(6)-Id和cat3已证实发生洲际扩散,主要集中于亚非地区。本文研究为深入理解霍乱弧菌耐药性特征及其传播机制提供了科学依据,有助于制定有效的监测与防控策略,以应对霍乱弧菌及其他病原体的耐药性挑战。

关键词: 霍乱弧菌, 耐药基因, 基因组, 可移动遗传元件, 全球扩散

Abstract: Antimicrobial resistance (AMR) has emerged as a critical global public health challenge. Vibrio cholerae, a major pathogenic bacterium, is exhibiting increasing resistance to antibiotics, complicating cholera control efforts worldwide. In this study, a comprehensive bioinformatic analysis of the distribution, abundance, diversity, and mobility of antimicrobial resistance genes (ARGs) in V. cholerae based on global genomic datasets is performed. Screening and comparative analysis of 81 132 V. cholerae genome sequences led to the identification of 16 570 ARG sequences, encompassing 83 ARG subtypes associated with 19 antibiotic classes. Notably, genes conferring resistance to multiple drugs, tetracyclines, macrolides, and sulfonamides were the most prevalent, accounting for 69.23% of total ARG abundance. The diversity and abundance of ARGs exhibited significant geographic heterogeneity, with markedly higher levels observed in middle-and low-income countries such as China, India, and Haiti, compared with those in high-income countries. Strikingly, 91.57% of ARG subtypes were associated with mobile genetic elements (MGEs), highlighting their central role in the acquisition and dissemination of resistance. Cluster analysis further identified 120 mobile ARG operational taxonomic units, 26.67% of which were detected in at least three countries. ARGs conferring resistance to aminoglycosides, chloramphenicol, and sulfonamides displayed widespread geographic distribution. Representative genes such as dfrA1, APH(6)-Id, and cat3 have undergone intercontinental dissemination, predominantly across Asia and Africa. This study provides critical insights into the AMR landscape and transmission mechanisms of V. cholerae, offering a scientific basis for the development of effective surveillance and control strategies to mitigate the threat of resistance in V. cholerae and other pathogens.

Key words: Vibrio cholerae, antimicrobial resistance genes, genome, mobile genetic elements, global dissemination

中图分类号:  Q938.2; R378

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