广西师范大学学报（自然科学版） ›› 2015, Vol. 33 ›› Issue (4): 144-151.doi: 10.16088/j.issn.1001-6600.2015.04.023

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耐力运动抗非酒精性脂肪肝的形成作用及其机制

拓万亮1, 彭峰林2   

  1. 1.塔里木大学体育工作部,新疆阿拉尔843300;
    2.广西师范大学体育学院,广西桂林541006
  • 收稿日期:2015-06-02 出版日期:2015-12-25 发布日期:2018-09-21
  • 通讯作者: 拓万亮(1980—),男,新疆阿拉尔人,塔里木大学讲师,硕士。E-mail: tuowanliang@126.com
  • 基金资助:
    国家自然科学基金资助项目(31060146)

Effects on Lipid Metabolism,Antioxidant Capacity and Non-Alcoholic Fatty Liver Disease Formation in Rats with High Fat Diet

TUO Wan-liang1, PENG Feng-lin2   

  1. 1. Department of Sports Work, Tarim University, Alaer Xinjiang 843300,China;
    2. College of Physical Education,Guangxi Normal University,Guilin Guangxi 541006, China
  • Received:2015-06-02 Online:2015-12-25 Published:2018-09-21

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摘要： 为比较并探讨不同强度耐力运动抗高脂饮食大鼠非酒精性脂肪肝(NAFLD) 形成作用及其机制,将成年雄性SD 大鼠50只随机分为:普通饮食对照组(C 组)、高脂饮食对照组 (H 组)、高脂饮食大强度运动组 (Hh组)、高脂饮食较大强度运动组( Hv 组)和高脂饮食中强度运动组(Hm组)。除C组外,其余各组采用高脂饮食喂养7周,同时采用相应强度的耐力运动进行干预,观察各组大鼠脂肪肝病变情况,比较血清ALT、AST及TC、TG、FFC水平变化,比较肝组织SOD2 mRNA表达水平,比较血及肝组织T-SOD、MDA水平变化,结果发现:高脂饮食大鼠血清TC、TG、FFC浓度升高,肝组织SOD2 mRNA表达水平显著升高,血及肝组织T-SOD活性下降、MDA含量升高。肉眼下及HE染色的肝组织表现出NAFLD样形态学病变,肝功指标即血清ALT、AST浓度显著升高。但比起H组,Hh 组、Hv组、Hm组的肝组织SOD2 mRNA表达水平进一步升高,上述异常变化得到不同程度的连锁样缓解,其中Hm组最明显。综上可见:不同强度的耐力运动均可改善高脂饮食大鼠血脂代谢异常,提高肝组织SOD2 mRNA表达,提高血、肝组织T-SOD活性,减轻肝脂质过氧化反应程度,最终发挥抗NAFLD形成作用,保护肝功能,但中等强度耐力运动调血脂及抗NAFLD形成的效果最佳。

关键词: 运动强度, 耐力运动, 非酒精性脂肪肝, 脂质过氧化

Abstract: To explore and compare the effects of high intensity, vigorous intensity and moderate intensity endurance exercise on Blood Lipid, Free Radical metabolism, and its relationship with Non-Alcoholic Fatty Liver Disease (NAFLD),50 grown male SD rats were randomly divided into normal diet control group (Group C), high-fat diet control group (Group H), high-fat diet and high intensity endurance exercise group (Group Hh), high-fat diet and vigorous intensity endurance exercise group (Group Hv), high-fat diet and moderate intensity endurance exercise group (Group Hm). Except group C, the other groups were fed with high-fat diet. At the same time, the groups which were fed with high-fat diet were intervened by high, vigorous or moderately intense endurance exercise. Indexs have been observed including the Fatty Liver Disease lesions existed in rats, and detected serum Alanine Aminotransferase (ALT), Aspartate Aminotransferase (AST),Total Cholesterol (TC), Triglyceride (TG), Free Fatty Acid(FFA)indexs, and detected liver SOD2 mRNA and serum, liver T-Superoxide Dismutase (T-SOD), Malondialdehyde (MDA). The results show that serum ALT, AST and TC, TG, FFA levels increased significantly in rats fed with high-fat diet, and serum and liver T-SOD level decreased significantly, serum and liver MDA level increased significantly, and liver SOD2 mRNA increased significantly. In the meanwhile, Liver Index increased significantly, and the Liver Tissue under the naked eye, the Hematoxylin-Eosin (HE) put up Fatty Liver Disease morphology lesion. Compared with Group H, Group Hh, Group Hv and Group Hm mRNA increased significantly ,and the above abnormal changes for different degrees got the chain-like relief in Group Hh, Group Hv and Group Hm, and the most obvious changes occured in Group Hm. It draw a conclusion that different intensity endurance exercise can improve the abnorm of lipids metabolism, improve expression of SOD2 mRNA ,increase the activity of T-SOD, ruduce lipid peroxidation, and finatly, prevent NAFLD formation and protect liver function. Above all, moderate intensity get the best effect.

Key words: exercise intense, endurance exercise, non-alcoholic fatty liver disease, lipid metabolism

中图分类号: 

  • G804.2
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