东北地区3种啮齿动物肠道病毒组成的比较研究

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doi:10.16088/j.issn.1001-6600.2023121001

摘要： 棕背Myodes rufocanus、大林姬鼠Apodemus peninsulae和黑线姬鼠Apodemus agrarius是东北地区主要的3种害鼠。动物肠道微生物伴随宿主进化与其胃肠道构成了复杂的微生态系统,并在肠道内发挥着重要作用。为研究3种啮齿动物肠道病毒组成及亲缘关系和生境2种因素对其的影响,本研究对在同一生境下的17只大林姬鼠、17只棕背以及另一生境中的9只黑线姬鼠肠道内容物样本进行宏基因组测序,对3种啮齿动物的肠道病毒组成及差异进行对比与分析。结果显示:Artverviricota、Uroviricota是3种啮齿动物共有的优势病毒门,Betaretrovirus、Gammaretrovirus是3种啮齿动物共有的优势病毒属。此外,3种啮齿动物肠道病毒群落之间存在显著差异,有9个门18个属的病毒在不同样本中得到富集。在肠道相对丰度前10的病毒中,大林姬鼠与黑线姬鼠之间有5个门5个属具有显著差异,棕背与大林姬鼠之间有3个门和6个属具有显著差异。在门水平上棕背与大林姬鼠肠道病毒组成相似度更高,而属水平上则是黑线姬鼠与大林姬鼠肠道病毒组成更高。在门水平上生境对肠道病毒组成的影响较大,在属水平上则是亲缘关系对肠道病毒组成的影响较大。

关键词: 啮齿动物, 肠道病毒, 宏基因组, 东北地区

Abstract: Myodes rufocanus, Apodemus peninsulae and Apodemus agrarius are the three main rodent pests in the Northeast region of China. The gut microbiota of animals, along with their gastrointestinal tract, form a complex microbial ecosystem and play an important role in the host’s intestine. To investigate the composition and phylogenetic relationships of gut viruses in these three rodent species and the impact of their habitats, this study conducted metagenomic sequencing on 17 samples of Korean field mice, 17 samples of brown-backed voles from the same habitat, and 9 samples of striped field mice from a different habitat. After screening for viral information, the gut viral composition and differences among the three rodent species were compared and analyzed. The results showed that Artverviricota and Uroviricota were dominant viral phyla shared by the three rodent species, while Betaretrovirus and Gammaretrovirus were dominant viral genera shared by the three rodent species. Furthermore, significant differences were observed among the gut viral communities of the three rodent species, with nine phyla and 18 genera of viruses enriched in different samples. Among the top ten viruses in terms of relative abundance in the gut, Korean field mice and striped field mice exhibited significant differences in five phyla and five genera, while brown-backed voles and Korean field mice exhibited significant differences in three phylum and six genera. At the phylum level, the gut viral composition of brown-backed voles was more similar to that of Korean field mice, whereas at the genus level, the gut viral composition of striped field mice was more similar to that of Korean field mice. Based on these findings, it can be inferred that habitat had a greater influence on gut viral composition at the phylum level, while factors such as diet, phylogenetic relationship had a greater impact.

Key words: rodent, enterovirus, metagenome, northeast of China

中图分类号: Q939.4

王声泽, 张承志, 徐梦昊, 王妍, 王阳, 李婷, 原宝东. 东北地区3种啮齿动物肠道病毒组成的比较研究[J]. 广西师范大学学报（自然科学版）, 2024, 42(6): 226-235.

WANG Shengze, ZHANG Chengzhi, XU Menghao, WANG Yan, WANG Yang, LI Ting, YUAN Baodong. Comparative Study of Gastrointestinal Viruses in Three Rodent Species in the Northeast of China[J]. Journal of Guangxi Normal University(Natural Science Edition), 2024, 42(6): 226-235.

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