波纹鳜线粒体基因克隆及系统发育信息分析

doi:10.16088/j.issn.1001-6600.2019.04.015

广西师范大学学报（自然科学版） ›› 2019, Vol. 37 ›› Issue (4): 119-126.doi: 10.16088/j.issn.1001-6600.2019.04.015

波纹鳜线粒体基因克隆及系统发育信息分析

杨丹丹¹, 谢永广¹, 宾石玉^2,3, 罗华辉¹, 安苗¹, 陈敦学^1*

1.贵州大学动物科学学院,贵州贵阳550025;
2.广西师范大学生命科学学院,广西桂林541006;
3.珍稀濒危动植物生态与环境保护教育部重点实验室(广西师范大学),广西桂林541006

收稿日期:2018-10-28 出版日期:2019-10-25 发布日期:2019-11-28
通讯作者: 陈敦学(1986—),男,湖北荆州人,贵州大学副教授,博士。E-mail:chendunxue@126.com
基金资助:
国家自然科学基金(31560718,31660741);贵州大学博士基金(贵大人基合字〔2015〕01号)

Mitochondrial Genome Characteristics and Phylogenetic Information of Siniperca undulata

YANG Dandan¹, XIE Yongguang¹, BIN Shiyu^2,3, LUO Huahui¹, AN Miao¹, CHEN Dunxue^1*

1. College of Animal Science, Guizhou University, Guiyang Guizhou 550025, China;
2. College of Life Sciences, Guangxi Normal University,Guilin Guangxi 541006, China;
3. Key Laboratory of Ecology of Rare and Endangered Speicies and Environmental Protection(Guangxi Normal University), Ministsry of Education, Guilin Guangxi 541006, China

Received:2018-10-28 Online:2019-10-25 Published:2019-11-28

摘要/Abstract

摘要： 为了探讨鳜类线粒体基因组结构特征及系统发育信息,以期为鳜类遗传学研究和分子标记提供参考依据,本文将波纹鳜Siniperca undulata线粒体随机切割成长度为1~3 kb的15个片段,采用PCR产物直接测序法获得波纹鳜线粒体全基因组,并提交至NCBI数据库,获得登录号KJ644783。结果分析发现:波纹鳜线粒体包含13个编码蛋白基因、22个tRNA、2个rRNA以及一个超变区(D-loop),基因排列顺序与其他硬骨鱼类似,不存在基因重排现象。整个基因组存在明显的AC偏好性,其中G碱基含量非常低,仅仅为16.23%。系统发育信息分析结果显示,CO1、ND5、CYTB、ND4基因具有较好的进化适应性,能够较好地反映鳜类的系统发育关系。进一步的进化分析显示,鳜类分成了鳜属和少鳞鳜属。

关键词: 波纹鳜, 线粒体基因组, 碱基偏好性, 适应性, 系统进化

Abstract: In order to understand the characteristics and phylogenetic information of the Sinipercids, which may be benefit to the evolutionary genetics and selection of molecular markers, fifteen pairs of primers were used to amplify the contiguous, overlapping segments complete mitochondrial genome of Siniperca undulata. The genomes was deposited in the GenBank with accession numbers KJ644783. The organization of S. undulata mitochondrial genomes was similar to that of other teleost species containing 37 genes (13 protein-coding genes, 2 ribosomal RNA, and 22 transfer RNAs) and a major non-coding control region. Great preference was observed in A and C, the G contents was 16.23% which is relatively low. The results showed that the CO1, ND5, CYTB and ND4 play a good role in analysis of the molecular adaptive evolution. In addition, the phylogeny evolution demonstrated that the Sinipercids which were divided into two genes: Siniperca and Coreoperca.

Key words: Siniperca undulata, mitochondrial, codon preference, informative efficiencies, phylogeny

中图分类号:

S961

杨丹丹, 谢永广, 宾石玉, 罗华辉, 安苗, 陈敦学. 波纹鳜线粒体基因克隆及系统发育信息分析[J]. 广西师范大学学报（自然科学版）, 2019, 37(4): 119-126.

YANG Dandan, XIE Yongguang, BIN Shiyu, LUO Huahui, AN Miao, CHEN Dunxue. Mitochondrial Genome Characteristics and Phylogenetic Information of Siniperca undulata[J]. Journal of Guangxi Normal University(Natural Science Edition), 2019, 37(4): 119-126.

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波纹鳜线粒体基因克隆及系统发育信息分析

Mitochondrial Genome Characteristics and Phylogenetic Information of Siniperca undulata

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