广西师范大学学报（自然科学版） ›› 2020, Vol. 38 ›› Issue (3): 104-109.doi: 10.16088/j.issn.1001-6600.2020.03.013

• • 上一篇    下一篇

Sdr9c7+/-小鼠的生长及高脂饲喂条件下血液生理生化指标分析

曾志棚1,2, 周洁1,2, 卢伟敏3, 陈俏媛1,2, 贺思诺1,2, 林万华1,2*   

  1. 1.广西高校干细胞与医药生物技术重点实验室(广西师范大学),广西桂林541006;
    2.广西师范大学生命科学学院,广西桂林541006;
    3.广西师范大学 校医院,广西桂林541006
  • 收稿日期:2019-03-26 出版日期:2020-05-25 发布日期:2020-06-11
  • 通讯作者: * 林万华(1975—),男,江西南康人,广西师范大学副教授,博士。E-mail: lwh@gxnu.edu.cn
  • 基金资助:
    国家自然科学基金(31560248);广西自然科学基金(2016GXNSFAA380176);广西师范大学博士科研启动基金;广西研究生教育创新计划(XYCSZ2018055)

Growth and Effects of High-fat Diet Feed on Blood Physiological and Biochemical Indicators of Sdr9c7+/- Mice

ZENG Zhipeng1,2, ZHOU Jie1,2, LU Weimin3, CHEN Qiaoyuan1,2, HE Sinuo1,2, LIN Wanhua1,2*   

  1. 1. Guangxi Universities Key Laboratory of Stem Cell and Biopharmaceutical Technology (Guangxi Normal University), Guilin Guangxi 541006, China;
    2. College of Life Sciences, Guangxi Normal University, Guilin Guangxi 541006, China;
    3. Affiliated Hospital, Guangxi Normal University, Guilin Guangxi 541006, China
  • Received:2019-03-26 Online:2020-05-25 Published:2020-06-11

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摘要： 以野生型(Sdr9c7+/+ )和杂合子型(Sdr9c7+/-)雄性小鼠为实验动物分析普通饲料和高脂饲料饲喂条件下 Sdr9c7基因对小鼠生长发育的影响,并检测高脂饲喂条件下小鼠血液生理生化指标及肝脏脂肪变性情况。结果表明:1)与Sdr9c7+/+小鼠相比,Sdr9c7+/-小鼠发育正常,二者生长曲线无显著差异;2)高脂饲料喂养至15周龄时,两组间小鼠血液的葡萄糖(GLU)、血清总胆固醇(TCHO)、甘油三酯(TG)、低密度胆固醇(LDL)、谷丙转氨酶(ALT)、谷草转氨酶(AST)、尿素(BUN)、肌酐(CREA)含量等无差异;至28周龄时,杂合子组小鼠血清的AST和ALT含量显著升高(P<0.05),推测Sdr9c7与肝炎症相关。但肝脏系数和肝脏石蜡组织切片中细胞空泡面积无明显差异,说明Sdr9c7基因对肝脏脂肪变性没有影响。

关键词: Sdr9c7基因, 生长发育, 高脂喂食, 血液生理生化指标, 脂肪肝

Abstract: Wild type (Sdr9c7+/+) and heterozygote type (Sdr9c7+/-) male mice were used to analyze the effects of Sdr9c7 gene on the growth and development under regular chow diets and high-fat diets, and the physiological and biochemical indicators of blood and liver steatosis of mice under high-fat diets were also measured. Results: (1) Compared with Sdr9c7+/+ mice, Sdr9c7+/- mice developed normally, and there was no significant difference in their body weight curves. (2) When the mice fed with high-fat diet to 15 weeks of age, there was no difference in the level of blood glucose (GLU), serum total cholesterol (TCHO), triglyceride (TG), low-density cholesterol (LDL), alanine aminotransferase (ALT), alanine aminotransferase (AST), urea (BUN) and creatinine (CREA) of the mice between the two groups. At the age of 28 weeks under high-fat diets, the AST and ALT levels in the heterozygote group were significantly increased (P<0.05), suggesting that Sdr9c7 might be associated with hepatitis, but there was no significant difference in liver coefficient and cell vacuole area of paraffin section, indicating that Sdr9c7 gene had no effect on hepatic steatosis.

Key words: Sdr9c7 gene, growth and development, high-fat feed, blood physiological and biochemical indicators, fatty liver

中图分类号: 

  • Q592.1
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